Evaluation of hepatic antioxidant changes in ovine dicrocoeliosis

Bahrami, Somayeh; Jalali, Mohammad Hossein Razi; Jafari, Arash

doi:10.1007/s12639-013-0415-1

Cited by 8 publications

(10 citation statements)

References 17 publications

(14 reference statements)

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“…For example, using samples derived from blood or tissues, antioxidant biomarkers (such as Glutathione (GSH)) could be analyzed. The GSH content indicates the cell’s defense against ROS causing cellular injury [ 123 , 124 ] and reflecting the ability of a tissue to scavenge excess superoxide anions leading to oxidative stress [ 125 ], Malondialdehyde (MDA)-a direct indicator of cell membrane damage occurring in the tissue [ 126 , 127 ], Catalase (CAT)-an indicator of CH toleration by that particular tissue [ 128 ], Superoxide dismutase (SOD)-an indicator of the active enzyme involvement in neutralizing the effect of free radicals [ 129 ] and Level of protein content-an indicator of cell capacity of mitigating the effect of free radical and peroxide processes resulting in modulating the cellular antioxidant status [ 130 ]. Furthermore, other aspects, as below, are also concerned as described below [ 131 , 132 ].…”

Section: Discussionmentioning

confidence: 99%

Citrus hystrix Extracts Protect Human Neuronal Cells against High Glucose-Induced Senescence

Pattarachotanant

Tencomnao

2020

Pharmaceuticals

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Citrus hystrix (CH) is a beneficial plant utilized in traditional folk medicine to relieve various health ailments. The antisenescent mechanisms of CH extracts were investigated using human neuroblastoma cells (SH-SY5Y). Phytochemical contents and antioxidant activities of CH extracts were analyzed using a gas chromatograph–mass spectrometer (GC-MS), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay. Effects of CH extracts on high glucose-induced cytotoxicity, reactive oxygen species (ROS) generation, cell cycle arrest and cell cycle-associated proteins were assessed using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium (MTT) assay, non-fluorescent 2′, 7′-dichloro-dihydrofluorescein diacetate (H2DCFDA) assay, flow cytometer and Western blot. The extracts protected neuronal senescence by inhibiting ROS generation. CH extracts induced cell cycle progression by releasing senescent cells from the G1 phase arrest. As the Western blot confirmed, the mechanism involved in cell cycle progression was associated with the downregulation of cyclin D1, phospho-cell division cycle 2 (pcdc2) and phospho-Retinoblastoma (pRb) proteins. Furthermore, the Western blot showed that extracts increased Surtuin 1 (SIRT1) expression by increasing the phosphorylation of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Collectively, CH extracts could protect high glucose-induced human neuronal senescence by inducing cell cycle progression and up-regulation of SIRT1, thus leading to the improvement of the neuronal cell functions.

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Section: Discussionmentioning

confidence: 99%

Citrus hystrix Extracts Protect Human Neuronal Cells against High Glucose-Induced Senescence

Pattarachotanant

Tencomnao

2020

Pharmaceuticals

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“…Oxidative damage results in structural alteration in membrane release of cell and organelle contents and essential fatty acids loss, which is seen with cytosolic aldehyde and peroxide product formation. The end product of free radical reactions on membrane fatty acids is called MDA (36). Free radicals have been reported to play a role in the pathogenesis of parasitic infections (10,11,36).…”

Section: Discussionmentioning

confidence: 99%

“…The end product of free radical reactions on membrane fatty acids is called MDA (36). Free radicals have been reported to play a role in the pathogenesis of parasitic infections (10,11,36). Many studies to date have reported increased blood concentrations of lipid peroxidation products during parasitic infections (14)(15)(16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

“…The results of this study were in agreement with those reported by Şimşek et al (16) and Samadieh et al (19), who showed increased serum MDA concentration in sheep infected with D. dendriticum. An increase of MDA concentration showed that D. dendriticum infection evokes significant changes in the oxidative status of infected hosts (36). Some studies showed that serum MDA levels were increased in sheep (33) and cattle (37) with liver cystic echinococcosis.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of biochemical parameters and oxidative stress in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts

