Evaluation of generations 2, 3 and 4 arginine modified PAMAM dendrimers for gene delivery

Nam, Hye Yeong; Hahn, Hwa Jeong; Nam, Ki Woong; Choi, Woo-Hyung; Jeong, Yunseong; Kim, Dong-Eun; Park, Jong‐Sang

doi:10.1016/j.ijpharm.2008.07.021

Cited by 76 publications

(40 citation statements)

References 19 publications

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“…This polymer was expected to have two advantages for gene delivery: an enhanced transfection efficiency as a result of the introduction of arginine for DNA carriers, and fast biodegradation able to lower cytotoxicity and deliver antisense ODNs and small interfering RNAs to modulate gene expression. This suggested that the transfection efficiency of e-PAM-R G4 was higher than that of either G3 or G2, and was similar to PAMAM-R G4 with favorable cell viability (17).…”

Section: Heat Treatment Tang Et Almentioning

confidence: 96%

“…With each new generation from G0 to G10, the molecular weights and the numbers of active surface units of PAMAM dendrimers have increased exponentially, while their diameters have increased linearly. The toxicity is related to the generation and increases with the number of surface groups (16,17) as the size influences the cytotoxicity of the dendrimer. The increasing branch density with generation is believed to have striking effects on the structure of dendrimers which are characterized by the presence of internal cavities to encapsulate a large number of reactive guest molecules.…”

Section: Characteristics Of Pamam Dendrimersmentioning

confidence: 99%

“…Efficient transfection was not limited to neurons, but was extended to all three glial cells (astrocytes, microglia and oligodendrocytes) that were present in these primary cortical cultures. Nam et al (17) designed and synthesized a new biodegradable polymer, e-PAM-R, which was modified with arginine by esterification. This polymer was expected to have two advantages for gene delivery: an enhanced transfection efficiency as a result of the introduction of arginine for DNA carriers, and fast biodegradation able to lower cytotoxicity and deliver antisense ODNs and small interfering RNAs to modulate gene expression.…”

Section: Heat Treatment Tang Et Almentioning

confidence: 99%

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Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency

Wang

Shi

Yin

2011

Experimental and Therapeutic Medicine

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Abstract. Hair cells in the cochlea can be damaged by various insults, including noise, drugs, infections and presbycusis, which may cause sensorineural hearing loss. Gene therapy is a novel therapeutic technology that, recently, has led to the idea of treating inner ear diseases on a genetic level. Depending on their characteristics, such as a high efficiency in delivery, the capability of specific targeting, multifunctionality, biodegradability, non-toxicity, non-immunogenicity and the capability of limiting DNA degradation, nanovectors, such as polyamidoamine (PAMAM) dendrimers for cellular gene delivery, provide a promising approach to eradicate genetic diseases. They are a new class of highly branched spherical polymers that are highly soluble in aqueous solution. Their unique surface is composed of positively charged primary amine groups which allow them to form stable complexes with plasmid DNA, oligonucleotides, antibodies and drugs. This review provides an overview of the characteristics of PAMAMs which may be used in gene transfer into the cochlea as well as the efforts to improve their transfection efficiency as gene-delivery carriers.

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Section: Heat Treatment Tang Et Almentioning

confidence: 96%

Section: Characteristics Of Pamam Dendrimersmentioning

confidence: 99%

Section: Heat Treatment Tang Et Almentioning

confidence: 99%

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Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency

Wang

Shi

Yin

2011

Experimental and Therapeutic Medicine

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“…Therefore, hydroxyl-terminated PAMAM-OH G 2, 3, and 4 were modified with arginine by ester bond formation, resulting in e-PAM-R G 2, 3, and 4 dendrimers. 58 The core PAMAM-OH displayed low cell toxicity, transfection efficiency, …”

Section: à20mentioning

confidence: 99%

Nucleic Acid Carriers Based on Precise Polymer Conjugates

Troiber

Wagner

2011

Bioconjugate Chem.

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Polymer polydispersity, random conjugation of functional groups, and poorly understood structure–activity relationships have constantly hampered progress in the development of nucleic acid carriers. This review focuses on the synthetic concepts for the generation of precise polymers, site-specific conjugation strategies, and multifunctional conjugates for nucleic acid transport. Dendrimers, defined peptide carriers, sequence-defined polyamidoamines assembled by solid-phase supported synthesis, and precise lipopeptides or lipopolymers have been characterized for pDNA and siRNA delivery. Conjugation techniques such as click chemistries and peptide ligation are available for conjugating polymers with functional transport elements such as targeting or shielding domains and for direct covalent modification of therapeutic nucleic acids in a site-specific mode.

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“…5,6 PAMAM dendrimers are also widely used for both drug and gene delivery. [7][8][9] PEI is a highly efficient cationic polymer since it has numerous amine groups in its structure. However, it also shows strong cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

PAMAM Dendrimers Conjugated with L-Arginine and γ-Aminobutyric Acid as Novel Polymeric Gene Delivery Carriers

Son¹,

Yu²,

Choe³

et al. 2013

Bulletin of the Korean Chemical Society

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In this study, we synthesized functional dendrimer derivatives as nonviral gene delivery vectors. Poly(amidoamine) dendrimer (PAMAM, generation 4) was modified to possess functional amino acids to enhance gene transfection efficiency. PAMAM G4 derivatives conjugated with L-arginine (Arg) and γ-aminobutyric acid (GABA) showed higher transfection efficiency and lower cytotoxicity compared to the native PAMAM G4 dendrimer. The polyplex of the PAMAM G4 derivative/pDNA was evaluated using an agarose gel retardation assay and Picogreen reagent assay. Additionally, the MTT assay was performed to examine the cytotoxicity of synthesized polymers. All PAMAM G4 derivatives showed lower cytotoxicity than PEI25kD. Particularly, PAMAM G4-GABA-Arg displayed enhanced transfection efficiency compared to the native PAMAM G4 dendrimer.

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Evaluation of generations 2, 3 and 4 arginine modified PAMAM dendrimers for gene delivery

Cited by 76 publications

References 19 publications

Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency

Polyamidoamine dendrimers as gene delivery carriers in the inner ear: How to improve transfection efficiency

Nucleic Acid Carriers Based on Precise Polymer Conjugates

PAMAM Dendrimers Conjugated with L-Arginine and γ-Aminobutyric Acid as Novel Polymeric Gene Delivery Carriers

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