Evaluation of Cardioprotective Activity of Fulvic Acid Against Isoproterenol Induced Oxidative Damage in Rat Myocardium

Abstract

The aim of study was to evaluate cardio protective activity of fulvic acid in Isoprenaline induced cardiac toxicity. In current investigation, female/male wistar rats were divided into five groups, normal, control, Fulvic acid 100mg/kg, Fulvic acid 200mg/kg, and Fulvic acid 300 mg/kg. Isoprenaline 85 mg/kg was administered on 29 th and 30 th day during study period to all except normal group. Fulvic acid was administered to respective groups once daily for total 30 days, on the last day of study, the animals were anesthetized to record ECG and BP (by cannulating carotid artery), blood was collected from carotid artery and SGOT, LDH And CK-MB were estimated, Animals were sacrificed to isolate heart and preparation of tissue homogenate. The antioxidant status is analyzed by measuring MDA content, SOD CAT, GSH activity. The tissue sample is also preserved for histological studies. Isoprenaline causes cardiac damage which was manifested by alteration in serum cardiac markers, antioxidant markers, ECG and hemodynamic and histological changes. These alterations were restored due to treatment with fulvic acid 300mg/kg. With data obtained in study it have been concluded that fulvic acid treatment for 4 weeks protect the heart of rat from cardiotoxicity as a result of isoprenaline administration.

Eco-Corona vs Protein Corona: Effects of Humic Substances on Corona Formation and Nanoplastic Particle Toxicity in Daphnia magna

“…However, it is noteworthy that, although there was an observed increase in the percentage of surviving animals in the NPP 100 FA group (Figure C), all genes were significantly induced (Figure ). This is in accordance with the observations that FA reduces oxidative damage by increasing the levels of antioxidant enzymes such as SOD, CAT, and GPx . In another aspect, with a higher negative charge than NOM and HA, FA has a better chance of interacting with the positive charge of the animal’s protein when it is in contact with the animal.…”

Cardioprotective potency of anthocyanin-rich extract of red cabbage against isoproterenol-induced myocardial infarction in experimental animals

“…4a). The current findings are in agreement with the results observed by Shikalgar and Naikwade (2018) and Derbali et al (2015). Elevation of relative heart weight of MI rats may be attributed to increment of water content and necrosis of myocardial muscle fibers followed by damage of tissue by inflammation of the cells (Rajadurai and Mainzen, 2007;Shikalgar and Naikwade, 2018).…”

“…This model is commonly used for the evaluation of the efficacy of new drugs or alternative treatments. ISO produces unrepairable damage in the cells and finally leads to necrosis of the myocardium (Shikalgar and Naikwade, 2018). ISO injection led to hypoxia ischemia through direct action on cardiac cardioprotective and vascular β-receptors, which finally induce necrosis of myocardium (Dianita et al, 2015).…”

Therapeutic Potential of Fulvic Acid in Chronic Inflammatory Diseases and Diabetes

“…In addition, the most promising in vivo study regarding the antioxidant ability of FvA is the reduction of oxidative stress markers after isoproterenol (ISO) induced myocardial damage in rats. Shikalgar and Naikwade show that FvA at 300 mg/kg/day for 4 weeks decreases lipid peroxidation and myocardial damage markers after ISO and significantly increases GSH, SOD, and catalase (CAT) levels [ 33 ]. Another study confirms this information in fish.…”

“…FvA might fit as an adjunct treatment to reduce markers of oxidative stress and inflammation as FvA can act in a similar manner to NSAIDs [ 20 ]. FvA might also reduce oxidative damage and increase antioxidant enzymes like SOD, CAT, and GPx [ 33 ]. Beta cells, which are responsible for insulin production, undergo oxidative damage during T2DM [ 51 ].…”