Asian seabass (Lates calcarifer, Bloch), a euryhaline fish reproduced in seawater is one of the most important aquaculture species throughout Southeast Asia and Australia due to acceptable flavor and high market demand. Annual production of L. calcarifer in Thailand was 2,503 tons in 1994 and increased to 20,454 tons, equivalent a market value of nearly 90 million US$ in 2017 (Fisheries Statistics of Thailand, 2019). This indicates an increase in a market need of L. calcarifer seed stocks for domestic and international trades. One of the major challenges to manage the breeding program of L. calcarifer held in hatcheries is the uncertainty from year to year in the number of functional male broodstocks due to the natural sex inversion from male to female. Because of a cohort reproductive behavior, several L. calcarifer males are mated with one or two females (at a ratio of 4:1, a common practice in the hatcheries of Thailand), which consequently generates a back-up supply of highly desired male broodstocks. Furthermore, stress derived from the handling was deteriorative to the quality of expressible semen of male broodstocks (Palmer et al., 1993).Chilled storage of fish semen is a simple protocol that extends sperm life from a few hours to several weeks with maintaining