Evaluation of antitumor activity and in vivo antioxidant status of Anthocephalus cadamba on Ehrlich ascites carcinoma treated mice

Dolai, Narayan; Karmakar, Indrajit; Kumar, Rajesh; Kar, Biswakanth; Bala, Asis; Haldar, Pallab Kanti

doi:10.1016/j.jep.2012.05.050

Cited by 60 publications

(52 citation statements)

References 15 publications

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“…The Ehrlich tumor was initially described as a spontaneous murine mammary adenocarcinoma (Dolai et al, 2012a). It is a rapidly growing carcinoma with very aggressive behavior and is able to grow in almost all strains of mice.…”

Section: Introductionmentioning

confidence: 99%

Antitumor activity and antioxidant property ofCurcuma caesiaagainst Ehrlich’s ascites carcinoma bearing mice

Karmakar

Dolai

Kumar

et al. 2013

Pharmaceutical Biology

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Section: Introductionmentioning

confidence: 99%

Antitumor activity and antioxidant property ofCurcuma caesiaagainst Ehrlich’s ascites carcinoma bearing mice

Karmakar

Dolai

Kumar

et al. 2013

Pharmaceutical Biology

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“…Five to seven-day-old Ehrlich tumor cells, 0.5 mL at 2.0 × 10 6 cells/mL, were implanted in the peritoneal cavity of the female mice ( n =6/group) [26]. One day after inoculation, EOM (50, 100 or 150 mg/kg) and its major component (30 or 60 mg/kg) were dissolved in 5% ( v /v) Tween-80 in 0.9% ( w / v ) NaCl, and administered for nine consecutive days (i.p.).…”

Section: Methodsmentioning

confidence: 99%

“…It was determined the total viable cancer cells by trypan blue assay [26]. Tumor weights were measured by taking the mice’s weights before and after the collection of the ascitic fluid from peritoneal cavity, and expressed in grams (g).…”

Section: Methodsmentioning

confidence: 99%

Toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component

Rolim

Meireles

Batista

et al. 2017

BMC Complement Altern Med

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BackgroundThe essential oil from Mesosphaerum sidifolium (L’Hérit.) Harley & J.F.B.Pastore (syn. Hyptis umbrosa), Lamiaceae (EOM), and its major component, have been tested for toxicity and antitumor activity.MethodsEOM was obtained from aerial parts of M. sidifolium subjected to hydro distillation, and gas chromatography-mass spectrometry was used to characterize the EOM chemical composition. The toxicity was evaluated using haemolysis assay, and acute toxicity and micronucleus tests. Ehrlich ascites carcinoma model was used to evaluate the in vivo antitumor activity and toxicity of EOM (50, 100 and 150 mg/kg), and fenchone (30 and 60 mg/kg) after 9 d of treatment.ResultsThe EOM major components were fenchone (24.8%), cubebol (6.9%), limonene (5.4%), spathulenol (4.5%), β-caryophyllene (4.6%) and α-cadinol (4.7%). The HC50 (concentration producing 50% haemolysis) was 494.9 μg/mL for EOM and higher than 3000 μg/mL for fenchone. The LD50 for EOM was approximately 500 mg/kg in mice. The essential oil induced increase of micronucleated erythrocytes only at 300 mg/kg, suggesting moderate genotoxicity. EOM (100 or 150 mg/kg) and fenchone (60 mg/kg) reduced all analyzed parameters (tumor volume and mass, and total viable cancer cells). Survival also increased for the treated animals with EOM and fenchone. For EOM 150 mg/kg and 5-FU treatment, most cells were arrested in the G0/G1 phase, whereas for fenchone, cells arrested in the S phase, which represents a blockage in cell cycle progression. Regarding the toxicological evaluation, EOM induced weight loss, but did not induce hematological, biochemical or histological (liver and kidneys) toxicity. Fenchone induced decrease of AST and ALT, suggesting liver damage.ConclusionsThe data showed EOM caused in vivo cell growth inhibition on Ehrlich ascites carcinoma model by inducing cell cycle arrest, without major changes in the toxicity parameters evaluated. In addition, this activity was associated with the presence of fenchone, its major component.

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“…Antitumor activity and in vivo antioxidant status of A. cadamba on Ehrlich ascites carcinoma treated mice was already proved by our research groups (Dolai et al 2012a). This plant possesses good in vivo antioxidant property on liver and kidney tissues against Ehrlich ascites carcinoma cells.…”

Section: Introductionmentioning

confidence: 75%

“…Miq. (Rubiaceae) is commonly known as BKadamba^in Sanskrit and Hindi, which is frequently found in moist deciduous evergreen forests and widely distributed throughout the greater part of India (Dolai et al 2012a). Various plant parts are used as folk medicine in the treatment of fever, uterine complaints, hematological diseases, skin diseases, leprosy, dysentery, tumor and it also improve the quality of semen (Umachigi et al 2007;Alam et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of Anthocephalus cadamba stem bark fractions intercede anti-inflammatory and carbon tetrachloride induced hepatotoxicity in rats

Dolai

Kumari

Islam³

et al. 2015

Orient Pharm Exp Med

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Anthocephalus cadamba (Roxb.) Miq. (Rubiaceae) is commonly known as BKadamba^, frequently found in moist deciduous evergreen forests. The present study was performed to evaluate the anti-inflammatory and hepatoprotective potential of A. cadamba fractions in relation to the free radical scavenging properties to corroborate and establish scientific evidence for its ethno-botanical uses. Quantification assay were determined for total phenolic and flavonoid compounds. Free radical scavenging properties were assessed by 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide, peroxynitrite, superoxide, hypochlorous acid scavenging methods. Protein denaturation and human red blood cell membrane stabilization methods were performed to determine anti-inflammatory activity. Furthermore, Hepatocuretive potential was evaluated using carbon tetrachloride intoxicated (CCl 4 ) rat's model. Methanol fraction of A. cadamba (MEAC) was found to be the most potent free radical scavenger in all in vitro models. The higher inhibition properties of MEAC positively correlated with total phenolic (164.75±3.83 mg GAE/g) and flavonoid compounds (51.20± 2.15 mg QE/g). The MEAC showed significant (p<0.01) antiinflammatory activity in all in-vitro methods. The A. cadamba significantly (p<0.01) decreased levels of serum glutamic oxaloacetate transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase and total bilirubin in the treated groups when compare to CCl 4 control group. Treatment with A. cadamba significantly (p<0.01) increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl 4 control group. These results indicated that A. cadamba possess anti-inflammatory and hepatoprotective property which may be mediated through augmentation of antioxidant defenses.

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Evaluation of antitumor activity and in vivo antioxidant status of Anthocephalus cadamba on Ehrlich ascites carcinoma treated mice

Cited by 60 publications

References 15 publications

Antitumor activity and antioxidant property ofCurcuma caesiaagainst Ehrlich’s ascites carcinoma bearing mice

Antitumor activity and antioxidant property ofCurcuma caesiaagainst Ehrlich’s ascites carcinoma bearing mice

Toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component

Inhibitory effects of Anthocephalus cadamba stem bark fractions intercede anti-inflammatory and carbon tetrachloride induced hepatotoxicity in rats

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