2001
DOI: 10.1081/css-100103000
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Evaluation of Effect of Washing Procedures on Mineral Analysis of Orange and Peach Leaves Sprayed With Seaweed Extracts Enriched With Iron

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Cited by 47 publications
(22 citation statements)
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“…The youngest developed leaves were sampled at 29, 39, and 49 d in green bean, 21 and 70 d in tomato rockwool and 13, 62, and 146 d in tomato soil mulch experiments after the treatment application. Sampled leaves were separated and washed with Tween 80 in 0.1 M HCl for 10 s (Álvarez‐Fernández et al, 2001), and then with abundant distilled water, weighed and dried. After dry mineralization, micronutrient concentrations were determined in leaves by AAS (PerkinElmer AAnalyst 800).…”
Section: Methodsmentioning
confidence: 99%
“…The youngest developed leaves were sampled at 29, 39, and 49 d in green bean, 21 and 70 d in tomato rockwool and 13, 62, and 146 d in tomato soil mulch experiments after the treatment application. Sampled leaves were separated and washed with Tween 80 in 0.1 M HCl for 10 s (Álvarez‐Fernández et al, 2001), and then with abundant distilled water, weighed and dried. After dry mineralization, micronutrient concentrations were determined in leaves by AAS (PerkinElmer AAnalyst 800).…”
Section: Methodsmentioning
confidence: 99%
“…Washing with acid detergent proved to be most efficient with regard to Fe, Mn, and Zn content. In another study, washing with distilled water and Twin-80 acidulated with 0.1M HCl proved to be most effective with regard to concentration of macroand micronutrients (Alvarez-Fernandez et al 2001). A good procedure for drying of the leaf samples involved blotting of samples after rinsing with deionized water, using ash-free filter paper sheets, placing leaves in brand-new paper bags, and then drying in an oven at 608 to 658C to a constant weight (Zekri and Obreza 2003a).…”
Section: A Leaf Analysismentioning
confidence: 99%
“…During the experiment, SPAD readings with a chlorophyll meter (Minolta SPAD-502) were taken for all the leaf stages (average of three readings per leaf) at several times, although only values measured for the second leaf stage (the youngest fully open leaf at the start of the treatment period) have been presented in the results, since they were the most representative of the whole plant.Whole plants were sampled 24 days (one pair of plants) and 37 days (two pairs of plants) after transplanting. The sampled roots and leaves were separated and washed first with Tween 80 in 0.1 M HCl for 10 s(14) and then with abundant distilled water, weighed, and dried. Micronutrients were determined in the leaves after the dry digestion procedure by AAS.…”
mentioning
confidence: 99%