Evaluation of experimental Toxoplasma gondii (Nicolle and Manceaux, 1909) infection in pigs by bioassay in mice and polymerase chain reaction

Yai, Lúcia Eiko Oishi; Vianna, Manoella Compostrini Barreto; Soares, Rodrigo Martins; Cortéz, Adriana; Freire, Roberta Lemos; Richtznhain, Leonardo José; Gennari, Solange Maria

doi:10.1590/s1413-95962003000300010

Cited by 32 publications

(24 citation statements)

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“…This may due to the fact that PCR was able to produce a linear relationship between the relative amounts of PCR product and the number of tachyzoites in order of 10 3 tachyzoites. Similar results 21.8% (7/32) were obtained by nested PCR when tissue samples of pigs challenged with oocysts were evaluated (Yai et al, 2003). Commercial meat products from the UK were evaluated for T. gondii by SAG2 amplification and the authors (Aspinall et al, 2002) found 27/71(38%) positive samples.…”

Section: Resultssupporting

confidence: 58%

“…PCR used in conditions of this experiment was able to detect the DNA of the parasite at a concentration of 10 3 tachyzoites/ml. (Yai et al, 2003). However, PCR may not express the risk of human infection (Aspinall et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

“…This method is very sensitive, highly specific and rapid (Yai et al, 2003). Mouse bioassay is the principal method used to detected cysts in tissues, but it is arduous and expensive.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Tsutsui

Freire

Garcia

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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The distribution of T. gondii in commercial cuts of pork (ham, tenderloin, spareribs and arm picnic) by PCR and bioassay from experimentally infected pigs, was evaluated. Eighteen mixed breed pigs were divided into two groups (G). The G1 animals (n=10) were infected with 4 x10 4 oocysts of the T. gondii VEG strain and the G2 animals (n=8) were used as control. Pigs of both groups were slaughtered at 59 th day after infection, and meat samples were collected for bioassay and PCR. All animals from G1 were positive by at least one or both tests, and all control animals were negative. T. gondii was identified in pork by mouse bioassay and PCR in 27/40 (67.5%) and in 9/40 (22.5%) of the evaluated samples, respectively. There were no statistical differences in the distribution of tissue cysts from commercial cuts of pork by bioassay (P>0.05). However, statistical differences were observed when mouse bioassay and PCR were compared (P<0.01). Keywords: pork meat, mouse bioassay, PCR, Toxoplasma gondii RESUMO Avaliou-se a presença de T. gondii em cortes comerciais de carne suína (pernil, lombo, costela e paleta), por meio do bioensaio e PCR, em animais experimentalmente inoculados. Dois grupos (G) foram formados. Os animais do G1 (n=10) foram inoculados com 4 x10 4 oocistos da cepa VEG e os do G2 (n=8) permaneceram como grupo-controle, não inoculado. Todos os animais foram abatidos no dia

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Section: Resultssupporting

confidence: 58%

Section: Resultsmentioning

confidence: 99%

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Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Tsutsui

Freire

Garcia

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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“…This is corroborated by Frazão-Teixeira et al (2006) who isolated T. gondii in 50.0% of pigs in the state of Rio de Janeiro. However, higher values were found by Yai et al (2003) and Tsutsui et al (2007), who found 87.5% and 67.5% of positive pigs, respectively. The latter two studies were performed on experimentally infected pigs, whereas naturally exposed pigs were examined in the present study.…”

Section: Discussionmentioning

confidence: 73%

Comparison of methods for detection of Toxoplasma gondii in tissues of naturally exposed pigs

et al. 2011

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This study aimed to compare the sensitivity of five diagnostic methods commonly used for the detection of Toxoplasma gondii in tissues of naturally infected pigs. We purchased 20 heads of pigs in butcher shops in the city of Ilhéus, Bahia. Brain and tongue fragments were taken from each animal for the performance of PCR against T. gondii. The rest of these two tissues were processed and inoculated into three mice. These rodents were observed for 42 days and euthanized. We prepared slides with brain and lungs of each mouse for the visualization of T. gondii. From the tissues of mice, we carried out polymerase chain reaction (PCR), histopathology, and immunohistochemistry in an attempt to identify the parasite. The PCR direct from the tissue of pigs showed 10% (2/20) of positive samples, all from the brain. PCR in tissue from mice found that 55% (11/20) of pigs were positive: 55% (11/20) and 45% (9/20) for brains and tongues, respectively. Mice were inoculated with material obtained from the samples and examined by various methods for resulting Toxoplasma infection (bioassay). Cyst detection in bioassay mice identified 25% (5/20) and immunohistochemistry 30% (6/20) of the samples pigs as positive for T. gondii. Histopathology of mice tissue could not detect parasite; only suggestive pathological changes such as inflammation with foci of necrosis were seen. The results indicated PCR of mice tissue as the most sensitive among those tested.

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“…BURG et al, 1989). Para a reação de PCR foram utilizados 10µM de primers externos T1 (5'-AGCGTCTCTCTTCAAGCAGC GTA-3') e T2 (5'-TCCGCAGCGACTTCTATCTCTGT-3') e para a reação de nested-PCR foram utilizados 10µM de primers internos T3 (5'-TGGGAATGAAAGAGACGCTAAT GTG-3') e T4 (5'-TTAAAGCGTTCGTGGTCAACTATCG-3'), segundo protocolo descrito por Yai et al (2003b).…”

Section: Pcr-rflp Multilocalunclassified

Sorodiagnóstico, isolamento e genotipagem de Toxoplasma gondii e investigação molecular de outros protozoários pertencentes à família Sarcocystidae em morcegos do estado de São Paulo

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Evaluation of experimental Toxoplasma gondii (Nicolle and Manceaux, 1909) infection in pigs by bioassay in mice and polymerase chain reaction

Cited by 32 publications

References 0 publications

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs

Comparison of methods for detection of Toxoplasma gondii in tissues of naturally exposed pigs

Sorodiagnóstico, isolamento e genotipagem de Toxoplasma gondii e investigação molecular de outros protozoários pertencentes à família Sarcocystidae em morcegos do estado de São Paulo

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