Evaluation of an artificial dermis full-thickness skin defect model in the rat

Matsui, Risako; Osaki, Ken-ichi; Konishi, Jun; Ikegami, Kazuhito; Koide, Mikio

doi:10.1016/0142-9612(96)84673-4

Cited by 37 publications

(22 citation statements)

References 26 publications

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“…Preclinical evaluation of acute wound healing includes animal models of full thickness skin defects, usually in the mouse and rat, 37,38 but also in large animals such as porcine. 39 Here we used rat skin defects to answer questions regarding the biocompatibility of the engineered material and, more importantly, to help elucidate the potential mechanisms of curcumin for accelerated wound healing before putting them into a clinical setting.…”

Section: Discussionmentioning

confidence: 99%

Nano-formulated curcumin accelerates acute wound healing through Dkk-1-mediated fibroblast mobilization and MCP-1-mediated anti-inflammation

et al. 2017

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Turmeric, a product of Curcuma longa, has a very long history of being used for the treatment of wounds in many Asian countries. Curcumin, the principal curcuminoid of turmeric, has recently been identified as a main mediator of turmeric's medicinal properties. However, the inherent limitations of the compound itself, such as hydrophobicity, instability, poor absorption and rapid systemic elimination, pose big hurdles for translation to wider clinical application. We present here an approach for engineering curcumin/gelatin-blended nanofibrous mats (NMs) by electrospinning to adequately enhance the bioavailability of the hydrophobic curcumin for wound repair. Curcumin was successfully formulated as an amorphous nanosolid dispersion and favorably released from gelatin-based biomimetic NMs that could be easily applied topically to experimental wounds. We show synergistic signaling by the released curcumin during the healing process: (i) mobilization of wound site fibroblasts by activating the Wnt signaling pathway, partly mediated through Dickkopf-related protein-1, and (ii) persistent inhibition of the inflammatory response through decreased expression of monocyte chemoattractant protein-1 by fibroblasts. With a combination of these effects, the curcumin/gelatin-blended NMs enhanced the regenerative process in a rat model of acute wounds, providing a method for translating this ancient medicine for use in modern wound therapy.

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Section: Discussionmentioning

confidence: 99%

Nano-formulated curcumin accelerates acute wound healing through Dkk-1-mediated fibroblast mobilization and MCP-1-mediated anti-inflammation

et al. 2017

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“…It has been reported that Terudermis enhances fibroblasts and capillary infiltration into the material and hyaluronic acid synthesis, whereas it is necessary to apply a split-thickness skin graft to attain epithelialization after dermal regeneration. 23 The differences observed between the two materials used in this study may arise from the difference between the materials in cell adhesion. Indeed, cell adhesion to poly(Pro-Hyp-Gly) is lower than that to bovine type I collagen (data not shown).…”

Section: Discussionmentioning

confidence: 95%

The biodegradability of poly(Pro‐Hyp‐Gly) synthetic polypeptide and the promotion of a dermal wound epithelialization using a poly(Pro‐Hyp‐Gly) sponge

Tanihara

Kajiwara

Ida

et al. 2007

J Biomedical Materials Res

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Collagens are widely used in medical applications, but animal-derived collagens have several drawbacks, such as low thermal stability, nonspecific cell attachment, and susceptibility to contamination by infectious pathogens, such as prions, which may transfect humans. We have previously reported the chemical synthesis of polypeptides consisting of a Pro-Hyp-Gly sequence and the high thermostability of their triple-helical structure. To clarify the biomaterial characteristics of the poly(Pro-Hyp-Gly) polypeptide, we assessed its biodegradability and its capability for skin regeneration. Eight weeks after implantation, a poly(Pro-Hyp-Gly) freeze-dried sponge embedded subcutaneously into a rat dorsal area degraded at the same rate as Terudermis, which is made from bovine type I atelocollagen and is used as an artificial dermis. Surprisingly, compared with Terudermis, the poly(Pro-Hyp-Gly) sponge significantly promoted epithelialization of a full-thickness wound on a rabbit's ear pad. This chemically synthesized polypeptide may be useful as a scaffold for tissue engineering and tissue regeneration.

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“…Following fixation, samples were dehydrated, and embedded in paraffin. Five micrometer microtomed sections of the skin tissue samples were stained with hematoxylin and eosin according to the common histological procedures (Matsui et al, 1996a,b). The stained slides were examined under an Olympus BX40 microscope and assessed for histo-pathological changes associated with chemical exposure.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of EpiDerm full thickness-300 (EFT-300) as an in vitro model for skin irritation: Studies on aliphatic hydrocarbons

Mallampati

Patlolla

Agarwal

et al. 2010

Toxicology in Vitro

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The aim of this study was to understand the skin irritation effects of saturated aliphatic hydrocarbons (HCs), C9–C16, found jet fuels using in vitro 3-dimensional EpiDerm full thickness-300 (EFT-300) skin cultures. The EFT-300 cultures were treated with 2.5 µl of HCs and the culture medium and skin samples were collected at 24 and 48 h to measure the release of various inflammatory biomarkers (IL-1α, IL-6 and IL-8). To validate the in vitro results, in vivo skin irritation studies were carried out in hairless rats by measuring trans epidermal water loss (TEWL) and erythema following un-occlusive dermal exposure of HCs for 72 h. The MTT tissue viability assay results with the EFT-300 tissue show that 2.5 µl/tissue (≈4.1 µl/cm2) of the HCs did not induce any significant changes in the tissue viability for exposure times up to 48 h of exposure. Microscopic observation of the EFT-300 cross-sections indicated that there were no obvious changes in the tissue morphology of the samples at 24 h, but after 48 h of exposure, tridecane, tetradecane and hexadecane produced a slight thickening and disruption of stratum corneum. Dermal exposures of C12–C16 HCs for 24 h significantly increased the expression of IL-1α in the skin as well as in the culture medium. Similarly, dermal exposure of all HCs for 24 h significantly increased the expression of interleukin-6 (IL-6) and IL-8 in the skin as well as in the culture medium in proportion to the HC chain length. As the exposure time increased to 48 h, IL-6 concentrations increased 2-fold compared to the IL-6 values at 24 h. The in vivo skin irritation data also showed that both TEWL and erythema scores increased with increased HCs chain length (C9–C16). In conclusion, the EFT-300 showed that the skin irritation profile of HCs was in the order of C9 ≤ C10 ≤ C11 ≤ C12 < C13 ≈ C14 ≈ C16 and that the tissue was an excellent in vitro model to predict in vivo irritation and to understand the structural activity relationship of HCs.

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Evaluation of an artificial dermis full-thickness skin defect model in the rat

Cited by 37 publications

References 26 publications

Nano-formulated curcumin accelerates acute wound healing through Dkk-1-mediated fibroblast mobilization and MCP-1-mediated anti-inflammation

Nano-formulated curcumin accelerates acute wound healing through Dkk-1-mediated fibroblast mobilization and MCP-1-mediated anti-inflammation

The biodegradability of poly(Pro‐Hyp‐Gly) synthetic polypeptide and the promotion of a dermal wound epithelialization using a poly(Pro‐Hyp‐Gly) sponge

Evaluation of EpiDerm full thickness-300 (EFT-300) as an in vitro model for skin irritation: Studies on aliphatic hydrocarbons

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