Evaluation of a human ovarian follicle isolation technique to obtain disease-free follicle suspensions before safely grafting to cancer patients

Soares, Michelle; Sahrari, Karima; Amorim, Christiani Andrade; Saussoy, Pascale; Donnez, Jacques; Dolmans, Marie-Madeleine

doi:10.1016/j.fertnstert.2015.05.021

Cited by 48 publications

(48 citation statements)

References 53 publications

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“…The cryopreserved tissue may contain cancer cells, such as those present within the circulation (i.e., circulating tumor cells). In the particular case of leukemia, more than 50% of cryopreserved ovarian tissue can contain leukemic cells (Soares et al, 2015). The reseeding of disease has been observed clinically with ovarian tissue transplantation (Dolmans et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The reseeding of disease has been observed clinically with ovarian tissue transplantation (Dolmans et al, 2013). Protocols are being developed to rid ovarian tissue of cancerous cells (Soares et al, 2015), but have not been widely adopted for clinical use, which underscores the need to develop technologies that prevent re-seeding of cancer cells. Isolation of transplanted follicles from host tissue has the potential to substantially reduce the number of cancer cells in the graft (Donnez et al, 2011; Kniazeva et al, 2015), yet follicle isolation may not be able to remove the presence of all tumor cells in every graft.…”

Section: Discussionmentioning

confidence: 99%

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Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Rios

Kniazeva

Lee

et al. 2018

Biotech & Bioengineering

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Cancer survivorship rates have drastically increased due to improved efficacy of oncologic treatments. Consequently, clinical concerns have shifted from solely focusing on survival to quality of life, with fertility preservation as an important consideration. Among fertility preservation strategies for female patients, ovarian tissue cryopreservation and subsequent reimplantation has been the only clinical option available to cancer survivors with cryopreserved tissue. However, follicle atresia after transplantation and risk of reintroducing malignant cells have prevented this procedure from becoming widely adopted in clinics. Herein, we investigated the encapsulation of ovarian follicles in alginate hydrogels that isolate the graft from the host, yet allows for maturation after transplantation at a heterotopic (i.e., subcutaneous) site, a process we termed in vivo follicle maturation. Survival of multiple follicle populations was confirmed via histology, with the notable development of the antral follicles. Collected oocytes (63%) exhibited polar body extrusion and were fertilized by intracytoplasmic sperm injection and standard in vitro fertilization procedures. Successfully fertilized oocytes developed to the pronucleus (14%), two-cell (36%), and four-cell (7%) stages. Furthermore, ovarian follicles cotransplanted with metastatic breast cancer cells within the hydrogels allowed for retrieval of the follicles, and no mice developed tumors after removal of the implant, confirming that the hydrogel prevented seeding of disease within the host. Collectively, these findings demonstrate a viable option for safe use of potentially cancer-laden ovarian donor tissue for in vivo follicle maturation within a retrievable hydrogel and subsequent oocyte collection. Ultimately, this technology may provide novel options to preserve fertility for young female patients with cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Rios

Kniazeva

Lee

et al. 2018

Biotech & Bioengineering

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“…Indeed, it cannot be excluded that some malignant cells could contaminate isolated follicle suspensions obtained from patients with leukemia. Based on this assumption, Soares et al [61] reported a technique to effectively eliminate cancer cells from the follicle suspension without altering follicle viability. They found that in case of contamination of ovarian tissue by malignant cells, there is a real risk of picking up cancer cells along with isolated follicles during follicle retrieval.…”

Section: Other Important Aspects To Consider: Safetymentioning

confidence: 99%

“…However, this risk is dramatically reduced when a simple mechanical procedure is added: manual pipetting of follicles, with transfer to fresh medium droplets, in order to remove unwanted contaminating cells. Repeating this procedure three times led to successful elimination of leukemic cells from the follicle suspension [61].…”

Section: Other Important Aspects To Consider: Safetymentioning

confidence: 99%

“…Because Soares et al [61] detected leukemic cell contamination of the follicle suspension after pickup, they conducted another study to investigate the leukemiainducing potential of a few leukemic cells grafted inside an artificial ovary environment using an immunodeficient mouse xenografting model [62]. Leukemic cells were embedded in a fibrin matrix along with ovarian cells in order to study leukemic cell survival and proliferation in conditions resembling the artificial ovary environment as closely as possible.…”

Section: Other Important Aspects To Consider: Safetymentioning

confidence: 99%

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Artificial Ovary

Amorim¹

2016

Gonadal Tissue Cryopreservation in Fertility Preservation

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Survival rates of many malignant diseases are steadily improving, but for patients of childbearing age, fertility restoration often becomes a vital concern after disease remission. In women, treatments such as chemo/radiotherapy can be very harmful to the ovaries, causing loss of both endocrine and reproductive functions. When gonadotoxic treatment cannot be delayed, ovarian tissue cryobanking is the only way of preserving fertility. However, this technique is not advisable for patients with certain types of cancer, since there is a risk of reintroducing malignant cells present in the cryopreserved tissue. For these patients, a safer alternative could be transplantation of isolated preantral follicles back to their natural environment. To encapsulate and protect isolated follicles, a transplantable artificial ovary needs to be created. The main goal of the artificial ovary is to mimic the natural organ, and for this, it should be composed of a matrix that encapsulates and protects not only the isolated follicles but also autologous ovarian cells and bioactive factors, which are necessary for follicle survival and development. The aim of this chapter is to describe this new technology, its indications, advantages, and the different approaches to create it.

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Further Fertility Preservation Techniques

Dittrich

Wolff

2020

Fertility Preservation in Oncological and Non-Oncological Diseases

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Evaluation of a human ovarian follicle isolation technique to obtain disease-free follicle suspensions before safely grafting to cancer patients

Cited by 48 publications

References 53 publications

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Artificial Ovary

Further Fertility Preservation Techniques

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