Evaluation of an Enzyme-Linked Immunosorbent Assay Based on Araraquara Virus Recombinant Nucleocapsid Protein

Figueiredo, Luíz Tadeu Moraes; Moreli, Marcos Lázaro; Borges, Alessandra Abel; Figueiredo, Glauciane Garcia de; Badra, Soraya Jabur; Bisordi, Ivani; Suzuki, Akemi; Capria, Silvana; Padula, Paula

doi:10.4269/ajtmh.2009.81.273

Cited by 55 publications

(72 citation statements)

References 5 publications

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“…Numerous diagnostic methods for these viruses have been produced, among these, the production of recombinant proteins, which are used as antigen in ELISA to enable detection of specific antibodies against this virus, should be highlighted 10,11 .…”

Section: Souza Wm Et Al -Human Hantavirus Serosurvey On the Brazil Armentioning

confidence: 99%

Serosurvey of hantavirus infection in humans in the border region between Brazil and Argentina

Souza

Machado

Figueiredo

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: According to reports by the Ministry of Health, in the far western region of the State of Santa Catarina, there have been no reports of hantavirus pulmonary syndrome, a zoonotic disease transmitted by feces of infected rodents. A seroepidemiological study of residents of this region, was conducted, with the aim of determining the presence of hantavirus infections. A total of 340 volunteers of both genus, from the towns of Belmonte and Paraíso, were studied. Methods: The serum of these patients was collected and used to detect IgG antibodies against recombinant N protein of Araraquara hantavirus, by ELISA assay. The positive samples were then titrated and confirmed by immunofluorescence assay. Results: This study demonstrated the presence of IgG antibodies against hantavirus N protein in 3.5% of the population. The most frequent occupation was farm worker, 81% had direct and indirect contact with rodents, 91.7% of positive cases were farm workers, indicating that the probable cause of infection occurred during barn cleaning. These antibodies are noteworthy, given that the levels of antibodies were verified in individuals whose contact with hantavirus may have occurred many years ago. Conclusions: This study shows the circulation of hantavirus in the region, a fact that until now, had not reported. All the serum reagents had contact with the pathogen, but did not develop pulmonary and cardiovascular syndrome. It is important to remain alert, because hantavirus is a serious and emerging disease of some relevance. Keywords: Hantavirus. Seroprevalence in Santa Catarina. Morbidity. RESUMO Introdução:De acordo com relatórios do Ministério da Saúde, na região do extremo oeste do Estado de Santa Catarina, não há relatos de síndrome pulmonar por hantavírus, doença zoonótica transmitida por excretas de roedores contaminados. Com a finalidade de demosntrar a infecção por hantavírus, um estudo soroepidemiológico de moradores da região foi realizado. Assim, foi estudado um total de 340 voluntários de ambos os gêneros, dos municípios de Belmonte e Paraíso. Métodos: O soro destes pacientes foi coletado e usado para a detecção de anticorpos IgG contra a proteína N recombinante de hantavírus Araraquara, pelo teste de ELISA. As amostras positivas foram tituladas e confirmadas por imunofluorescência indireta. Resultados: Este estudo demonstrou a presença de anticorpos IgG contra a proteína N hantavírus em 3,5% da população. A ocupação de lavrador foi a mais frequente, e 81% tiveram contato direto e indireto com os roedores, 91,7% dos casos positivos foram os agricultores, a causa provável da infecção foi através da limpeza de celeiros. Estes anticorpos são notáveis, dado que os níveis de anticorpos são encontrados nos indivíduos cujo contato com o hantavírus pode ter ocorrido há muitos anos. Conclusões: Este estudo mostra a circulação de hantavírus na região, um fato que até então não havia relatado, todos os reagentes soro tiveram contato com o patógeno, mas não desenvolveram a síndrome pulmonar e cardiovascu...

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Section: Souza Wm Et Al -Human Hantavirus Serosurvey On the Brazil Armentioning

confidence: 99%

Serosurvey of hantavirus infection in humans in the border region between Brazil and Argentina

Souza

Machado

Figueiredo

et al. 2011

Rev. Soc. Bras. Med. Trop.

