Evaluation of GenoType and LiPA MYCOBACTERIA Assays for Identification of Finnish Mycobacterial Isolates

Mäkinen, Johanna; Sarkola, Aleksi; Marjamäki, Merja; Viljanen, Matti K.; Soini, Hanna

doi:10.1128/jcm.40.9.3478-3481.2002

Cited by 44 publications

(29 citation statements)

References 25 publications

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“…Molecular methods have replaced conventional biochemical tests for the identification of NTM. With the use of PCR-RFLP, DNA probe technology has broad commercial availability, including products from AccuProbe (Gen-Probe, San Diego, CA), INNO-LiPA (Fujirebio, Ghent, Belgium), GenoType (Hain Lifescience GmbH, Nehren, Germany), and REBA Myco-ID (YD Diagnostics, Yongin, Republic of Korea), and is one of the most widely used methods in many diagnostic laboratories (16,27). The different detection ranges of commercial assays are illustrated in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, various genotype-based methods for the identification of NTM have been developed to rapidly identify mycobacteria, including commercially available molecular probes, PCR-restriction fragment length polymorphism (RFLP), and sequencing (16)(17)(18). However, determining an accurate distribution of NTM can still be problematic due to the different detectable ranges and sensitivities of different methods.…”

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confidence: 99%

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Distribution of Nontuberculous Mycobacteria by Multigene Sequence-Based Typing and Clinical Significance of Isolated Strains

et al. 2014

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bSpecies identification of nontuberculous mycobacteria (NTM) is challenging due to the increasing number of identified NTM species and the lack of standardized testing strategies. The objectives of this study were to investigate the distribution of NTM species recovered from respiratory specimens by multigene sequence-based typing and to evaluate the clinical significance of identified species. Two hundred thirty-two consecutive clinical NTM isolates were subjected to sequencing of multiple genes, including hsp65, rpoB, and 16S-23S rRNA internal transcribed spacer (ITS) sequence. In addition, clinical data from all patients whose specimens had NTM isolates were analyzed to examine clinical virulence and treatment history. Eighteen strains from 227 isolates from 169 patients were successfully identified at the species level by multigene sequence-based typing. Mycobacterium avium complex and M. abscessus complex made up the majority of isolated NTM (88%; 199/227), followed by M. fortuitum complex (4%; 10/227). The pathogenic potential of NTM differs enormously by species, and M. avium complex and M. abscessus complex revealed especially high levels of virulence compared with findings for other NTM species. The results from our work support M. avium complex and M. abscessus complex being the most common NTM species with highly pathogenic potential isolated from clinical respiratory specimens and could be a good resource for molecular epidemiology of NTM species in South Korea.A ll members of the genus Mycobacterium, excepting M. tuberculosis and M. leprae, are considered nontuberculous mycobacteria (NTM) and are frequently isolated from environmental sources, including surface or tap water and soil (1). Currently, more than 160 mycobacterial species/subspecies are listed in the Genus Mycobacterium database (http://www.bacterio.cict.fr/m /mycobacterium.html; accessed on 22 August 2013), and the number of newly identified NTM is increasing. Recent data suggest that the incidence of lung disease caused by NTM with or without predisposing risk factors has been growing worldwide (2-6). However, epidemiologic data on the distribution and clinical significance of NTM lung disease are still scarce because NTM lung disease is not a reportable condition in most countries.In South Korea, NTM was first described as a cause of mycobacterial lung disease in 1981 (7); since then, the frequency of isolation of NTM from clinical specimens has shown a continuous increase. Since the 2000s, the frequency of NTM isolation has been reported to be 12 to 38% of mycobacterial culture-positive specimens, and the number of patients with NTM disease has also been considerable, with a frequency of 8 to 49% of isolated NTM (8-15). The isolation of NTM species from respiratory specimens is not sufficient evidence for the diagnosis of clinically significant NTM lung disease, since there are also clinical, radiographic, and microbiological criteria (1).Recently, various genotype-based methods for the identification of NTM have been developed to ra...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Distribution of Nontuberculous Mycobacteria by Multigene Sequence-Based Typing and Clinical Significance of Isolated Strains

et al. 2014

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“…The specificity of the M. tuberculosis complexspecific 23S rDNA fragment of this test is well known from the performance of the GenoType Mycobacteria (Hain Lifescience) test, which is based on the same fragment (13).…”

