Evaluation of a Commercially Available Human Serum Amyloid A (SAA) Turbidimetric Immunoassay for Determination of Feline SAA Concentration

Hansen, A E; Schaap, M K; Kjelgaard‐Hansen, Mads

doi:10.1007/s11259-006-3373-6

Cited by 53 publications

(67 citation statements)

References 14 publications

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“…In this study, values of SAA lower than the detection limit (DL) of the assay (0.38 μg/mL) were set as equal to DL for statistical analysis. It should be pointed out that this approach could have resulted in bias in the statistical results.…”

Section: Discussionmentioning

confidence: 99%

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Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours

Vilhena

Tvarijonaviciute

Cerón

et al. 2019

Vet Comparative Oncology

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Acute phase proteins (APP) and biomarkers of oxidative status change in human and canine mammary tumours, however, they have not been studied in feline mammary tumours. The aims of this study were to investigate the APP and antioxidant responses in feline malignant mammary tumours, to evaluate their relation with tumour features, and to assess their prognostic value. Serum amyloid A (SAA), haptoglobin (Hp), albumin, butyrylcholinesterase (BChE), insulin‐like growth factor1 (IGF1), paraoxonase1 (PON1), total serum thiols (Thiol), glutathione peroxidase (GPox) and total antioxidant capacity determined by different assays, including trolox equivalent antioxidant capacity assessed by two different methodologies (TEAC1/2), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC), were determined in serum of 50 queens with spontaneous mammary carcinomas and of 12 healthy female cats. At diagnosis, diseased queens presented significantly higher SAA and Hp, and lower albumin, BChE, GPox, TEAC1, TEAC2 and CUPRAC than controls. Different tumour features influenced concentrations of APP and antioxidants. Increases in serum Hp, and decreases in albumin, Thiol and FRAP were significantly associated with neoplastic vascular emboli, metastasis in regional lymph nodes and/or in distant organs. Distant metastasis development during the course of the disease was associated with increases in SAA and TEAC1. At diagnosis, decreased albumin was associated with a longer survival, and BChE <1.15 μmoL/mL.minute was associated with a shorter survival time on multivariate analysis. Feline malignant mammary tumours are associated with an APP response and oxidative stress, and different tumour features influence the inflammatory response and the oxidative damage. Furthermore, some of these analytes proved to have prognostic value.

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Section: Discussionmentioning

confidence: 99%

“…SAA concentrations were determined by a human turbidimetric immunoassay (LZ‐SAA; Eiken Chemical Co., Tokyo, Japan) previously validated for use in cats . Serum Hp concentrations were determined by use of the haemoglobin‐binding method with the use of a commercial kit (Tridelta Development Ltd., Kildare, Ireland).…”

Section: Methodsmentioning

confidence: 99%

Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours

Vilhena

Tvarijonaviciute

Cerón

et al. 2019

Vet Comparative Oncology

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“…Most methods are timeconsuming, however, and relatively expensive, so limiting the wide-scale use of APPs in routine practice. Recently, immunoturbidimetric tests for measuring feline APPs have been validated (Bence et al, 2005;Hansen et al, 2006) and should probably reduce the cost per test, thus allowing greater use of APPs measurement.…”

Section: How To Measure Appsmentioning

confidence: 99%

The feline acute phase reaction

Paltrinieri¹

2008

The Veterinary Journal

131

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The acute phase reaction (APR) is a response to potentially pathogenic stimuli. It begins with the release of interleukin (IL)-1, IL-6 and tumour necrosis factor (TNF)-alpha from inflammatory cells. These cytokines induce fever, leucocytosis and release of serum acute phase proteins (APPs). In this review, the characteristics of the feline APR are described. In cats with inflammatory conditions, fever is a common finding, with leucocytosis due to the release of cells from the marginal pool, followed by activation of myelopoiesis. Because excitement frequently causes leucocytosis in cats, a diagnosis of inflammation should therefore be supported by additional findings such as the presence of toxic neutrophils. The major APPs are serum amyloid A and alpha(1)-acid glycoprotein (AGP), which both increase a few hours after the inflammatory stimulus and remain elevated for as long as the inflammation persists. AGP plays an important role in the diagnosis of feline infectious peritonitis (FIP) and may also be useful also in studies of FIP pathogenesis.

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“…In 2006, Hansen et al reported that the SAA concentration in cats could be measured using a commercially available turbidimetric immunoassay (TIA) designed for measuring human SAA [8]. The purposes of this study were to assess the applicability of human SAA-TIA to measurement of the feline SAA concentration by using recombinant feline SAA protein (rfSAA) and to evaluate the clinical usefulness of measuring the SAA concentration routinely in cats with various diseases.…”

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confidence: 99%

Verification of Measurement of the Feline Serum Amyloid A (SAA) Concentration by Human SAA Turbidimetric Immunoassay and Its Clinical Application

Tamamoto

Ohno

Ohmi

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Serum amyloid A (SAA) is one of the major acute phase proteins in cats that has potential to be used as an inflammatory marker. A previous study showed that the human SAA turbidimetric immunoassay (hSAA-TIA) could be used to measure the SAA concentration in cats. The objectives of the present study were to assess use of hSAA-TIA for determining the feline SAA concentration and to evaluate its clinical application. Recombinant feline SAA protein (rfSAA) was expressed in Escherichia coli and purified for SDS-PAGE and immunoblot analysis with anti-human SAA antibodies. The concentration of rfSAA was determined by ELISA and hSAA-TIA. Plasma SAA concentrations were measured in healthy and diseased cats by hSAA-TIA. The time-courses changes in the SAA and α1-acid glycoprotein (AGP) concentrations in the cats after ovariohysterectomy were investigated. In SDS-PAGE, rfSAA was detected as a clear band that reacted with anti-human SAA antibodies. There was significant correlation between the SAA concentration measured by ELISA and hSAA-TIA. The SAA concentration of the diseased cats (n=263) was significantly increased (P<0.01; 0.0-88.9 mg/l, mean: 7.52 mg/l) compared with that in the healthy cats (n=26; 0.0-0.9 mg/l, mean: 0.14 mg/l). No correlation was observed between SAA and WBC in the diseased cats. The SAA concentration changed more rapidly and remarkably than the AGP concentration after ovariohysterectomy. The present study revealed that hSAA-TIA is useful for determination of the feline SAA concentration. Measurement of the SAA concentration, in addition to the WBC count, would be clinically valuable as a routine test to detect inflammation. KEY WORDS: acute phase protein, α1-acid glycoprotein, feline, serum amyloid A, turbidimetric immunoassay.

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Evaluation of a Commercially Available Human Serum Amyloid A (SAA) Turbidimetric Immunoassay for Determination of Feline SAA Concentration

Cited by 53 publications

References 14 publications

Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours

Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours

The feline acute phase reaction

Verification of Measurement of the Feline Serum Amyloid A (SAA) Concentration by Human SAA Turbidimetric Immunoassay and Its Clinical Application

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