2015
DOI: 10.1111/rda.12612
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Abstract: This study evaluated the performance of an immunochromatographic test (ICT) for the diagnosis of canine brucellosis caused by Brucella canis, comparing its results with that of the rapid slide agglutination test with and without the use of 2-mercaptoethanol and the agar gel immunodiffusion test (AGID). The microbiological culture, PCR and clinical examination were used as reference. According to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and PCR in blood, sem… Show more

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Cited by 20 publications
(39 citation statements)
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“…The serological tests available for the diagnosis of brucellosis in dogs are characterized by low values of diagnostic sensitivity, allowing the accurate detection of infected dogs predominately during the bacteremic period; therefore, blood culture is considered the definitive method for canine brucellosis diagnosis (Keid et al., , ; Wanke et al., ; Zoha & Carmichael, ). The detection of infected dogs after the bacteremic period is a great challenge as several laboratory tests and multiple biological samples from each animal may be necessary to confirm infection (Keid, Soares, Vasconcellos, Chiebao, Megid, et al., ; Keid, Soares, Vasconcellos, Chiebao, Salgado, et al., ; Zoha & Carmichael, ).…”
Section: Resultsmentioning
confidence: 99%
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“…The serological tests available for the diagnosis of brucellosis in dogs are characterized by low values of diagnostic sensitivity, allowing the accurate detection of infected dogs predominately during the bacteremic period; therefore, blood culture is considered the definitive method for canine brucellosis diagnosis (Keid et al., , ; Wanke et al., ; Zoha & Carmichael, ). The detection of infected dogs after the bacteremic period is a great challenge as several laboratory tests and multiple biological samples from each animal may be necessary to confirm infection (Keid, Soares, Vasconcellos, Chiebao, Megid, et al., ; Keid, Soares, Vasconcellos, Chiebao, Salgado, et al., ; Zoha & Carmichael, ).…”
Section: Resultsmentioning
confidence: 99%
“…In this study, the diagnosis of brucellosis was based solely on the use of blood culture as positive results in this test are indicative of active infections and because of its high sensitivity when compared to the other laboratory tests available. Blood culture was proved to have a higher sensitivity when compared to the culture of other biological samples such as semen (Keid, Soares, Vasconcellos, Chiebao, Megid, et al., ), urine (Serikawa & Muraguchi, ) and vaginal discharge (Keid, Soares, Vasconcellos, Chiebao, Salgado, et al., ) and also when compared to the available serological tests (Keid et al., ; Wanke et al., ). PCR using blood showed a sensitivity similar or slight higher when compared to blood culture (Keid, Soares, Vieira, et al., ) and could be an alternative to the diagnosis.…”
Section: Resultsmentioning
confidence: 99%
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“…Há vários métodos de diagnóstico disponíveis para a brucelose nos cães, baseados tanto em testes sorológicos quanto em métodos microbiológicos e moleculares, com desempenho variável em sensibilidade, especificidade, rapidez e praticidade na identificação de cães acometidos (KEID et al, 2007a,b,c;KEID et al, 2009;WANKE et al, 2012;KEID et al, 2015;LIMA, 2018).…”
Section: Segundo Dados Disponibilizados Pela Fédération Cynologique Iunclassified