Evaluation of a Commercially Available Hyaluronic Acid Hydrogel (Restylane) as Injectable Scaffold for Dental Pulp Regeneration: An In Vitro Evaluation

Chrepa, Vanessa; Austah, Obadah; Diogenes, Aníbal

doi:10.1016/j.joen.2016.10.026

Cited by 51 publications

(38 citation statements)

References 42 publications

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“…These therapies explore the endogenous regenerative potential of the endodontic tissues and procure to enhance it by combining biopolymers with adequate biochemical stimuli [1]. Among the most reported biopolymers for endodontic therapy are hyaluronic acid (HA) [2][3][4], chitosan [5], alginate [6], gelatin [7][8][9], collagen [10,11], fibrin [12], and silk fibroin [13]. These natural-origin molecules resemble the extracellular matrix (ECM) components, providing unique properties to the specific regenerative approach, thereby enhancing their biocompatibility and biological recognition.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Almeida

Babo

Silva

et al. 2018

J Mater Sci: Mater Med

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The restoration of dentine-pulp complex remains a challenge for dentists; nonetheless, it has been poorly addressed. An ideal system should modulate the host response, as well as enable the recruitment, proliferation and differentiation of relevant progenitor cells. Herein was proposed a photocrosslinkable hydrogel system based on hyaluronic acid (HA) and platelet lysate (PL). PL is a cocktail of growth factors (GFs) and cytokines involved in wound healing orchestration, obtained by the cryogenic processing of platelet concentrates, and was expected to provide the HA hydrogels specific biochemical cues to enhance pulp cells' recruitment, proliferation and differentiation. Stable HA hydrogels incorporating PL (HAPL) were prepared after photocrosslinking of methacrylated HA (Met-HA) previously dissolved in PL, triggered by the Ultra Violet activated photoinitiator Irgacure 2959. Both the HAPL and plain HA hydrogels were shown to be able to recruit cells from a cell monolayer of human dental pulp stem cells (hDPSCs) isolated from permanent teeth. The hDPCs were also seeded directly over the hydrogels (5 × 10 cells/hydrogel) and cultured in osteogenic conditions. Cell metabolism and DNA quantification were higher, in all time-points, for PL supplemented hydrogels (p < 0,05). Alkaline phosphatase (ALPL) activity and calcium quantification peaks were observed for the HAPL group at 21 days (p < 0,05). The gene expression for ALPL and COLIA1 was up-regulated at 21 days to HAPL, compared with HA group (p < 0,05). Within the same time point, the gene expression for RUNX2 did not differ between the groups. Overall, data demonstrated that the HA hydrogels incorporating PL increased the cellular metabolism and stimulate the mineralized matrix deposition by hDPSCs, providing clear evidence of the potential of the proposed system for the repair of damaged pulp/dentin tissue and endodontics regeneration.

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Section: Introductionmentioning

confidence: 99%

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Almeida

Babo

Silva

et al. 2018

J Mater Sci: Mater Med

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“…HA displays unique structural and physiological functions within tissues, including extracellular and intracellular interactions, and growth factor modulation [51]. A recent study described a positive correlation between the HA scaffold and SCAP survival during REPs [52]. Moreover, HA-conveyed DAPs and TAPs appear more fluid and applicable inside the root canal.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Bactericidal Activity of a Hyaluronic Acid-Vehicled Clarithromycin Antibiotic Mixture by Confocal Laser Scanning Microscopy

et al. 2020

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Confocal laser scanning microscopy (CLSM) was used to evaluate the antibacterial effect and depth of action of a novel clarithromycin-containing triple antibiotic mixture, which was proposed for root canal disinfection in dental pulp regeneration. A previous study reported that this mixture had no tooth discoloration effects in vitro. After infection with Enterococcus faecalis for 3 weeks, the dentinal tubules in the cylindrical root specimens were exposed to different antibiotic mixtures: ciprofloxacin, metronidazole and minocycline (3-MIX); ciprofloxacin, metronidazole and clarithromycin (3-MIXC) and ciprofloxacin and metronidazole (2-MIX). Each antibiotic formulation was mixed with macrogol (MG) or hyaluronic acid (HA) vehicles. CLSM and viability staining were used to quantitatively analyze the mean depth of the antibacterial effect and the proportions of dead and live bacteria inside the dentinal tubules. The 3-MIX and 3-MIXC demonstrated a similar depth of action. The mean proportion of dead bacteria was similar in the 3-MIX and 3-MIXC groups, and both were statistically higher than that of 2-MIX (p = 0.014). Each antibiotic mixture showed a higher bactericidal efficacy if conveyed with HA, compared to MG (3-MIX, p = 0.019; 3-MIXC, p = 0.013 and 2-MIX, p = 0.0125). The depth of action and the antibacterial efficacy of 3-MIXC seemed comparable with 3-MIX.

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“…Thus, PLLA scaffold allowed us to successfully test the concept of MP tissue transplantation as a means to regenerate pulp-dentin complex. However, clinical translation of this approach will require use of injectable scaffold, such as peptide hydrogel (eg, PuraMatrix [3-D Matrix, Waltham, MA]) or hyaluronic acid-based polysaccharide molecules (eg, Restylane [Galderma, Lausanne, Switzerland]), due to incompatibility of PLLA for clinical application (23,24).…”

Section: Discussionmentioning

confidence: 99%

Minced Pulp as Source of Pulpal Mesenchymal Stem Cells with Odontogenic Differentiation Capacity

Liang

Kawano

Chen

et al. 2018

Journal of Endodontics

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Evaluation of a Commercially Available Hyaluronic Acid Hydrogel (Restylane) as Injectable Scaffold for Dental Pulp Regeneration: An In Vitro Evaluation

Cited by 51 publications

References 42 publications

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Evaluation of the Bactericidal Activity of a Hyaluronic Acid-Vehicled Clarithromycin Antibiotic Mixture by Confocal Laser Scanning Microscopy

Minced Pulp as Source of Pulpal Mesenchymal Stem Cells with Odontogenic Differentiation Capacity

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