Evaluation of DNA Damage and Expressions of DNA Repair Gene in Occupationally Lead Exposed Workers (Jodhpur, India)

Singh, Preeti; Mitra, Prasenjit; Goyal, Taru; Sharma, Shailja; Sharma, Praveen

doi:10.1007/s12011-020-02298-2

Cited by 12 publications

(4 citation statements)

References 37 publications

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“…There has been an alarming increase in cancer, bronchial issues, and neurological disorders in Jodhpur City in the past few years [25][26][27][28] Site four where vegetables were most toxic is a slum area, where mostly lower-income group people reside, they buy these toxic cheap vegetables from local vendors near residential areas, small shops, and community markets where vegetables are brought from the areas irrigated with toxic effluent waters could well be due to severe and pressing issues of heavy metal toxicity exposure among the general population. Enforcing a strict ban on irrigation with effluent water should be the utmost priority.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of Heavy Metal Toxicity in the Vegetables Using ICP-MS and AAS

Kumari,

Kachhwaha,

Mishra

2024

Orient. J. Chem

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Our study was conducted between January and September 2023, and aimed to evaluate the presence of toxic heavy metals in vegetables cultivated using effluent and sewage water in Jodhpur city. Eight vegetable samples were obtained from four distinct locations, and the concentrations of seven harmful heavy metals (Cu, Zn, Pb, Cd, Cr, Ni, and Fe) were examined. The initial analysis employed ICP-MS, followed by determining the concentration of heavy metals in the vegetables through atomic absorption spectrometry (AAS). The Heavy Metal Pollution Index (HPI) was computed to assess pollution levels at each site. Comparative analysis of vegetable samples using both ICP-MS and AAS indicated elevated levels of lead, cadmium, and chromium pollution in vegetables from all four sites. Calculations further confirmed the contamination of the sites with toxic heavy metals. The order of metal concentration based on allowable limits was Fe>Cd>Cr>Pb>Ni>Zn>Cu. The overall order of observed heavy metal concentrations site-wise was site 4 > site 3 > site 2 > site 1. Many samples were obtained from local vendors near residential areas and community markets frequented by the public. This highlights a crucial issue of heavy metal toxicity exposure to the general population, emphasizing urgent government action, including essential restrictions and measures, to address this pressing problem.

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Section: Discussionmentioning

confidence: 99%

A Comparative Study of Heavy Metal Toxicity in the Vegetables Using ICP-MS and AAS

Kumari,

Kachhwaha,

Mishra

2024

Orient. J. Chem

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“…For example, prolonged duration times of exposure should be considered to cover sub-chronic and chronic effects in the in vitro models [ 34 ]. According to all of the epidemiological studies reviewed in this article, there was significant down-regulation of the DNA repair gene expression and high blood Pb levels in exposed groups as compared to control groups, except for in the work of Bakheet et al, who reported no significant changes in the expression of DNA repair genes in the exposed volunteers, which might be due to the normal blood Pb levels of the exposed group [ 36 ]. Therefore, it can be suggested that Pb accumulation levels may be associated with the expression level of DNA repair genes.…”

Section: Discussionmentioning

confidence: 99%

“…A previous cohort study showed a significant decrease of OGG1-2a in Pb-exposure in workers from a construction site when compared with age-matched controls [ 35 ]. Moreover, Singh et al (2020) reported significantly higher blood lead levels (BLL) and significant down-regulation of OGG1, XRCC1, and XPD in Pb-exposed workers as compared with the unexposed group [ 36 ]. However, a negative result for the effect of Pb on the DNA repair gene expression was documented.…”

