Evaluation of human cytomegalovirus antigen expression in invasive breast carcinoma in a population of Iranian patients

Mohammadizadeh, Fereshteh; Mahmudi, Fatemeh

doi:10.1186/s13027-017-0148-3

Cited by 13 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, in our study the HCMV IE antigen expression was not detected in any of the cases and control group indicating no association between HCMV infection and ductal breast carcinoma. Similar negative results were reported by Mohammadizadeh et al [21] from Iran who investigated HCMV IE protein expression in 70 breast cancer and 26 normal breast tissues samples using the IHC method and real-time PCR. Another study by Utrera Barillas et al [ 22] in Mexico evaluated 27 breast cancer specimens and 20 fibroadenoma samples by quantitative PCR and reported no significant association between HCMV and breast cancer development.…”

Section: Discussionsupporting

confidence: 82%

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

Aidrous

Hussein²,

Dahawi

et al. 2019

NACS

View full text Add to dashboard Cite

Background: Breast cancer is a leading cause of death among women worldwide. The association between Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV) and breast cancer risk still remains controversial making it difficult to determine whether either, both or neither virus is causally associated with breast cancer. The aim of this study was to detect EBV and the expression of the Immediate Early Antigen (IE) of HCMV in breast cancer in Sudanese women.Method: 60 Formalin-fixed and paraffin-embedded tissue blocks from 42 ductal breast carcinoma and18 benign breast tumors (control group) were obtained from the pathology laboratories of several hospitals in Khartoum State, Sudan, and used in this study. We used PCR to detect EBV and the IE-antigen of HCMV in ductal breast carcinoma and benign breast tumor.Result: Only 3 (7%) out of 42 ductal breast carcinoma were positive for EBV DNA and none of 18 benign breast tumor (control group) were positive for EBV DNA and all 60 specimens were negative for IE-antigen of HCMV expression. Conclusion:The results of this study failed to show any relationship between EBV and HCMV and the development of breast carcinoma in the tested specimens.

show abstract

Section: Discussionsupporting

confidence: 82%

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

Aidrous

Hussein²,

Dahawi

et al. 2019

NACS

View full text Add to dashboard Cite

show abstract

“…Besides, the detection of IE1mRNA indicates the presence of activation or reactivation of HCMV infection (Harwardt., 2016). The IE protein is detected in breast tumor tissue (Harkins et al, 2010;Taher et al, 2013;Mohammadizadeh et al, 2017). However, the association and expression of US28, pp65 and IEI genes were not evaluated in the present study, which requires further investigations.…”

Section: Discussionmentioning

confidence: 74%

Human Cytomegalovirus DNA among Women with Breast Cancer

Sepahvand

Makvandi

Samarbafzadeh

et al. 2019

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Section TitleBreast cancer is the most common cause of death among women worldwide. Although there are many known risk factors in breast cancer development, infectious diseases have appeared as one of the important key to contribute to carcinogenesis formation. The effects of Human Cytomegalovirus (HCMV) on women with breast cancer has been recently studied and reported. To contribute to this research trend, this study was conducted to evaluate the association between HCMV and the women with breast cancer.Objective:This experiment aimed to evaluate HCMV DNA in women with breast cancer in Ahvaz city, Iran. Materials and Methods:A total of 37 formalin fixed paraffin embedded tissues of the patients with ductal breast carcinoma and 35 paraffin embedded tissues of the patients with fibro adenoma as control group were collected. The deparaffinization of all the samples were carried out and the DNA was extracted. Initially, the PCR test was carried out to detect beta –globulin DNA as an internal control. For those samples positive for beta –globulin DNA, Polymerase Chain reaction (PCR) was used to detect HCMV for the tests and control samples. Results:Among 37 ductal breast carcinoma, 20 (54.04%) cases were proved positive for HCMV DNA by PCR. While among the 35 control group (fibroadenoma), 10 (28.57%) cases were positive for HCMV DNA (P >0.028). The prevalences of HCMV DNA among the age groups 30-39, 40-49 and >50 years were 7 (72.22%), 9 (69.23%), 4 (57.14%), respectively (P=0.066). A high frequency of HCMV DNA was detected in tumor grade III, 13/18 (58.33%) compared with tumor grade II, 7/19 (36.84%) (p=0.044). A high frequency of 16/24 (66.66%) of HCMV DNA was found in invasive ductal breast cancer compared with 4/13 (30.76%) HCMV DNA in situ (P<0.028). Conclusion:A high prevalence of 54.05% HCMV was found among the patients with ductal carcinoma. The percentages of the high prevalence of HCMV among age group (40-49) years, tumors grades, and invasive stage were (69.23%), (58.33%), (66.66%), respectively. Further study of HCMV in the latency phase in patients with ductal carcinoma would be necessary to extend our knowledge.

show abstract

“…A positive result in immunohistochemistry indicates the presence of CMV [138]. In the MENA region, this method was only used in studies of tissue biopsies of invasive CMV infections in the case of cancer [139][140][141][142]. This usage is in line with the international consensus for the diagnosis of CMV in invasive tissue disease [138].…”

Section: Laboratory Diagnosis Of CMV In the Mena Regionmentioning

confidence: 99%

The Current Status of Cytomegalovirus (CMV) Prevalence in the MENA Region: A Systematic Review

et al. 2019

View full text Add to dashboard Cite

Human cytomegalovirus (CMV) is a highly prevalent herpesvirus worldwide. According to the Centers for Disease Control and Prevention (CDC) and the World Health Organization (WHO), CMV infects people of all ages, and by the age of five, approximately one-third of children in the United States are infected. Although the infection is generally asymptomatic, it can cause severe disease in immunocompromised patients, transplant and transfusion recipients, as well as newborn neonates. The objective of this study is to systematically review published literature on CMV in the MENA region to estimate its incidence in the region and describe its epidemiological and clinical significance. The literature was searched through four scientific databases: PubMed, Scopus, Science Direct, and Web of Science. A total of 72 studies from 11 countries satisfied the inclusion criteria, covering a period from 1988–2019. The CMV IgG seroprevalence ranged from 8.7%–99.2% (SD = 38.95%). CMV incidence in these countries ranged between 1.22% and 77% in transplant and transfusion recipients, with an increase in incidence with advanced age. However, the incidence rate was unclear for congenital CMV due to the variability of the reporting. This review highlights the need for more robust and well-designed studies to better estimate CMV incidence in the MENA region, standardize diagnostic criteria, and consider prophylactic and pre-emptive treatments to limit the morbidity and mortality of the disease.

show abstract

Evaluation of human cytomegalovirus antigen expression in invasive breast carcinoma in a population of Iranian patients

Cited by 13 publications

References 22 publications

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

Human Cytomegalovirus DNA among Women with Breast Cancer

The Current Status of Cytomegalovirus (CMV) Prevalence in the MENA Region: A Systematic Review

Contact Info

Product

Resources

About