Evaluation of gold nanoparticles toxicity towards human endothelial cells under static and flow conditions

Fede, Caterina; Fortunati, Ilaria; Weber, Verena; Rossetto, Nicola; Bertasi, Federico; Petrelli, Lucía; Guidolin, Diego; Signorini, Raffaella; De, Raffaele; Albertin, Giovanna; Ferrante, Camilla

doi:10.1016/j.mvr.2014.10.010

Cited by 66 publications

(59 citation statements)

References 46 publications

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“…Collectively, these features may negatively affect endothelial cell growth and viability at concentrations of 100 μg/ml and higher. Moreover, the recently reported data as well as our present studies indicate that physiologic flow is one of the important factors that must be considered when designing drug delivery nanosystems, as the internalization of untargeted nanoparticles by endothelial cells differs greatly between static and dynamic conditions [34][35][36].…”

Section: Io-np2supporting

confidence: 58%

“…Whereas laminar flow protects endothelial cells from harmful stimuli, nonuniform shear stress induces endothelial activation [31]. Furthermore, recent studies showed that the endothelial uptake on untargeted nanoparticles greatly depends on the presence and magnitude of shear stress [34][35][36]. Therefore, experiments under flow conditions are necessary to estimate the cell responses in physiological-like settings.…”

Section: Nanoparticle Effects On Ecs In Dynamic Cell Culture Conditionsmentioning

confidence: 99%

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Nanoparticles for Intravascular Applications: Physicochemical Characterization and Cytotoxicity Testing

Matuszak

Baumgartner

Zaloga

et al. 2016

Nanomedicine (Lond.)

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Aim:We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.

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Section: Io-np2supporting

confidence: 58%

Section: Nanoparticle Effects On Ecs In Dynamic Cell Culture Conditionsmentioning

confidence: 99%

Nanoparticles for Intravascular Applications: Physicochemical Characterization and Cytotoxicity Testing

Matuszak

Baumgartner

Zaloga

et al. 2016

Nanomedicine (Lond.)

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“…This is also in agreement with the data previously published by Fede et al who detected the same tendency for the internalization of AuNPs (w/o PEG) in HUVEC under static and flow conditions using a microfluidic system. 36 In addition to shear stress, cyclic stretch is also a prominent hemodynamic force which acts on endothelial cell function and membrane traffic 17,37 and may alter the internalization of nanoparticles in endothelial cells. As previously reported, we showed that stretch affects the interaction of endothelial cells with amorphous silica nanoparticles and leads to a reduced internalization.…”

Section: Resultsmentioning

confidence: 99%

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions

et al. 2017

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a PEGylated gold nanoparticles (AuNPs) have an extended circulation time after intravenous injection in vivo and exhibit favorable properties for biosensing, diagnostic imaging, and cancer treatment. No impact of PEGylated AuNPs on the barrier forming properties of endothelial cells (ECs) has been reported, but recent studies demonstrated that unexpected effects on erythrocytes are observed. Almost all studies to date have been with static-cultured ECs. Herein, ECs maintained under physiological cyclic stretch and flow conditions and used to generate a blood-brain barrier model were exposed to 20 nm PEGylated AuNPs. An evaluation of toxic effects, cell stress, the release profile of pro-inflammatory cytokines, and blood-brain barrier properties showed that even under physiological conditions no obvious effects of PEGylated AuNPs on ECs were observed. These findings suggest that 20 nm-sized, PEGylated AuNPs may be a useful tool for biomedical applications, as they do not affect the normal function of healthy ECs after entering the blood stream.

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“…A recent study by Fede (2014) evaluated the toxicity of gold nanoparticles to human endothelial cells in both static and dynamic flow conditions of exposure (Fede, Fortunati et al 2015). They confirmed that at comparable concentrations, in static conditions the toxicity of nanoparticles is higher.…”

Section: Introductionmentioning

confidence: 88%

Effectiveness of Protective Gloves against Engineered Nanoparticles: Difficulties in Evaluation

Vinches¹,

Zemzem²,

Hallé³

et al. 2016

IJTAN

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Evaluation of gold nanoparticles toxicity towards human endothelial cells under static and flow conditions

Cited by 66 publications

References 46 publications

Nanoparticles for Intravascular Applications: Physicochemical Characterization and Cytotoxicity Testing

Nanoparticles for Intravascular Applications: Physicochemical Characterization and Cytotoxicity Testing

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions

Effectiveness of Protective Gloves against Engineered Nanoparticles: Difficulties in Evaluation

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