Evaluation of Cilia Function in Rat Trachea Reconstructed Using Collagen Sponge Scaffold Seeded with Adipose Tissue‐Derived Stem Cells

Abstract

The tracheal lumen is essential for conducting air to the lung alveoli and for voice production. However, patients with severe tracheal stenosis and malignant tumors invading the trachea often require tracheal resection. Recently, various reported tissue engineering methods for tracheal reconstruction show that regeneration of ciliated epithelium in the reconstructed areas, as well as preservation of the luminal structure is possible. However, only few studies report on the mucociliary transport function in reconstructed tracheae. We investigated mucociliary transport function within rat tracheal epithelium, reorganized after autologous adipose tissue‐derived stem cell (ASC) transplantation. Rat ASCs were expanded in culture, and then seeded in a collagen sponge, which was physically supported with a polypropylene framework. The ASC‐seeded collagen sponge was transplanted into the rat tracheal defect. We then examined the motility and transport function of cilia generated in the transplanted area using ciliary beat frequency (CBF) and microsphere movement analyses. Our data suggested that autologous ASC transplantation promoted ciliogenesis, consistent with previous reports. The CBF analysis revealed that motility of the cilia generated in the ASC group was comparable to that observed in the normal rat tracheal epithelium. Transport function in the ASC group was higher than that in the control group. These data suggested that autologous ASC transplantation increased ciliated cells in the reconstructed area without significantly disrupting cilia motility, thereby promoting transport function regeneration. Autologous ASC transplantation is expected to be beneficial in morphological and functional regeneration of tracheal epithelium. Anat Rec, 303:471–477, 2020. © 2019 American Association for Anatomy

3D Printed Biomimetic PCL Scaffold as Framework Interspersed With Collagen for Long Segment Tracheal Replacement

“…Good biocompatibility and suitable biodegradability are prerequisite for a scaffold to be used in tracheal tissue engineering. Previous extensive studies have demonstrated that both PCL and collagen are biocompatible (Fu et al, 2015;Jia et al, 2020;Nakamura et al, 2020;Semitela et al, 2020), so we did not specifically test the biocompatibility of the composite using a cell proliferation assay (e.g., a CCK-8 kit) or cell viability assay (e.g., live-dead staining). Nevertheless, our results indicated that the biomimetic scaffold comprised of PCL and collagen support tracheal cartilage formation both in vitro and in vivo, which indirectly indicates that the 3D-printed PCL scaffold with embedded collagen exhibited satisfactory biocompatibility.…”

Cartilaginous Extracellular Matrix Enriched with Human Gingival Mesenchymal Stem Cells Derived “Matrix Bound Extracellular Vesicles” Enabled Functional Reconstruction of Tracheal Defect

“…With the aid of polymeric or metal mesh, collagen foam infused with fibrin gel, or non‐woven mesh mediated cell produced ECM have demonstrated promising results in vivo. [ 39,49,50 ] Departing from above designs, we fabricated cACM‐PGS construct. Owing to the similar elasticity and adequate stiffness, the firm anchorage between cACM and PGS pores was well maintained, which was critical to physical integrity and biological efficacy.…”

Tissue engineering applications in otolaryngology—The state of translation

“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate laryngeal tissue engineering 85‐87,91,92,94,96,97,198‐306 …”