Evaluation of a Thermoresponsive Polycaprolactone Scaffold for <i>In Vitro</i> Three-Dimensional Stem Cell Differentiation

Hruschka, Veronika; Saeed, Aram; Slezak, Paul; Ghanami, Racha Cheikh Al; Feichtinger, Georg A.; Alexander, Cameron; Redl, Heinz; Shakesheff, Kevin M.; Wolbank, Susanne

doi:10.1089/ten.tea.2013.0710

Cited by 11 publications

(5 citation statements)

References 51 publications

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“…The longer-term culture experiments thus demonstrated that the surfaces were able to function as 'reversible attachment' cell support matrices over timescales generally used for cell expansion. The utility of switchable attachment to polymer surfaces for cell culture has been extensively tested over recent years, 31,32 but the ability to culture cells at lower temperature and then release the cells by heating to body temperature may offer advantages for more thermally sensitive cell types. Overall, these results are significant also because they demonstrate a mechanism underlying the cell adhesion phenomenon.…”

Section: Resultsmentioning

confidence: 99%

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

et al. 2017

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We report the synthesis of thermo-responsive polymer brushes with Upper Critical Solution Temperature (UCST)-type behaviour on glass to provide a new means to control cell attachment. Thermoresponsive poly(N-acryloyl glycinamide)-statpoly(N-phenylacrylamide) (PNAGAm-PNPhAm) brushes with three different monomer ratios were synthesized to give tunable phase transition temperatures (Tp) in solution. Surface energies of surface-grafted brushes of these polymers at 25, 32, 37 and 50 C were calculated from contact angle measurements and atomic force microscopy (AFM) studies confirmed that these polymers were highly extended at temperatures close to Tp in physiologically-relevant media. Importantly, NIH-3T3 cells were attached on the collapsed PNAGAm-PNPhAm brush surface at 30 C after 20 h incubation, while release of cells from the extended brushes was observed within 2 h after the culture temperature was switched to 37 C. Furthermore, the changes in cell attachment followed changes in the Lewis base component of surface energy. The results indicate that, in contrast to the established paradigm of enhanced cell attachment to surfaces where polymers are above a Lower Critical Solution Temperature (LCST), these novel substrates enable detachment of cells from surfaces at temperatures above a UCST. In turn these responsive materials open new avenues for the use of polymer-modified surfaces to control cell attachment for applications in cell manufacture and regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

et al. 2017

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“…Poly(α‐esters) are normally synthesized by poly‐condensation or ring‐opening‐polymerization (ROP) from a variety of monomers. Poly(lactic acid) (PLA) and poly(caprolactone) (PCL) have been intensively investigated for biomedical applications, They are approved for pharmaceutical use and have been widely used in the fields of controlled drug release and tissue engineering. Their copolymers can be produced from relatively cheap monomers which can be readily metabolized.…”

Section: Introductionmentioning

confidence: 99%

Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles

Taresco

Suksiriworapong

Creasey

et al. 2016

J. Polym. Sci. Part A: Polym. Chem.

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There is an increasing need to develop bio‐compatible polymers with an increased range of different physicochemical properties. Poly(glycerol‐adipate) (PGA) is a biocompatible, biodegradable amphiphilic polyester routinely produced from divinyl adipate and unprotected glycerol by an enzymatic route, bearing a hydroxyl group that can be further functionalized. Polymers with an average Mn of ∼13 kDa can be synthesized without any post‐polymerization deprotection reactions. Acylated polymers with fatty acid chain length of C4, C8, and C18 (PGAB, PGAO, and PGAS, respectively) at different degrees of substitution were prepared. These modifications yield comb‐like polymers that modulate the amphiphilic characteristics of PGA. This novel class of biocompatible polymers has been characterized through various techniques such as FT‐IR, 1H NMR, surface, thermal analysis, and their ability to self‐assemble into colloidal structures was evaluated by using DLS. The highly tunable properties of PGA reported herein demonstrate a biodegradable polymer platform, ideal for engineering solid dispersions, nanoemulsions, or nanoparticles for healthcare applications. © 2016 The Authors. Journal of Polymer Science Part A: Polymer Chemistry Published by Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 3267–3278

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“…For example, thermo-responsive polymers with different chemistries have been successfully used to create contiguous intact constructs using numerous cell types, including corneal [5], skin [6], oral [7], and nasal epithelial cells [8], cardiomyocytes [9], muscle myoblasts [10], and mesenchymal stem cells [11]. This is evidently an attractive bottom-up strategy, as it not only provides cells with a template to produce native-like tissues but also ensures that, by the end of the bio-fabrication process, the tissue is dissociated from the template.…”

Section: Introductionmentioning

confidence: 99%

Bio-fabrication and physiological self-release of tissue equivalents using smart peptide amphiphile templates

Gouveia

Hamley

Connon

2015

J Mater Sci: Mater Med

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In this study we applied a smart biomaterial formed from a self-assembling, multi-functional synthetic peptide amphiphile (PA) to coat substrates with various surface chemistries. The combination of PA coating and alignment-inducing functionalised substrates provided a template to instruct human corneal stromal fibroblasts to adhere, become aligned and then bio-fabricate a highly-ordered, multi-layered, three-dimensional tissue by depositing an aligned, native-like extracellular matrix. The newly-formed corneal tissue equivalent was subsequently able to eliminate the adhesive properties of the template and govern its own complete release via the action of endogenous proteases. Tissues recovered through this method were structurally stable, easily handled, and carrier-free. Furthermore, topographical and mechanical analysis by atomic force microscopy showed that tissue equivalents formed on the alignment-inducing PA template had highly-ordered, compact collagen deposition, with a two-fold higher elastic modulus compared to the less compact tissues produced on the non-alignment template, the PA-coated glass. We suggest that this technology represents a new paradigm in tissue engineering and regenerative medicine, whereby all processes for the bio-fabrication and subsequent self-release of natural, bio-prosthetic human tissues depend solely on simple template-tissue feedback interactions.

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Evaluation of a Thermoresponsive Polycaprolactone Scaffold for In Vitro Three-Dimensional Stem Cell Differentiation

Cited by 11 publications

References 51 publications

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles

Bio-fabrication and physiological self-release of tissue equivalents using smart peptide amphiphile templates

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