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confidence: 99%

Usefulness of the GenoType MTBC Assay for Differentiating Species of the Mycobacterium tuberculosis Complex in Cultures Obtained from Clinical Specimens

Richter

Weizenegger²,

Fahr³

et al. 2004

J Clin Microbiol

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(17,23,24,25). Although the species are closely related genetically, they differ in host and geographic range, certain phenotypes, and pathogenicity. M. tuberculosis is the most significant pathogen for humans in Europe and America, whereas M. africanum is widely distributed among African patients (4, 15). Both subspecies of M. bovis are reported to infect humans, yet they have a broad host range, including wildlife and domestic livestock like bovines and goats (12,26). Of particular interest is the intrinsic resistance of M. bovis subsp. bovis against pyrazinamide, one of the first-line antituberculous drugs (11,17). The vaccine strain M. bovis BCG is more frequently used for bladder cancer immunotherapy and can be detected in human urine specimens from bladder cancer patients (2, 6). Thus, for those specimens, the isolation of M. tuberculosis complex is not necessarily an indication for antituberculous treatment. Therefore, rapid differentiation to the species and subspecies levels should be obtained not only for epidemiological purposes but also for adequate treatment of each patient.So far, rapid identification of the M. tuberculosis complex has been achieved by using AccuProbe gene probes (Gen-Probe, San Diego, Calif.), which target the 16S rRNA. However, species identification still relies on the determination of a series of phenotypic and growth characteristics and on biochemical tests, which all require sufficient growth, are time-consuming, and may not be performed in every laboratory (5, 25). Recently, the GenoType MTBC (Hain Lifescience GmbH, Nehren, Germany)-a new commercially available DNA strip assay for the rapid identification of the members of the M. tuberculosis complex-was evaluated by use of a well-characterized collection of M. tuberculosis complex isolates and was proven to be useful for species differentiation (20). Moreover, due to the inclusion of an M. tuberculosis complex-specific oligonucleotide, this test may replace the AccuProbe tests of cultures from clinical specimens as a method for identification of the M. tuberculosis complex, giving the additional information provided for the species involved.The aim of this work was to evaluate the performance of the GenoType MTBC with positive liquid cultures from clinical specimens received for mycobacterial culture in comparison to species identification by classical methods. Furthermore, the GenoType MTBC results were compared to results obtained by AccuProbe tests.Specimens. Two German microbiology laboratories were involved in this study: (i) the National Reference Center for Mycobacteria, Borstel, Germany, and (ii) the Department of Microbiology of the Laboratory Group, Heidelberg, Germany. All samples used in this study originated from human specimens and a few veterinary specimens sent to the laboratories for routine mycobacterial culture. Specimens (pulmonary and extrapulmonary) were processed according to national and international guidelines (5, 10) by using an N-acetyl-L-cysteineNaOH decontamination procedure, inoculated into B...

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“…This format enables simultaneous detection and identification of different mycobacterial species. At present, two systems with this design are commercially available in Europe, the INNO-LiPA Mycobacteria test, designed to amplify the mycobacterial 16S-23S rRNA spacer region (8,10,11,18,(21)(22)(23), and the GenoType Mycobacteria assay, targeting the mycobacterial 23S rRNA (7). Any of these methods are available in the United States as Food and Drug Administration-approved tests and are used only for diagnosis.…”

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confidence: 99%

“…Recently, new molecular biology techniques based on the PCR and reverse hybridization procedure have been marketed for mycobacterial identification (7,8,10,11,18,(21)(22)(23). The hybridization technique is performed on nitrocellulose strips onto which probe lines are fixed in parallel.…”

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confidence: 99%

Comparative Evaluation of the New Version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains

et al. 2004

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Evaluation of GenoType and LiPA MYCOBACTERIA Assays for Identification of Finnish Mycobacterial Isolates

Cited by 44 publications

References 25 publications

Distribution of Nontuberculous Mycobacteria by Multigene Sequence-Based Typing and Clinical Significance of Isolated Strains

Distribution of Nontuberculous Mycobacteria by Multigene Sequence-Based Typing and Clinical Significance of Isolated Strains

Usefulness of the GenoType MTBC Assay for Differentiating Species of the Mycobacterium tuberculosis Complex in Cultures Obtained from Clinical Specimens

Comparative Evaluation of the New Version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains

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