Evaluation of ‘Hamlin’ sweet orange+‘Montenegrina’ mandarin somatic hybrid for tolerance to Xanthomonas axonopodis pv. citri and Xylella fastidiosa

Pavan, Alexandra; Calixto, Marcia Cristina; Cardoso, Suely Campos; Mendes, Beatriz Madalena Januzzi; Filho, Armando Bergamin; Lopes, João Roberto Spotti; Carvalho, Carlos Roberto; Filho, Francisco de Assis Alves Mourão

doi:10.1016/j.scienta.2007.03.022

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…Among these tools, somatic hybridization has been applied to produce disease-tolerant rootstock (Ananthakrishnan et al 2006;Mendes et al 2001;Costa et al 2003;Calixto et al 2004;Grosser et al 2000;Mourão Filho et al 2008) and scion cultivars (Pavan et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Experimental plants were individually spray-inoculated on both sides of the young leaves with a 10 6 cfu mL −1 Xcc suspension and covered with a clear plastic bag for 72 h in order to keep high humidity (Dalla Pria et al 2006;Pavan et al 2007). Plants were then transferred to a growth chamber (Conviron® model PGW 36, Winnipeg, Canada) with restricted access due to biosafety regulations, in a 12-h photoperiod, at 28°C.…”

Section: Propagation Of Transgenic Plants and Evaluation For Resistanmentioning

confidence: 99%

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Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

et al. 2009

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Genetic transformation with genes that code for antimicrobial peptides has been an important strategy used to control bacterial diseases in fruit crops, including apples, pears, and citrus. Asian citrus canker (ACC) caused by Xanthomonas citri subsp. citri Schaad et al. (Xcc) is a very destructive disease, which affects the citrus industry in most citrus-producing areas of the world. Here, we report the production of genetically transformed Natal, Pera, and Valencia sweet orange cultivars (Citrus sinensis L. Osbeck) with the insect-derived attacin A (attA) gene and the evaluation of the transgenic plants for resistance to Xcc. Agrobacterium tumefaciens Smith and Towns-mediated genetic transformation experiments involving these cultivars led to the regeneration of 23 different lines. Genetically transformed plants were identified by polymerase chain reaction, and transgene integration was confirmed by Southern blot analyses. Transcription of attA gene was detected by Northern blot analysis in all plants, except for one Natal sweet orange transformation event. Transgenic lines were multiplied by grafting onto Rangpur lime rootstock plants (Citrus limonia Osbeck) and sprayinoculated with an Xcc suspension (10 6 cfu mL −1 ). Experiments were repeated three times in a completely randomized design with seven to ten replicates. Disease severity was determined in all transgenic lines and in the control (nontransgenic) plants 30 days after inoculation. Four transgenic lines of Valencia sweet orange showed a significant reduction in disease severity caused by Xcc. These reductions ranged from 58.3% to 77.8%, corresponding to only 0.16-0.30% of leaf diseased area as opposed to 0.72% on control plants. One transgenic line of Natal sweet orange was significantly more resistant to Xcc, with a reduction of 45.2% comparing to the control plants, with only 0.14% of leaf diseased area. Genetically transformed Pera sweet orange plants expressing attA gene did not show a significant enhanced resistance to Xcc, probably due to its genetic background, which is naturally more resistant to this pathogen. The potential effect of attacin A antimicrobial peptide to control ACC may be related to the genetic background of each sweet orange cultivar regarding their natural resistance to the pathogen.

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Section: Discussionmentioning

confidence: 99%

Section: Propagation Of Transgenic Plants and Evaluation For Resistanmentioning

confidence: 99%

Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

et al. 2009

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“…This cultivar is a typical willowleaf or Mediterranean mandarin which is very popular in Brazil and is moderately tolerant to citrus canker caused by the bacterium Xanthomonas citri subsp. citri (PAVAN et al, 2007;OLIVEIRA et al, 2016). Fruits are small to medium in size, seedy, easy to peel, and transport resistant, showing excellent quality with a unique flavor (PANZENHAGEN et al, 1999;PAVAN et al, 2007;KOLLER, 2009;BRACKMANN et al, 2008;ROSA et al, 2012;NEVES et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…citri (PAVAN et al, 2007;OLIVEIRA et al, 2016). Fruits are small to medium in size, seedy, easy to peel, and transport resistant, showing excellent quality with a unique flavor (PANZENHAGEN et al, 1999;PAVAN et al, 2007;KOLLER, 2009;BRACKMANN et al, 2008;ROSA et al, 2012;NEVES et al, 2018). Therefore, this study evaluated the horticultural performance of 'Montenegrina' mandarin trees grafted on multiple rootstocks in Londrina, Paraná, Brazil.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of multiple rootstocks for ‘Montenegrina’ mandarin in Londrina, Paraná, Brazil

