Evaluation of bioefficacy of three Citrus essential oils against the dengue vector Aedes albopictus (Diptera: Culicidae) in correlation to their components enantiomeric distribution

Abstract

Laboratory experiments were conducted to study the bioefficacy against Ae. albopictus of three Citrus essential oils, derived from peels of Citrus sinensis, Citrus limon, and Citrus paradise and of their components. Chiral gas chromatography analysis revealed the dominant occurrence of R-(+)-limonene and (-)-β-pinene in all three essential oils while in the case of lemon oil γ-terpinene, neral, and geranial detected also among other components. The tested Citrus essential oils were toxic against mosquito larvae with LC(50) values ranging from 25.03 to 37.03 mg l(-1). Among citrus essential oils components tested, γ-terpinene was the most toxic (LC(50) = 20.21 mg l(-1)) followed by both enantiomeric forms of limonene (LC(50) = 35.99 and 34.89 mg l(-1), for R-(+)-limonene and S-(-)-limonene, respectively). The delayed toxic effects after exposure of larvae to sublethal (LC(50)) doses were also investigated for citrus essential oils and their major component R-(+)-limonene, indicating a significant reduction of pupal survival. In repellent bioassays, lemon essential oil, S-(-)-limonene, citral (mixture of neral\geranial) and (+)-β-pinene were the most effective compared with other citrus essential oils and components against adult mosquitoes. Repellent bioassays also revealed that limonenes and β-pinenes showed an isomer dependence repellent activity. Finally, according to enantiomeric distribution of limonene and α- and β-pinene, the repellency of lemon essential oil is possibly attributed to the presence of citral.

Assessment of the repellent effect of Lippia alba essential oil and major monoterpenes on the cattle tick Rhipicephalus microplus

“…Limonene and linalool have been demonstrated to possess repellent activity against insects, and fleas and ticks in dogs and cats (Environmental Protection Agency, 1994;Hebeish et al, 2008;Müller et al, 2009;Giatropoulos et al, 2012). Carvone has been reported to act as a repellent against A. americanum nymphs (Weldon et al, 2011).…”

Dengue Virus

“…Alternatives to temephos are methoprene, a synthetic compound that interferes with insect metamorphosis, and bti, a mosquito-specific, gut-toxic protein suspension derived from the bacterium Bacillus thuringiensis serotype H-14. Numerous larvicidal plant extracts have been identified in the laboratory (Giatropoulos et al, 2012;Kovendan et al, 2012Kovendan et al, , 2013Mahesh Kumar et al, 2013).…”

CHEMICAL COMPOSITION AND TOXICITY OF CITRUS ESSENTIAL OILS ON Dysmicoccus brevipes (HEMIPTERA: PSEUDOCOCCIDAE)

“…Their major component is the D-limonene, whose content vary from one species to another (BIZZO; HOVELL; REZENDE, 2009). Some researchers tested these oils as potential insecticides to control Spodoptera litura (Lepidoptera: Noctuidae), Myzuspersicae (Hemiptera: Aphididae) (ISMAN, 2000), Aedes albopictus (Diptera: Culicidae) and Musca domestica (Diptera: Muscidae) (GIATROPOULOS et al, 2012;KUMAR et al, 2012).…”