Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs

Rimhanen-Finne, Ruska; Enemark, Heidi L.; Kolehmainen, Juha; Toropainen, P.; Hänninen, Marja‐Liisa

doi:10.1016/j.vetpar.2007.01.008

Cited by 66 publications

(52 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…was also (26.9%). The prevalence of intestinal protozoan observed in the present study was higher than the overall prevalence of intestinal protozoan reported elsewhere; in Australia (9.4%) (Palmer et al, 2008), Greece (4.3%) (Papazahariadou et al, 2007), Finland (5%) (Rimhanen-Finne et al, 2007), United States (4%) (Little et al, 2009). The difference in prevalence may probably have resulted from difference in geographical locations and high standard of living.…”

Section: Resultscontrasting

confidence: 69%

“…Worldwide, there are many studies on prevalence of intestinal protozoan parasites in dogs (Oliveira-Sequeira et al, 2002;Ramirez-Barrios et al, 2004;Dubna et al, 2007;Mundim et al, 2007;Papazahariadou et al, 2007;Rimhanen-Finne et al, 2007;Palmer et al, 2008;Little et al, 2009) with the estimate prevalences of dog intestinal parasites varying from 5 to 70% (Blagburn et al, 1996;Bugg et al, 1999). Prevalence is variable and depended on a number of factors including age, living conditions, diagnostic methodology employed and region studied (Mundim et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of intestinal protozoan parasites in stray and domicile dogs in Ilorin, North Central, Nigeria

Adedoja¹,

Oshodi²,

Akanbi³

et al. 2015

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

Uncontrolled population of stray and domicile dogs with intestinal protozoan in close proximity to increasing densities of human population in urban environments is a common fact in developing countries, in conjunction with the lack of veterinary attention and zoonotic awareness, increases the risks of disease transmission. We examined using standard method 108 stool specimens of stray and domicile dogs collected randomly from Ilorin, state capital of Kwara State, Nigeria for intestinal protozoan. The prevalence of intestinal parasites from both stray and domicile dogs was 64(59.3%). Three parasites were encountered; Anchylostoma sp, Cryptosporidium sp and Isospora sp. Female dogs were more infected (48.9%) than male dogs (11.1%) (P<0.001) while the stray dogs had more intestinal parasitic infection (68.3%) than domicile dogs (46.7). More of Cryptosporidium sp was observed among strayed dogs (36.5%). Double infection was highest in stray dogs (12.7%) than in domicile dogs (4.4%) but not statistically significant (p=0.051). Consistent sanitary education must be included in public health government actions as a first step for the control of intestinal parasites in dogs and good sanitation and hygiene should be encouraged minimize environmental contamination and contact with the infectious oocysts that may be shed by dogs.

show abstract

Section: Resultscontrasting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Prevalence of intestinal protozoan parasites in stray and domicile dogs in Ilorin, North Central, Nigeria

Adedoja¹,

Oshodi²,

Akanbi³

et al. 2015

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

show abstract

“…DFA detected cysts of G. duodenalis in wild boars, foxes, roe deer and wolves, whereas PCR was positive for samples from wild boars, red deer, moose, roe deer, wolves and European badger. The number of cysts was generally quite low (less than ten cysts per slide); this may reflect an asymptomatic infection or carriage of G. duodenalis, which occurs frequently among animals (Rimhanen-Finne et al 2007). …”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular typing of Giardia duodenalis in wildlife from eastern Poland

Stojecki

Sroka

Cacciò

et al. 2015

Folia Parasitologica

View full text Add to dashboard Cite

Faecal samples from 162 wild animals were collected from 32 distinct sites of Łęczyńsko-Włodawskie Lakeland (eastern Poland). The presence of Giardia duodenalis (Stiles, 1902) was assessed by a Direct Fluorescence Assay (DFA) and by Polymerase Chain Reaction (PCR) and sequencing of a fragment of the beta-giardin gene. DFA showed the presence of cysts of G. duodenalis in 12 of 162 faecal samples (7%), namely in four wild boars (15%), four foxes (19%), two roe deer (4%), and two wolves (29%). PCR identified 34 of the 162 (21%) samples as positive, including 11 wild boars (41%), five red deer (18%), 11 roe deer (23%), four moose (17%), two wolves (29%) and a single sample from the European badger. Thus, PCR detected a significantly higher number of infection than DFA (P = 0.0005). However, 14 of 34 PCR products could not be sequenced because of their insufficient amount; the low number of cysts, poor conservation of the faeces or presence of PCR inhibitors may have contributed to weak DNA amplification. Sequence analysis of the remaining 20 products showed the presence of assemblage B in wild boars, red deer and roe deer, whereas samples from wolves were identified as assemblage D. This is the first detection of assemblage B in wild boars and deer. As assemblage B has zoonotic potential, wild animals from eastern Poland may act as reservoirs of cysts of G. duodenalis infectious for humans.

show abstract

“…Immunochromatography methods (IC, dipsticks) provide a faster and more convenient diagnostic alternative in feces with no preservative. Several studies have reported similar sensitivity of Giardia detection in feces through IF and ELISA, and noticeably greater than the sensitivity shown by IC, particularly in feces with low Giardia cyst count [9,[116][117][118]. Molecular methods have shown exquisite sensitivity and specificity.…”

Section: Diagnosis Of Giardia Infectionmentioning

confidence: 99%

“…Molecular methods have shown exquisite sensitivity and specificity. Several authors have shown that polymerase chain reaction (PCR) increases 10 4 times detection sensitivity as compared to IF [117]. The absence of differential morphological features of the parasite makes its identification at species level difficult; therefore, only molecular techniques allow the identification of zoonotic genotypes of Giardia [2,9].…”

Section: Diagnosis Of Giardia Infectionmentioning

confidence: 99%

Giardia duodenalis: NEW INSIGHTS ON AN ANCIENT PARASITE

NB¹,

Ja²

2013

Int J Parasitol Res

View full text Add to dashboard Cite

Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs

Cited by 66 publications

References 19 publications

Prevalence of intestinal protozoan parasites in stray and domicile dogs in Ilorin, North Central, Nigeria

Prevalence of intestinal protozoan parasites in stray and domicile dogs in Ilorin, North Central, Nigeria

Prevalence and molecular typing of Giardia duodenalis in wildlife from eastern Poland

Giardia duodenalis: NEW INSIGHTS ON AN ANCIENT PARASITE

Contact Info

Product

Resources

About