et al. 2022

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Most commercial orchards of mandarins in Paraná grow ‘Ponkan’ trees predominantly grafted on ‘Rangpur’ lime. The limited genetic diversity of citrus orchards for both scion and rootstock may promote pest and disease outbreaks. The late season ‘Montenegrina’ willowleaf mandarin has high potential for orchard diversification because of its higher productivity and better fruit quality. Therefore, this study assessed the agronomic performance of ‘Montenegrina’ mandarin trees grafted on multiple rootstocks under the edaphoclimatic conditions of Londrina, Paraná, Brazil. The trial was set in a randomized block design using the rootstocks of ‘Rangpur’ lime, ‘Cleopatra’ mandarin, ‘Sunki’ mandarin, ‘Swingle’ citrumelo, and ‘Fepagro C-13’ citrange replicated six times. The ‘Montenegrina’ mandarin trees were assessed for vegetative growth, yield, and fruit quality. The trees had similar size and yield, regardless of the rootstock, and low alternate bearing indices. The fruit quality parameters varied among the rootstocks between the four cropping seasons. Fruits from trees on ‘Rangpur’ and ‘Cleopatra’ exhibited the highest maturation indices, whereas those from ‘Swingle’ and ‘Fepagro C-13’ produced larger fruits with low number of seeds. In general, ‘Montenegrina’ trees can be grown on all evaluated rootstocks without incompatibility and loses in Londrina, Northern Paraná, Brazil.

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“…For 'Hamlin' sweet orange ? 'Montenegrina' mandarin, regenerated allotetraploid somatic hybrids showed improved tolerance to ACC (Pavan et al 2007). In this study, recovered allotetraploid somatic hybrids between 'Bingtang' orange and Calamondin are expected to be useful material for ACC-tolerant citrus breeding, and further evaluation of tolerance of these hybrids to ACC is needed.…”

Section: Molecular Identification Of Regenerated Plantsmentioning

confidence: 90%

Production and molecular characterization of two new Citrus somatic hybrids for scion improvement

Cai

Duan

et al. 2009

Acta Physiol Plant

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Somatic hybridization by protoplast fusion from cell suspension cultures and leaf parent has been a well-established technique holding great potential for citrus variety improvement. In this study, somatic hybrid plants were regenerated from the following two fusion combinations: 'Murcott' tangor (Citrus reticulata Blanco 9 C. sinensis (L.) Osbeck) ? Hirado Buntan Pink pummelo (HBP) (C. grandis (L.) Osbeck) and 'Bingtang' orange (C. sinensis (L.) Osbeck) ? Calamondin (C. microcarpa Bunge). Somatic hybrids were selected at an early stage based on their higher capacity for embryogenesis comparing to non-hybrid cells. Flow cytometry analysis showed that all plants from pre-selected lines of the two combinations were tetraploid. SSR analysis confirmed their hybrid nature, with nuclear DNA from both fusion parents, and absence of parental specific bands was also detected. Cytoplasmic compositions of the recovered plants were further revealed by CAPS and cpSSR analysis. The allotetraploid somatic hybrids from the 'Murcott' tangor ? HBP combination will be applied to develop triploid seedless cultivars by interploid crossing with diploid seedy citrus cultivars, and those from 'Bingtang' orange ? Calamondin could be valuable for Asiatic citrus canker-tolerant and ornamental citrus breeding.

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Evaluation of ‘Hamlin’ sweet orange+‘Montenegrina’ mandarin somatic hybrid for tolerance to Xanthomonas axonopodis pv. citri and Xylella fastidiosa

Cited by 10 publications

References 33 publications

Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

Transgenic Sweet Orange (Citrus sinensis L. Osbeck) Expressing the attacin A Gene for Resistance to Xanthomonas citri subsp. citri

Evaluation of multiple rootstocks for ‘Montenegrina’ mandarin in Londrina, Paraná, Brazil

Production and molecular characterization of two new Citrus somatic hybrids for scion improvement

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