Evaluation of aptamers as molecular recognition elements for pathogens using capillary electrophoretic analysis

Aptamers-based assays for diagnostics, environmental and food analysis

“…Another aptamer for pathogens has been developed for Campylobacter jejuni by using electrophoretic analysis (McMasters and Stratis-Cullum, 2006). Campylobacter jejuni is the most commonly reported bacterial cause of foodborne infection in the United States and mishandling of raw poultry and consumption of undercooked poultry are the major risk factors for human campylobacteriosis (Altekruse et al, 1999).…”

Recent advances in recognition elements of food and environmental biosensors: A review

“…Pan et al (2005) used fluorescence in combination with real-time PCR to build up an optical sensor to give a quantitative as well as a qualitative assay for the detection of Salmonella enterica (type IVB pilus). Fluorescently labeled aptamers have also been used to detect whole cell Campylobacter jejuni using capillary electrophoresis with laser-induced fluorescence in which high-affinity detection was observed (McMasters and Stratis-Cullum, 2006). Collett et al (2005) developed an aptamer microarray to screen the affinity and specificity of a pool of anti-lysozyme RNA aptamers.…”

Aptamers for safety and quality assurance in the food industry: detection of pathogens

“…, 2006; Joshi et al. , 2009) but it has been reported that aptamers can also be selected to bind whole bacterial cells (McMasters & Stratis‐Cullum, 2006; Chen et al. , 2007; Hamula et al.…”

“…In response to these pressures, there has been a progressive shift towards high volume, centralised food production, combined with rapid distribution of ready meals to retailers. This has served to decrease the time between initial production and consumption and has therefore increased the need for rapid diagnostics to protect consumers from potential exposure to food pathogens Aptamers have been shown to have a great potential for use in future food safety in detection of bacteria (McMasters & Stratis‐Cullum, 2006; Hamula et al. , 2008; Joshi et al.…”