Evaluation of a PCR–RFLP–ITS2 assay for discrimination of Anopheles species in northern and western Colombia

Cienfuegos-Gallet, Astrid V.; Rosero-García, Doris; Naranjo-Díaz, Nelson; Luckhart, Shirley; Conn, Jan E.; Correa, Margarita M.

doi:10.1016/j.actatropica.2011.02.004

Cited by 44 publications

(51 citation statements)

References 29 publications

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“…triannulatus s.l. from NW Colombia, 15,16 with three bands (226, 180, and 156 bp), and was present in both the NW and SE regions ( Figure 2, Table 1). Pattern II of 198, 180, and 155 bp, was found in specimens of the three SE localities (Figure 2, Table 1), however, one specimen from TAR showed a pattern of 218, 177, and 155 bp that, when compared with the larger NW sequences, reveled several deletions and one nucleotide insertion (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…triannulatus s.l. 15,16 By PCR-RFLP-ITS2 or COI analysis, it was not possible to determine whether An. triannulatus s.l.…”

Section: Discussionmentioning

confidence: 99%

“…specimens from other NW localities. 16 These results suggested that the PCR-RFLP-ITS2 assay could be used to differentiate An. triannulatus s.l.…”

Section: Introductionmentioning

confidence: 96%

“…by a PCR-RFLP-ITS2 assay. 15,16 Furthermore, an in silico AluI restriction enzyme analysis using NEBcutter software 23 was performed using 10 ITS2 sequences obtained in this work representative of NW and SE specimens, three from Meta-MET Department available in GenBank (accession nos. HM022422, HM022423, and HM022426) and one sequence from Brazil (accession no.…”

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confidence: 99%

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Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Rosero-García¹,

Jaramillo²,

Gutiérrez³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Anopheles triannulatus s.l. is a species complex, however in Colombia its taxonomic status is unclear. This study was conducted to understand the level of genetic differentiation or population structure of specimens of An. triannulatus s.l. from northwestern and southeastern Colombia. Cytochrome oxidase subunit I (COI) and internal transcribed spacer (ITS2) sequence analyses suggested high genetic differentiation between the NW and SE populations. A TCS network and Bayesian inference analysis based on 814 bp of COI showed two main groups: group I included samples from the NW and group II samples from the SE. Two main ITS2-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) patterns were found. Pattern I is present in both the NW and SE, and pattern II is found in the SE specimens. To further elucidate the taxonomic status of An. triannulatus s.l. in Colombia and how these COI lineages are related to the Triannulatus Complex species, the evaluation of immature stages, male genitalia, and additional mitochondrial and nuclear markers will be needed.

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Section: Resultsmentioning

confidence: 99%

“…triannulatus s.l. 15,16 By PCR-RFLP-ITS2 or COI analysis, it was not possible to determine whether An. triannulatus s.l.…”

Section: Discussionmentioning

confidence: 99%

“…specimens from other NW localities. 16 These results suggested that the PCR-RFLP-ITS2 assay could be used to differentiate An. triannulatus s.l.…”

Section: Introductionmentioning

confidence: 96%

mentioning

confidence: 99%

See 2 more Smart Citations

Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Rosero-García¹,

Jaramillo²,

Gutiérrez³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…benarrochi s.l. and other closely related species and can compromise vector control strategies 12,13,19,28,29 ; in contrast, ITS2 is generally reliable. 27,30,31 All specimens collected were tested for the presence of Plasmodium using CytB-PCR but none were positive.…”

Section: Introductionmentioning

confidence: 99%

Molecular Taxonomy of Anopheles (Nyssorhynchus) benarrochi (Diptera: Culicidae) and Malaria Epidemiology in Southern Amazonian Peru

Conn

Moreno²,

Saavedra³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Anopheline specimens were collected in 2011 by human landing catch, Shannon and CDC traps from the malaria endemic localities of Santa Rosa and San Pedro in Madre de Dios Department, Peru. Most specimens were either Anopheles (Nyssorhynchus) benarrochi B or An. (Nys.) rangeli, confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (PCR-RFLP-ITS2) and, for selected individuals, ITS2 sequences. A few specimens from Lupuna, Loreto Department, northern Amazonian Peru, were also identified as An. benarrochi B. A statistical parsimony network using ITS2 sequences confirmed that all Peruvian An. benarrochi B analyzed were identical to those in GenBank from Putumayo, southern Colombia. Sequences of the mtDNA COI BOLD region of specimens from all three Peruvian localities were connected using a statistical parsimony network, although there were multiple mutation steps between northern and southern Peruvian sequences. A Bayesian inference of concatenated Peruvian sequences of ITS2+COI detected a single clade with very high support for all An. benarrochi B except one individual from Lupuna that was excluded. No samples were positive for Plasmodium by CytB-PCR.

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Amazonian malaria: Asymptomatic human reservoirs, diagnostic challenges, environmentally driven changes in mosquito vector populations, and the mandate for sustainable control strategies

et al. 2012

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Across the Americas and the Caribbean, nearly 561,000 slide-confirmed malaria infections were reported officially in 2008. The nine Amazonian countries accounted for 89% of these infections; Brazil and Peru alone contributed 56% and 7% of them, respectively. Local populations of the relatively neglected parasite P. vivax, which currently accounts for 77% of the regional malaria burden, are extremely diverse genetically and geographically structured. At a time when malaria elimination is placed on the public health agenda of several endemic countries, it remains unclear why malaria proved so difficult to control in areas of relatively low levels of transmission such as the Amazon Basin. We hypothesize that asymptomatic parasite carriage and massive environmental changes that affect vector abundance and behavior are major contributors to malaria transmission in epidemiologically diverse areas across the Amazon Basin. Here we review available data supporting this hypothesis and discuss their implications for current and future malaria intervention policies in the region. Given that locally generated scientific evidence is urgently required to support malaria control interventions in Amazonia, we briefly describe the aims of our current field-oriented malaria research in rural villages and gold-mining enclaves in Peru and a recently opened agricultural settlement in Brazil.

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Evaluation of a PCR–RFLP–ITS2 assay for discrimination of Anopheles species in northern and western Colombia

Cited by 44 publications

References 29 publications

Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Genetic Diversity of Anopheles triannulatus s.l. (Diptera: Culicidae) from Northwestern and Southeastern Colombia

Molecular Taxonomy of Anopheles (Nyssorhynchus) benarrochi (Diptera: Culicidae) and Malaria Epidemiology in Southern Amazonian Peru

Amazonian malaria: Asymptomatic human reservoirs, diagnostic challenges, environmentally driven changes in mosquito vector populations, and the mandate for sustainable control strategies

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