Çınar¹,

Aydenizöz²,

Gökpınar³

et al. 2018

Turk J Vet Anim Sci

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Introduction Parasitic diseases caused by liver trematodes have an important place among many parasitic infections causing reduced productivity in animals. Liver trematodes constitute an important parasitic group affecting domestic ruminants around the world and in Turkey. Animals infected by this parasite manifest symptoms such as deterioration of overall status, edema, anemia, and weakness. It has been reported that in infected animals the bile ducts are dilated, the gall bladder is filled with bile, and the liver becomes fibrotic and cirrhotic and thus stiffer and hard to dissect over time (1). Among liver trematodes, D. dendriticum settles in the liver of ruminants and causes weight loss and reduced milk yield (2). Another parasite causing hepatic injury in sheep is the hydatid cyst, which is the larval form of Echinococcus granulosus. After ingestion of food material contaminated by canine feces, hydatid cysts primarily infect ruminants, but also camels, rabbits, pigs, humans, and monkeys. It primarily involves the liver, but also the lungs, spleen, and heart, and rarely other organs. Since its larvae involve the liver and lungs of intermediary hosts, they do not pose many problems when young. When they are fully grown, however, they cause jaundice if they reside in the liver. Hepatic dysfunction leads to digestive disorders, while cysts in other organ systems give rise to organ-specific disorders (3). Biochemical changes in blood are used to diagnose many disorders (4). Important changes can occur in the serum biochemistry of animals during parasitic infections (5,6). These infections are held responsible for hypoproteinemia and reduced levels of trace elements due mainly to malabsorption and increase in liver enzymes due to resulting hepatic injury (5,7). Dağoğlu et al. (8) reported that tissue injury induced by parasites involving the liver, such as D. dendriticum, caused altered serum enzymatic activity, which is particularly important for early detection of toxic hepatic injury. Minerals have an important role in maintaining normal physiological functions and protecting organisms against disease. These substances are found in the structure of cofactors that are necessary for enzymatic activity. Copper (Cu) is an element that must be supplied in the diet of domestic livestock. Its deficiency is characterized by oxidation disturbance, anemia, hair depigmentation,

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“…A significant MDA decrease was observed due to lipid peroxidation which is a direct indicator that cell membrane damage has occurred in the tissue (Jonas et al, 2000;AshokKumar, 2004). Increased CAT observed in this study may indicate enhanced triherbal toleration by that particular tissue (Bahrami et al, 2015). Rise in SOD activities observed may indicate presence of active enzyme involvement in neutralizing the effect of free radicals (Deger et al, 2008).…”

Section: Discussionmentioning

confidence: 56%

Evaluation Of Biochemical, Hematological And Antioxidant Properties In Mice Exposed To A Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular

Kehinde

Adebayo

Adesiyan

et al. 2020

Preprint

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Nigella sativa, Carica papaya and Boswellia sacra are medicinal plants in the commonly used in folkloric medicine due to the presence of its immense therapeutic properties. Fifty (50) female albino mice weighing between 15-22g were divided into five groups of 10 mice each. Animal in group 1 served as control group and were administered distilled water while animal in group 2 were given 2ml of cisplatin (orally). Animal in group 3-5 were given orally; 100 mg/kg (low dose), 200 mg/kg (medium dose) and 400 mg/kg (high dose) of triherbal preparation. The feeding regimens lasted for 28 days. After 28 days, mammary gland and blood samples were collected for haematological and antioxidant analysis. The triherbal formula decreased the GSH and MDA levels of mice treated with 100 mg/kg and 400 mg/kg doses compare to control. The measurement of total protein content, SOD and CAT increased in treated animals compared to control. However, RBC (Red Blood Cell) counts significantly decreased in the low, medium and high dose groups (0.95 ±0.08, 6.57 ±0.08 and 3.55 ±0.55 x 106 cells/mm3 respectively) compared to control (7.34 ±0.40) at P<0.05. Also, significant decreases (P<0.05) in the level of the total WBC (White Blood Cell) count, platelet count, PCV (Packed Cell Volume) and Hb (haemoglobin) concentration were observed. The decreases were dose dependent. The MCH (Mean Corpuscular Haemoglobin) and MCHC (Mean Corpuscular Haemoglobin Concentration) except MCV (Mean Corpuscular Volume) significantly decreased in treated group only. The triherbal formulation exhibited significant antioxidant activities showing increased levels of SOD, CAT and Protein content due to activation of the enzyme involve in detoxification of free radicals and decreased in the level of GSH and MDA due to accumulation of peroxides and H2O2. Also, decreased in haematological parameters due to the presence of phytochemicals such as phenol, resins, saponins, sterols, tannis and terpenes in the triherbal formula. Therefore, it has potential to induce haematotoxicity hence consumption of high concentrations should be discouraged.

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Evaluation of hepatic antioxidant changes in ovine dicrocoeliosis

Cited by 8 publications

References 17 publications

Citrus hystrix Extracts Protect Human Neuronal Cells against High Glucose-Induced Senescence

Citrus hystrix Extracts Protect Human Neuronal Cells against High Glucose-Induced Senescence

Evaluation of biochemical parameters and oxidative stress in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts

Evaluation Of Biochemical, Hematological And Antioxidant Properties In Mice Exposed To A Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular

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