Evaluation of a Rapid Oral Bacteria Quantification System Using Dielectrophoresis and the Impedance Measurement

Hirota, Kaoru; Inagaki, Shigenori; Hamada, Ryo; Ishihara, Kazuyuki; Miyake, Yoichiro

doi:10.4265/bio.19.45

Cited by 49 publications

(25 citation statements)

References 20 publications

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“…Other approaches to rapidly measuring oral bacteria have been developed for or applied to oral health questions, including the dielectrophoretic impedance measurement where a strong correlation between signal and bacterial abundance has been reported (Hirota et al ) and methods involving the detection of ATP (Iwawaki et al ).…”

Section: Discussionmentioning

confidence: 99%

A rapid chair‐side method for the estimation of oral bacterial colonization density

et al. 2019

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Aims Caries and periodontal disease are associated with inadequate control of oral bacteria. Since conventional microbiological evaluations are impractical in dental clinics or public engagement activities, a rapid test for the quantification of oral bacteria represents a useful tool. We describe the development of a colour change test to rapidly estimate bacterial colonisation density in the mouth. Methods and Results Volunteers rinsed with milk or milkshake. Viability indicators were added and colour changes quantified during incubation. Using milkshake and the resazurin‐based solution PrestoBlue (9% v/v), the method distinguished between samples before and after brushing within 5 min. Colour changes were quantified and viable counts were obtained using oral rinses. Measured colour changes strongly correlated with total counts of both anaerobes and streptococci (Spearman’s correlation coefficient of 0·782 and 0·769, respectively, P ≤ 0·001) and with perceived changes, as determined by volunteers (n = 10) visually ranking images. Conclusions The resazurin milkshake test can rapidly and visually quantify viable bacteria in oral samples. Significance and Impact of the Study The resazurin milkshake test could serve as a sensitive semi‐quantitative method for measuring oral bacteria in human oral rinse samples.

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Section: Discussionmentioning

confidence: 99%

A rapid chair‐side method for the estimation of oral bacterial colonization density

et al. 2019

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“…The original, commercially available DEPIM apparatus requires 20 s for a measurement of total bacteria present in the sample solution [15]. The DEPIM method has been shown to be effective in measuring total bacterial numbers in a mixed oral bacterial suspension, and is unaffected by bacterial aggregates [22].…”

Section: Measurement With Antigeneantibody Reaction Assisted-depim Apmentioning

confidence: 99%

Point-of-care detection of Tannerella forsythia using an antigen–antibody assisted dielectrophoretic impedance measurement method

Ishii

Imamura

Kikuchi

et al. 2015

Microbial Pathogenesis

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“…The concentration of total bacteria in the oropharyngeal fluid was determined using the Rapid Oral Bacteria Quantification System (Panasonic Healthcare Co. Ltd., Osaka, Japan), which is based on dielectrophoresis and impedance measurements [8,9]. To collect the samples, a cotton swab was immersed in oropharyngeal fluid for 5 s before oral care and 1 min, 1 h, 2 h, and 3 h after oral care.…”

Section: Consent For Publication Interventionmentioning

confidence: 99%

Topical povidone iodine inhibits bacterial growth in the oral cavity of patients on mechanical ventilation: a randomized controlled study

et al. 2020

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Background: Topical 0.12% chlorhexidine has been used widely to prevent ventilator-associated pneumonia in patients undergoing mechanical ventilation. However, it is not approved for mucosal application in Japan. The aims of this study were to investigate if topical povidone iodine (i) inhibits bacterial growth and (ii) disrupts the balance of the oral microbiota. Methods: This randomized controlled clinical trial included 23 patients who underwent mechanical ventilation in the intensive care unit. The patients were divided randomly into two groups: the intervention group (n = 16) and the control group (n = 7). All patients received oral cleaning with 3% hydrogen peroxide, followed by irrigation with tap water. The patients in the intervention group received 10% povidone iodine applied topically to the oral cavity. The concentration of total bacteria in the oropharyngeal fluid were determined before, immediately after, 1 h, 2 h, and 3 h after oral care using the Rapid Oral Bacteria Quantification System, which is based on dielectrophoresis and impedance measurements. The number of streptococci, methicillin-resistant Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, Porphyromonas gingivalis, and Candida albicans before, immediately after, 1 h, and 3 h after oral care were estimated based on real-time polymerase chain reaction data. Results: After irrigation of the oral cavity, the number of bacteria decreased, but increased again at 1 h after oral care in the control group; however, in the intervention group, the concentration of bacteria was significantly lower than that in the control group at 1 hour (p = 0.009), 2 h (p = 0.001), and 3 h (p = 0.001) after oral care. The growth of all bacterial species tested was inhibited in the intervention group at 3 h after oral care, suggesting that povidone iodine did not disturb the balance of the oral microbiota.Conclusions: Topical application of povidone iodine after cleaning and irrigation of the oral cavity inhibited bacterial growth in the oropharyngeal fluid of patients on mechanical ventilation while not disrupting the balance of the oral microbiota.

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Evaluation of a Rapid Oral Bacteria Quantification System Using Dielectrophoresis and the Impedance Measurement

Cited by 49 publications

References 20 publications

A rapid chair‐side method for the estimation of oral bacterial colonization density

A rapid chair‐side method for the estimation of oral bacterial colonization density

Point-of-care detection of Tannerella forsythia using an antigen–antibody assisted dielectrophoretic impedance measurement method

Topical povidone iodine inhibits bacterial growth in the oral cavity of patients on mechanical ventilation: a randomized controlled study

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