Evaluation of HPV Molecular Tests in  Primary Screening for Cervical Cancer in Brazil

Levi, José Eduardo; Longatto‐Filho, Adhemar; Eluf‐Neto, José; Rodrigues, Célia L.; Oliveira, Cristina Mendes de; Carloni, Adriana Cruvinel; Lorenzi, Adriana Tarlá; Tacla, Mauren Teresa Grubisich Mendes; Fregnani, José Humberto Tavares Guerreiro; Ab’Saber, Alexandre Muxfeldt; Scapulatempo, Cristovam; Villa, Luísa Lina

doi:10.4236/ojog.2014.48068

Cited by 11 publications

(16 citation statements)

References 32 publications

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“…Carcinogenic potential is not evenly distributed among HPVs, and HPV16 alone is responsible for more than 50% of the invasive cervical cancer (ICC) cases worldwide Li et al, 2011), and is the most prevalent HPV in other squamous carcinomas associated to HPVs infections (De Vuyst et al, 2009;de Sanjose et al, 2014), as well as in women with normal cytology (Bruni et al, 2010). In South-East Brazil, 2.3% of women on the screening population were positive for HPV16 (Levi et al, 2014), whereas 75% of the ICC cases from the same region were associated with this HPV (de Oliveira et al, 2013). Genetic diversity among HPV16 isolates can reach 2%, and the trend is towards full-genome sequencing of the isolates (Chen et al, 2005(Chen et al, , 2009Smith et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

Oliveira

Bravo

Souza

et al. 2015

Infection, Genetics and Evolution

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Invasive cervical cancer (ICC) is the third most frequent cancer among women worldwide and is associated with persistent infection by carcinogenic human papillomaviruses (HPVs). The combination of large populations of viral progeny and decades of sustained infection may allow for the generation of intra-patient diversity, in spite of the assumedly low mutation rates of PVs. While the natural history of chronic HPVs infections has been comprehensively described, within-host viral diversity remains largely unexplored. In this study we have applied next generation sequencing to the analysis of intra-host genetic diversity in ten ICC and one condyloma cases associated to single HPV16 infection. We retrieved from all cases near full-length genomic sequences. All samples analyzed contained polymorphic sites, ranging from 3 to 125 polymorphic positions per genome, and the median probability of a viral genome picked at random to be identical to the consensus sequence in the lesion was only 40%. We have also identified two independent putative duplication events in two samples, spanning the L2 and the L1 gene, respectively. Finally, we have identified with good support a chimera of human and viral DNA. We propose that viral diversity generated during HPVs chronic infection may be fueled by innate and adaptive immune pressures. Further research will be needed to understand the dynamics of viral DNA variability, differentially in benign and malignant lesions, as well as in tissues with differential intensity of immune surveillance. Finally, the impact of intralesion viral diversity on the long-term oncogenic potential may deserve closer attention.

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Section: Introductionmentioning

confidence: 99%

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

Oliveira

Bravo

Souza

et al. 2015

Infection, Genetics and Evolution

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“…HPV-DNA tests were not used in all cases. Although we used 2 HPV detection tests, a report pointed out that sensitivity and specificity was similar among them [28] . A better study design, such as a prospective model, is essential to confirm our findings.…”

Section: Discussionmentioning

confidence: 99%

Atypical Squamous Cells in Liquid-Based Cervical Cytology: Microbiology, Inflammatory Infiltrate, and Human Papillomavirus-DNA Testing

Oliveira

Eleutério²,

Gonçalves³

et al. 2017

Acta Cytologica

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Objective: The aim of this study was to assess the correlation between atypical squamous cells (ASC) and inflammatory infiltrate and vaginal microbiota using cervical liquid-based cytological (SurePath®) and high-risk human papillomavirus (HR-HPV) tests. Study Design: A cross-sectional study was conducted using a 6-year database from a laboratory in Fortaleza (Brazil). Files from 1,346 ASC cases were divided into subgroups and results concerning inflammation and vaginal microorganisms diagnosed by cytology were compared with HR-HPV test results. Results: An absence of specific microorganisms (ASM) was the most frequent finding (ASC of undetermined significance, ASC-US = 74%; ASC - cannot exclude high-grade squamous intraepithelial lesion, ASC-H = 68%), followed by bacterial vaginosis (ASC-US = 20%; ASC- H = 25%) and Candida spp. (ASC-US = 6%; ASC-H = 5%). Leukocyte infiltrate was present in 71% of ASC-US and 85% of ASC-H (p = 0.0040), and in these specific cases HR-HPV tests were positive for 65 and 64%, respectively. A positive HR-HPV test was relatively more frequent when a specific microorganism was present, and Candida spp. was associated with HR-HPV-positive results (p = 0.0156), while an ASM was associated with negative HR-HPV results (p = 0.0370). Conclusion: ASC-US is associated with an absence of inflammation or vaginosis, while ASC-H smears are associated with Trichomonas vaginalis and inflammatory infiltrate. A positive HR-HPV is associated with Candida spp. in ASC cytology.

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“…Detection of HPV DNA was performed using the Cobas1 4800 HPV platform (Roche, Indianapolis, IN, USA) which is a multiplex real-time PCR assay that provides specific genotyping information for HPV16 and 18, while concurrently detecting 12 other high-risk types (HPV 31,33,35,39,45,51,52,56,58,59, 66 and 68) in a pooled result. Vaginal self-collected and the physician-collected samples were performed according to the manufacturer's instructions.…”

Section: Hpv Testsmentioning

confidence: 99%

Detection of HPV E6 oncoprotein from urine via a novel immunochromatographic assay

et al. 2020

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Cervical cancer is a significant public health problem, especially in low-and middle-income countries, where women have little access to cervical cancer screening; consequently 80% of cervical cancer related mortality occurs in these regions. The development of screening methods that need less infrastructure thus represents an urgent medical need. The study aims to compare the detection rates of high-risk human papillomavirus 16 and 18 E6 oncoprotein in urine, vaginal self-collected, and cervical scrapes of women using the OncoE6™ Cervical Test and compare the HPV16 and/or HPV18 E6 detection rates with the HPV DNA testing. Paired urine, vaginal self-collected and cervical specimens were collected from 124 women who participated in cervical cancer screening or treatment in this proof-of-concept study and underwent to HPV16/18-E6 testing and high-risk HPV DNA testing prior to treatment of cervical neoplasia or cancer. Concordance between urinary, vaginal and cervical HPV16/18-E6 and HPV-DNA testing was evaluated for patients classified as negative group (

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Evaluation of HPV Molecular Tests in Primary Screening for Cervical Cancer in Brazil

Cited by 11 publications

References 32 publications

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

Atypical Squamous Cells in Liquid-Based Cervical Cytology: Microbiology, Inflammatory Infiltrate, and Human Papillomavirus-DNA Testing

Detection of HPV E6 oncoprotein from urine via a novel immunochromatographic assay

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