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“…The sera were tested with enzyme-linked immunosorbent assay (ELISA) for the presence of immunoglobulin M (IgM) and immunoglobulins G (IgG) antibodies to hantavirus, by using the recombinant nucleocapsid (N) protein of the Araraquara virus (ARAV), according to the description of Figueiredo et al (3) . Samples with an optical density equal to or greater than the average of the negative controls plus three standard deviations at a dilution of 1:400 were considered positives.…”

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confidence: 99%

Serological evidence of hantavirus infection in an urban area in Mato Grosso State, Brazil

Vieira

Silva

Barreto

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction:In Brazil, Mato Grosso (MT) has the highest number of hantavirus cardiopulmonary syndrome cases. Our study aimed to identify anti-hantavirus antibodies in the sera of patients from Sinop, MT, presenting with acute febrile illness. Methods: A retrospective analysis of data for 198 sera samples assessed using enzyme-linked immunosorbent assay (ELISA) was conducted. Results: Immunoglobulins G (IgGs) against the hantavirus nucleoprotein were found in 13.6% of the tested sera. No sample had immunoglobulin M (IgM) antibodies to hantavirus. Seropositivity occurred mainly in female residents in urban areas who worked around the household. Conclusions: Our findings suggest circulation of hantavirus in Sinop.Keywords: Zoonosis. Serosurvey study. Hantavirus.Hantaviruses (genus Hantavirus, family Bunyaviridae) are emerging zoonotic viruses of great importance for public health. In South America, the rodents of the Sigmodontinae subfamily are reservoirs of these viruses. Some hantaviruses infect humans, causing either hemorrhagic fever with renal syndrome (HFRS) and/or hantavirus cardiopulmonary syndrome (HCPS) (1) .The first cases of HCPS diagnosed in Brazil occurred in 1993. Since then, more than 1,920 cases of hantavirus have been notified in all Brazilian regions, with a case fatality rate of up to 39.5%. Mato Grosso (MT) is the leading state for HCPS cases and accounts for 16.1% of the total notified cases in Brazil. In a previous study conducted in the municipality of Sinop, Mato Grosso State, we carried out an epidemiological surveillance for arboviruses in 198 sera samples from patients presenting with acute febrile illness and clinical-epidemiological diagnoses of dengue. The results showed 46 samples positive for arboviruses. However, it was intriguing that more than 70% of the patients did not have dengue or other arbovirus infections that were tested for (2) . To address the possibility of hantavirus infection, we investigated the presence of antibodies against the hantavirus nucleoprotein in these febrile patients.Sinop is a municipality located 503km from the capital Cuiabá, in a geographical transition zone between a savannah and rainforest. The city was founded in 1974 and currently has an estimated population of approximately 130,000 inhabitants with 17.1% of them living in rural areas. The economic activities include an intensive agriculture based on the grain production, animal husbandry, logging, and human services. All of the sera analyzed in this study were part of the serum bank of the Laboratory of Immunology and Molecular Biology of Universidade Federal de Mato Grosso (UFMT), Campus of Sinop, which have been previously described in detail (2) . At the time of blood collection, the patients filled out a questionnaire that contained their epidemiological data, sex, age, occupation, address and region of birth, as well as information about any contact with rural or forested areas, and contact with rodents. These data were collected to analyze the risk factors associated with infect...

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“…In this study, protocols of IgM-ELISA and IgG-ELISA were performed as previously described by Figueiredo et al, using the recombinant N protein of Araraquara hantavirus as antigen (ARAV rN) produced in E. coli 8 . Figueiredo et al, found that ELISA has high sensitivity (97.2%), specificity (100%), positive predictive value (100%), and negative predictive value (98.1%).…”

mentioning

confidence: 99%

Hantavirus infection in suspected dengue cases from State of Ceará, Brazil

Lima

Júnior

Oliveira

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Evaluation of an Enzyme-Linked Immunosorbent Assay Based on Araraquara Virus Recombinant Nucleocapsid Protein

Cited by 55 publications

References 5 publications

Serosurvey of hantavirus infection in humans in the border region between Brazil and Argentina

Serosurvey of hantavirus infection in humans in the border region between Brazil and Argentina

Serological evidence of hantavirus infection in an urban area in Mato Grosso State, Brazil

Hantavirus infection in suspected dengue cases from State of Ceará, Brazil

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