Evaluation of antioxidant and anti-inflammatory potency of Lepidium pinnatifidum Ledeb

Bibi, Saira; Anwar, Munazza; Hashmi, Huma Farooque; Khan, Muhammad Rashid

doi:10.1186/s40816-020-00170-3

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…The antioxidant activity of phenolics and flavonoids primarily depends on the presence of hydroxyl groups [26]. In a previous study on A. bracteosa, the highest amount of phenolic content was found in crude extracts prepared in more polar solvents (10.75 ± 0.70 µg GAE/mg DW of methanolic extracts) than the non-polar solvent systems (0.33 ± 0.0 µg GAE/mg DW demonstrated by n-hexane extract).…”

Section: Discussionmentioning

confidence: 88%

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

et al. 2021

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Ajuga bracteosa Wall. ex Benth. is an endangered medicinal herb traditionally used against different ailments. The present study aimed to create new insight into the fundamental mechanisms of genetic transformation and the biological activities of this plant. We transformed the A. bracteosa plant with rol genes of Agrobacterium rhizogenes and raised the regenerants from the hairy roots. These transgenic regenerants were screened for in vitro antioxidant activities, a range of in vivo assays, elemental analysis, polyphenol content, and different phytochemicals found through HPLC. Among 18 polyphenolic standards, kaempferol was most abundant in all transgenic lines. Furthermore, transgenic line 3 (ABRL3) showed maximum phenolics and flavonoids content among all tested plant extracts. ABRL3 also demonstrated the highest total antioxidant capacity (8.16 ± 1 μg AAE/mg), total reducing power, (6.60 ± 1.17 μg AAE/mg), DPPH activity (IC50 = 59.5 ± 0.8 μg/mL), hydroxyl ion scavenging (IC50 = 122.5 ± 0.90 μg/mL), and iron-chelating power (IC50 = 154.8 ± 2 μg/mL). Moreover, transformed plant extracts produced significant analgesic, anti-inflammatory, anticoagulant, and antidepressant activities in BALB/c mice models. In conclusion, transgenic regenerants of A. bracteosa pose better antioxidant and pharmacological properties under the effect of rol genes as compared to wild-type plants.

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Section: Discussionmentioning

confidence: 88%

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

et al. 2021

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“…The antioxidant activity of phenolics and avonoids primarily depends on the presence of hydroxyl groups [33]. Current study demonstrated signi cantly higher values of TPC and TFC in transformed regenerants as compared to untransformed plants.…”

Section: Discussionmentioning

confidence: 48%

Ajuga Bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Rubnawaz¹,

Kayani²,

Akhtar³

et al. 2021

Preprint

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Ajuga bracteosa Wall. ex Benth is an endangered medicinal herb used against different ailments in folklore medicines. Here, we aimed to create a new insight to the fundamental mechanisms of genetic transformation in the ethnomedicinal usage of this plant. We transformed the plant with rol genes of Agrobacterium rhizogenes and raised the regenerants from the hairy roots. The transgenic regenerants were screened for in vitro antioxidant activities, a range of in vivo assays, and linked the activities with elemental analysis, polyphenol content and different phytochemicals found through HPLC. Among 18 polyphenolic standards, kaempferol was found most abundant in all transgenic lines (up to 101.26 ± 6 µg/mg). Furthermore, among all tested plant extracts, transgenic line 3 (ABRL3) showed maximum phenolics (13.39 ± 2µg GAE/mg) and flavonoids content (4.75 ± 0.16 µg QE/mg). ABRL3 also demonstrated potent total antioxidant capacity (8.16 ± 1 µg AAE/mg), total reducing power, (6.60 ± 1.17 µg AAE/mg), DPPH activity (IC50 = 59.5 ± 0.8µg/mL), hydroxyl ion scavenging (IC50 = 122.5 ± 0.90 µg/mL), and iron chelating power (IC50 = 154.8 ± 2 µg/mL) among all plants. Transformed plant extracts also produced significant analgesic, anti-inflammatory, anticoagulant, and antidepressant properties in in vivo mice model as compared to control untransformed plant material. Additionally, no abnormal behavior or lethality was observed in any animal tested. In conclusion, transgenic regenerants of A. bracteosa pose better pharmacological properties under the effect of rol genes as compared to wild type plants.

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Evaluation of antioxidant and anti-inflammatory potency of Lepidium pinnatifidum Ledeb

Cited by 2 publications

References 31 publications

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential

Ajuga Bracteosa Transgenic Regenerants Display Better Pharmacological Potential

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