Evaluation of Eudragit-coated chitosan microparticles as an oral immune delivery system

Hori, Mika; Onishi, Hiraku; Machida, Yoshiharu

doi:10.1016/j.ijpharm.2005.04.008

Cited by 70 publications

(46 citation statements)

References 18 publications

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“…The pH dependent polymer Eudragit S100 is used in drug delivery for the production of colonic delivery systems [17]; it is an anionic copolymer based on methacrylic acid and methyl methacrylate which dissolves above pH 7. Eudragit coated microparticles were produced for the colonic release of therapeutics [16,18] or proteins [19], but it has not yet been applied for the coating of encapsulated cells. A variety of materials have been tested for the encapsulation of living cells; alginate [4,6,8,12,15,[20][21][22][23] is the most common and versatile, chitosan [9,23], gelatin [22,24], cellulose [23,25], agarose [23,26], dextran [1], carrageenan [9,12,27], poly(lactide-co-glycolide) (PLGA) [1,23], Poly (Ethylene Glycol) [23,28] all have been used individually, and in blends [1,27].…”

Section: Introductionmentioning

confidence: 99%

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Morelli

Holdich

Dragosavac

2017

Journal of Membrane Science

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Citation: MORELLI, S., HOLDICH, R. and DRAGOSAVAC, M., 2017. Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification. Journal of Membrane Science, 524, Additional Information:• This paper was accepted for publication in the journal Journal of This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. conditions. The liquid drops were loaded with increasing amount of yeast (3.14 x 10 7 to 3.14 x 10 8 cells/ mL). The stability and uniformity of the emulsions was independent of the cell concentration. PTFE coated hydrophobic membrane produced smaller W/O drops compared to FAS coated membranes. The liquid polymeric droplets were solidified in solid particles using thermal gelation and/or ionic crosslinking, obtaining yeast encapsulated particles sized ~100 µm.The pH sensitive polymer, Eudragit S100, was used as a coating to create gastro resistant particles suitable for intestinal-colonic targeted release. Viability of the released yeast cells was demonstrated using fluorescence probes and checking cell glucose metabolism with time. AbbreviationsW/O emulsion, water in oil

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Section: Introductionmentioning

confidence: 99%

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Morelli

Holdich

Dragosavac

2017

Journal of Membrane Science

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“…Vitamin B 12 (cobalamin) has its own intestinal uptake pathway by which it can be transported to the systemic circulation. By this mechanism, a molecule of intrinsic factor (IF), secreted by the parietal cells in the stomach, interacts and binds to one molecule of free vitamin B 12 .…”

Section: Introductionmentioning

confidence: 99%

“…By this mechanism, a molecule of intrinsic factor (IF), secreted by the parietal cells in the stomach, interacts and binds to one molecule of free vitamin B 12 . The resulting complex [vitamin B 12 -IF] moves down the gastrointestinal tract until it reaches the ileum. In this region, the complex is recognized by the intrinsic factor specific receptors (IF-CR) which are located on the surface of the ileal epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…Then, this complex is internalized by receptor-mediated endocytosis. Vitamin B 12 is subsequently transported to the systemic circulation and complexed with cobalamin-binding protein [Transcobalamin II (TCII)] (1, 2). This specific of vitamin B 12 uptake system has been explored in the last years to enhance the oral delivery of poorly available therapeutic molecules including, granulocyte colony stimulating factor, erythropoietin, and luteinizing hormone-releasing hormone (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, according to the limited uptake capacity of vitamin B 12 (2.4 µg/day for adult) (6), the amount of the therapeutic molecule that can be delivered as conjugate is limited. An attractive solution to overcome theses problems would be the incorporation of drug in nanoparticles coated with vitamin B 12 . This can offer both high loading capacity of the therapeutic molecules and the protection against the aggressive gut conditions (pH and enzymatic degradation).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Bioadhesive Capacity and Immunoadjuvant Properties of Vitamin B12-Gantrez Nanoparticles

et al. 2008

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pH‐responsive poly(DMAPMA‐co‐HEMA)‐based hydrogels for prolonged release of 5‐fluorouracil

Mishra

Ramasamy

Majeed

2012

J of Applied Polymer Sci

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This work reports the synthesis and characterization of pH-sensitive hydrogel (PSHs) system composed of N [-3(dimethylamino)propyl] methacrylamide (DMAPMA) and 2-hydroxyethyl methacrylate (HEMA). This hydrogel was prepared efficiently by solution copolymerization method using N,N-methylene bisacrylamide as crosslinker and sodium persulfate/ammonium persulfate as joint initiator system. The chemical structure of the hydrogel was confirmed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. Thermal stability and morphology of the hydrogels were assessed by thermogravimetric analysis and field emission scanning electron microscopy. 5-Fluorouracil (5-FU), an anticancer drug was loaded in the hydrogels to investigate their drug release properties using a human colon cancer cell line (DLD-1). The pH (pH 1.2-7.4) as well as temperature-sensitive swelling of the hydrogel was also determined. The morphological analysis of the resulting hydrogel revealed a highly interconnected macroporous interior with pore size ranging from 10 to 100 lm in size. The swelling of the hydrogel was highly influenced by pH of the surrounding medium and higher swelling ratio was observed at simulated intestinal fluid (SIF). The 5-FU release was found to be more efficient in SIF as compared with simulated gastric fluid. The results showed that the 5-FU released from the hydrogel remained biologically active and the developed hydrogel was not cytotoxic. The hydrogel with a 50 : 50 feed ratio of DMAPMA and HEMA was better than the other developed hydrogels. The PSH is furthermore safe for colon-targeted delivery of 5-FU. V C 2012Wiley Periodicals, Inc. J Appl Polym Sci 126: E98-E107, 2012

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Evaluation of Eudragit-coated chitosan microparticles as an oral immune delivery system

Cited by 70 publications

References 18 publications

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Evaluation of Bioadhesive Capacity and Immunoadjuvant Properties of Vitamin B12-Gantrez Nanoparticles

pH‐responsive poly(DMAPMA‐co‐HEMA)‐based hydrogels for prolonged release of 5‐fluorouracil

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