Evaluation of DNA damage in Type 2 diabetes mellitus patients with and without peripheral neuropathy: A study in South Indian population

Abstract

Background:The increasing incidence of Type 2 diabetes mellitus globally has collaterally increased the incidence of diabetes-associated complications such as neuropathy. Oxidative stress induced DNA damage is one of the mechanisms implicated in the pathogenesis of diabetic complications. Here we aimed to evaluate the extent of DNA damage in diabetes patients with and without clinical neuropathy using the Cytokinesis Block Micronucleus Cytome assay, in a group of South Indian population.Materials and Methods:The Cytokinesis Block Micronucleus Cytome assay was performed in lymphocyte cultures of 42 type 2 diabetes patients (22 with neuropathy and 20 without neuropathy) and 42 age and sex matched controls. Nuclear aberrations like Nuclear Buds, Nucleoplasmic Bridges and Micronuclei were analyzed.Results:The frequency of nuclear aberrations in diabetes patients with neuropathy was higher than compared to diabetes patients without neuropathy. The mean frequencies of nuclear aberrations per cell in diabetes patients with neuropathy and without neuropathy were 0.02 ± 0.02 and 0.01 ± 0.01, respectively. This was significantly higher than in the controls (0.002 ± 0.002) (P < 0.0001). An increasing trend of nuclear aberrations in correlation with the duration of diabetes was observed.Conclusion:This study highlights the use of the Cytokinesis Block Micronucleus Cytome assay as a potent tool for the identification of DNA damage, which may prove to be useful biomarker to assess the severity diabetes-associated complications such as neuropathy. Implementation of this technique at the clinical level would potentially enhance the quality of management of patients with diabetes and its complications like neuropathy.

Influence of Body Mass Index and Duration of Disease on Chromosome Damage in Lymphocytes of Patients with Diabetes

“…Nevertheless, our study quite clearly indicated that the frequency of both CTAs and CSAs depends on the duration of the disease, not the type of diabetes. Interestingly, the same type of association has been reported in one CA [ 28 ] and several MN studies in T2DM patients [ 33 , 34 , 35 ]. Conversely, other studies failed to find a correlation between the frequency of MN and disease duration in T2DM [ 36 , 37 ].…”

“…Some authors revealed increased CAs or MN levels in patients with diabetic neuropathy [ 30 , 34 ] or nephropathy [ 33 ]. We did not find any association between the frequency of CAs and diabetes complications, but our sample size was small and, consequently, the statistical power of such analysis was quite low.…”

Susceptible and Prognostic Genetic Factors Associated with Diabetic Peripheral Neuropathy: A Comprehensive Literature Review

“…No genetic variants were evaluated in this study. The data demonstrated that the frequency of DNA damage was significantly higher in the T2D patients with DPN than in the controls [ 59 ]. Stoian et al conducted a study in the University Center of Tırgu Mures, Romania.…”