Section: Study On the Effect Of Lead On Dna Repair-related Genesmentioning

confidence: 99%

Genotoxic Effects of Lead and Their Impact on the Expression of DNA Repair Genes

Hemmaphan

Bordeerat

2022

IJERPH

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Exposure to lead (Pb) continues to be a significant worldwide problem. Pb is a highly poisonous heavy metal affecting several organ systems in the body. Although Pb has been shown to be genotoxic to experimental animals and humans, the underlying mechanisms are still not understood. An indirect mechanism related to the inhibition of DNA repair systems by Pb has been suggested. Heavy metals can interfere with the activities of several proteins and gene expressions. Recent studies gathered in this review article demonstrated an altered expression of DNA repair genes due to Pb toxicity. However, their findings are conflicting. Furthermore, the interaction of Pb and epigenetic mechanisms regulating gene expression may have a crucial role in the inhibition of DNA repair systems. Therefore, additional studies are needed to evaluate these findings and to obtain a complete picture of the genotoxic properties of Pb and the underlying mechanisms that may have a crucial role in carcinogenesis.

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“…Human exposure to lead (Pb) and its compounds occurs mainly in occupational settings, especially in industrial processes such as smelting, pottery, shipbuilding, Pb-based painting, Pb-containing pipes, car battery recycling, grids, firearms industry, pigments, printing, among others [ 1 ]. Some other sources, such as canned food, cosmetics, lead pipes used in water supply, and the use of certain herbal products, can also contribute to Pb exposure in humans [ 2 ]. McFarland et al [ 3 ] showed that humans may also have underpredicted high-lead exposures owing to leaded paints and pipes, which tend to aggregate within communities with high rates of homes with lead service lines and lead paint in disrepair.…”

Section: Introductionmentioning

confidence: 99%

Association between Polymorphisms of Hemochromatosis (HFE), Blood Lead (Pb) Levels, and DNA Oxidative Damage in Battery Workers

Gomes

Devóz

Rocha

et al. 2023

IJERPH

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Occupational exposure to lead (Pb) continues to be a serious public health concern and may pose an elevated risk of genetic oxidative damage. In Brazil, car battery manufacturing and recycling factories represent a great source of Pb contamination, and there are no guidelines on how to properly protect workers from exposure or to dispose the process wastes. Previous studies have shown that Pb body burden is associated with genetic polymorphisms, which consequently may influence the toxicity of the metal. The aim of this study was to assess the impact of Pb exposure on DNA oxidative damage, as well as the modulation of hemochromatosis (HFE) polymorphisms on Pb body burden, and the toxicity of Pb, through the analysis of 8-hydroxy-2′-deoxyguanosine (8-OHdG), in subjects occupationally exposed to the metal. Male Pb-exposed workers (n = 236) from car battery manufacturing and recycling factories in Brazil participated in the study. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS and urinary 8-OHdG levels were measured by LC-MS/MS, and genotyping of HFE SNPs (rs1799945, C → G; and 1800562, G → A) was performed by TaqMan assays. Our data showed that carriers of at least one variant allele for HFE rs1799945 (CG + GG) tended to have higher PLL than those with the non-variant genotype (β = 0.34; p = 0.043); further, PLL was significantly correlated with the levels of urinary 8-OHdG (β = 0.19; p = 0.0060), while workers that carry the variant genotype for HFE rs1800562 (A-allele) showed a prominent increase in 8-OHdG, as a function of PLL (β = 0.78; p = 0.046). Taken together, our data suggest that HFE polymorphisms may modulate the Pb body burden and, consequently, the oxidative DNA damage induced by the metal.

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Evaluation of DNA Damage and Expressions of DNA Repair Gene in Occupationally Lead Exposed Workers (Jodhpur, India)

Cited by 12 publications

References 37 publications

A Comparative Study of Heavy Metal Toxicity in the Vegetables Using ICP-MS and AAS

A Comparative Study of Heavy Metal Toxicity in the Vegetables Using ICP-MS and AAS

Genotoxic Effects of Lead and Their Impact on the Expression of DNA Repair Genes

Association between Polymorphisms of Hemochromatosis (HFE), Blood Lead (Pb) Levels, and DNA Oxidative Damage in Battery Workers

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