Evaluation of an established pericardium patch for delivery of mesenchymal stem cells to cardiac tissue

Vashi, Aditya V.; White, Jacinta F.; McLean, Keith M.; Neethling, William M.L.; Rhodes, David; Ramshaw, John A. M.; Werkmeister, Jerome A.

doi:10.1002/jbma.35335

Cited by 6 publications

(8 citation statements)

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“…This study contributes to recognize the effect of TX and STX decellularization method on macrophage activation for the purpose of designing biological implants composed of matrix remaining the pericardium decellularization. For instance, this matrix serves as structural support for the cell attachment and the reservoir of signaling factors,, or as raw material in the manufacture of cross‐linked biological implants . This research deserves further studies to elucidate the impact of both TX and STX methods of pericardium decellularization on the in vivo biomaterial performance at medium and long terms to understand the effect of pericardial bioscaffold composition on the inflammation resolution and remodeling process.…”

Section: Discussionmentioning

confidence: 99%

“…Pericardial biomaterials are extensively used in the manufacture of heart valves and abdominal meshes, usually as a fixed or cross‐linked matrix. Recently, the use of pericardium‐derived bioscaffolds has been spread to biomedical patches with characteristics suitable for delivery of different cells that act as templates for cell propagation and new cardiac tissue formation . These bioscaffolds are also used for inducing collagen deposition, and active tissue remodeling by invading host cells and good tissue integration in vivo .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the use of pericardium-derived bioscaffolds has been spread to biomedical patches with characteristics suitable for delivery of different cells that act as templates for cell propagation and new cardiac tissue formation. 3 These bioscaffolds are also used for inducing collagen deposition, and active tissue remodeling by invading host cells and good tissue integration in vivo. 4 It is of particular interest that the decellularization of pericardial tissue with nonionic detergents, for example, Triton X-100 (TX), preserves the collagen structural network, anisotropy, tensile modulus, tensile strength, and failure strain.…”

Section: Introductionmentioning

confidence: 99%

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The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

Mendoza-Novelo

Castellano

Padilla-Miranda

et al. 2016

J Biomedical Materials Res

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The extracellular matrix molecules remaining in bioscaffolds derived from decellularized xenogeneic tissues appear to be important for inducing cell functions conducting tissue regeneration. Here, we studied whether decellularization methods, that is, detergent Triton X-100 (TX) alone and TX combined with reversible alkaline swelling (STX), applied to bovine pericardial tissue, could affect the bioscaffold components. The in vitro macrophage response, subdermal biodegradation, and cell infiltration were also studied. The results indicate a lower leaching of fibronectin, but a higher leaching of laminin and sulfated glycosaminoglycans from tissues decellularized with STX and TX, respectively. The in vitro secretion of interleukin-6 and monocyte chemoattractant protein by RAW264.7 macrophages is promoted by decellularized bioscaffold leachates. A lower polymorphonuclear cell density is observed around decellularized bioscaffolds at 1-day implantation; concurrently showing a higher cell infiltration in STX- than in TX-implant. Cells infiltrated into TX-implant show a fibroblastic morphology at 7-day implantation, concurrently the capillary formation is observed at 14-day. Pericardial bioscaffolds suffer biodegradation more pronounced in STX- than in TX-implant. Both TX and STX decellularization methods favor a high leaching of basal lamina components, which presumably promotes a faster macrophage stimulation compared to nondecellularized tissue, and appear to be associated with an increased host cell infiltration in a rat subdermal implantation. Meanwhile, the connective tissue components leaching from TX decellularized bioscaffolds, unlike the STX ones, appear to be associated with an enhanced angiogenesis accompanied by an early-promoted fibroblastic cell transition. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2810-2822, 2016.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

Mendoza-Novelo

Castellano

Padilla-Miranda

et al. 2016

J Biomedical Materials Res

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“…The process provides a material that can have controlled properties, including high porosity along with high flexibility and controlled turnover . Another key aspect is that it still provides a cell friendly environment . Thus, a Cardiocel™ product has been examined as a natural extracellular matrix (ECM) patch for the delivery and retention of mesenchymal stem cells (MSC) for potential cardiac repair, using human bone marrow MSC at various cell densities under standard, static cell culture conditions .…”

Section: Tissue‐based Biomaterialsmentioning

confidence: 99%

“…Another key aspect is that it still provides a cell friendly environment . Thus, a Cardiocel™ product has been examined as a natural extracellular matrix (ECM) patch for the delivery and retention of mesenchymal stem cells (MSC) for potential cardiac repair, using human bone marrow MSC at various cell densities under standard, static cell culture conditions . This study showed good cell attachment and viability followed by good proliferation.…”

Section: Tissue‐based Biomaterialsmentioning

confidence: 99%

Biomedical applications of collagens

Ramshaw

2015

J Biomed Mater Res

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Collagen-based biomedical materials have developed into important, clinically effective materials used in a range of devices that have gained wide acceptance. These devices come with collagen in various formats, including those based on stabilized natural tissues, those that are based on extracted and purified collagens, and designed composite, biosynthetic materials. Further knowledge on the structure and function of collagens has led to on-going developments and improvements. Among these developments has been the production of recombinant collagen materials that are well defined and are disease free. Most recently, a group of bacterial, non-animal collagens has emerged that may provide an excellent, novel source of collagen for use in biomaterials and other applications. These newer collagens are discussed in detail. They can be modified to direct their function, and they can be fabricated into various formats, including films and sponges, while solutions can also be adapted for use in surface coating technologies.

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Impact of Detergent-Based Decellularization Methods on Porcine Tissues for Heart Valve Engineering

et al. 2016

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To date an optimal decellularization protocol of heart valve leaflets (HVL) and pericardia (PER) with an adequate preservation of the extracellular matrix (ECM) is still lacking. This study compares a 4 day Triton X-100-based protocol with faster SDC-based protocols for the decellularization of cardiac tissues. Decellularized and non-treated HVL and PER were processed for histological, biochemical and mechanical analysis to determine the effect of these agents on the structure, ECM components, and biomechanical properties. Tissues treated with SDC-based protocols still showed nuclear material, whereas tissues treated with Triton X-100 1% + ENZ ± TRYP were completely cell free. For both decellularized tissues, an almost complete washout of glycosaminoglycans, a reduction of soluble collagen and an alteration of the surface ultrastructure was observed. Interestingly, only the elastic fibers of pericardial tissue were affected and this tissue had a decreased maximum load. This study showed that both detergents had a similar impact on the ECM. However, Triton X-100 1% +DNase/RNase (ENZ) ± Trypsin (TRYP) is the only protocol that generated completely cell free bioscaffolds. Also, our study clearly demonstrated that the decellularization agents have more impact on pericardial tissues than on heart valve leaflets. Thus, for the purpose of tissue engineering of heart valves, it is advisable to use valvular rather than pericardial matrices.

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Evaluation of an established pericardium patch for delivery of mesenchymal stem cells to cardiac tissue

Cited by 6 publications

References 0 publications

The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

The component leaching from decellularized pericardial bioscaffolds and its implication in the macrophage response

Biomedical applications of collagens

Impact of Detergent-Based Decellularization Methods on Porcine Tissues for Heart Valve Engineering

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