Mol. Reprod. Dev.
 1996
DOI: 10.1002/(sici)1098-2795(199604)43:4<519::aid-mrd15>3.0.co;2-u
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Evaluating zona pellucida structure and function using antibodies to rabbit 55 kDa ZP protein expressed in baculovirus expression system

Sarvamangala V. Prasad
1
,
B Wilkins
2
,
Sheri M. Skinner
3
et al.
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Cited by 67 publications

(28 citation statements)
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“…Although different localizations of the zona proteins might reflect differences among mammalian species, we thought it possible that variations in the histochemical methods used to prepare the tissue before sectioning might be responsible for the divergent results. Thus, when Bouin's fluid was used as the fixative, zona protein staining was detected between the granulosa cells surrounding the zona pellucida matrix as well as in the zona pellucida matrix in porcine (Sinowatz et al 1995) and rabbit ovaries (Prasad et al 1996). Accordingly, we saw the same staining pattern between granulosa cells as the two aforementioned groups when we used this fixation technique (see Fig.…”
Section: Discussionsupporting
confidence: 87%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…Although different localizations of the zona proteins might reflect differences among mammalian species, we thought it possible that variations in the histochemical methods used to prepare the tissue before sectioning might be responsible for the divergent results. Thus, when Bouin's fluid was used as the fixative, zona protein staining was detected between the granulosa cells surrounding the zona pellucida matrix as well as in the zona pellucida matrix in porcine (Sinowatz et al 1995) and rabbit ovaries (Prasad et al 1996). Accordingly, we saw the same staining pattern between granulosa cells as the two aforementioned groups when we used this fixation technique (see Fig.…”
Section: Discussionsupporting
confidence: 87%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…In mouse, ZPB has been assigned only a structural role (Greve and Wassarman, 1985). However, in rabbit, porcine and bonnet monkey, ZPB has been shown to bind to homologous spermatozoa (Prasad et al, 1996;Yurewicz et al, 1998;Govind et al, 2001). The results of this study show that ZPB may also have a role to play in acrosomal exocytosis in humans.…”
Section: Discussionmentioning
confidence: 70%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…We have opted for the baculovirus expression system over the mammalian expression system as recombinant human ZP1, ZP3 and ZP4 obtained using this expression system not only bind to capacitated acrosome-intact spermatozoa but also induce acrosome reaction in a dose dependent manner [9,11,12,16,32]. These studies are further corroborated by observations that baculovirus-expressed recombinant rabbit 55 kDa protein (homolog of human ZP4) binds in a dose dependent manner to the rabbit sperm [33]. However, recombinant porcine ZP4 expressed in insect cells binds to bovine but not porcine sperm which may be due to the glycosylation profile of recombinant protein being similar to bovine rather than porcine ZP4 [34].…”
Section: Discussionmentioning
confidence: 94%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
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