Evaluating two multistrain probiotics on growth performance, intestinal morphology, lipid oxidation and ileal microflora in chickens

Abstract

An experiment was conducted to investigate the supplementation of two commercially available multistrain probiotics as an alternative to antibiotics on growth performance, intestinal morphology, lipid oxidation and ileal microflora in broiler chickens. A total of 280‐day‐old ROSS 308 mixed‐sex broiler chickens with an average initial body weight of 42 ± 0.5 g were randomly divided into four treatments with five replicate cages of 14 birds each cage in a completely randomized design and fed with the following diets for 42 day: (a) control (CON) (antibiotic‐free diet), (b) antibiotic (ANT) (CON + Avilamycin 150 g/ton feed), (c) probiotic A (CON + Protexin® 150 g/ton feed) and (d) probiotic B (CON + Bio‐Poul® 200 g/ton feed). The results showed the broilers fed the ANT diet had greater average daily gain than broilers fed the CON diet during day 1–14 (p < 0.05). At day 42, two birds were randomly selected per replicate for evaluation intestinal morphology, lipid oxidation and ileal microflora. birds fed diet supplemented with probiotic A and probiotic B increased villus height and goblet cells numbers in the jejunum and villus height to crypt depth ratio and villus height in the ileum as compared to birds fed CON diet (p < 0.05). The malondialdehyde value was reduced (p < 0.05) in the ANT, probiotic B and probiotic A groups compared with the CON group. The Lactobacillus population was increased and Clostridium spp. population decreased in the ileum of broilers fed diets containing the probiotic B and probiotic A compared with those fed CON diet (p < 0.05). The results from this study indicate that the probiotic A (Protexin®) and probiotic B (Bio‐Poul®) used in this trial may serve as alternatives to ANT.

Effects of in ovo feeding of N-acetyl-L-glutamate on early intestinal development and growth performance in broiler chickens

“…Nutritional modulation-induced improvement of the growth performance is often accompanied by the improvement of intestinal function in broilers ( Boroojeni et al., 2019 , Kazemi et al., 2019 ). However, whether nutrition regulation first improves intestinal function and then affects the growth performance of broilers remains to be elucidated.…”

Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review

“…Each probiotic strain confers varying levels of protective efficacy, which is why many commercial products use multi-strain probiotics. Multiple-strain and multi-species probiotics act on different sites and provide different modes of action that create synergistic effects [ 22 , 23 , 24 ]. The genera of probiotic microorganisms commonly used for poultry include Bifidobacterium , Lactococcus , Lactobacillus , Bacillus , Streptococcus, and yeast such as Candida .…”

Multi-Strain Probiotics: Synergy among Isolates Enhances Biological Activities

“…Likewise, multi-strain probiotics containing L. acidophilus , B. subtilis DSM 17299, and Clostridium butyricum increases the serum level of IgA and IgM with a decline in the count of E. coli in the feces of broiler chickens [ 86 ]. Furthermore, supplementing poultry feed with multi-strain probiotics was reported to result in a rise in villus length and the number of goblet cells in the jejunum, and villus height to crypt ratio in the ileum of chickens [ 132 ]. Feeding of chickens with citrus-Junos by-product fermented with multi-strain probiotics (containing S. cerevisiae , E. faecium , L. acidophilus and B. subtilis ) also increases the weight gain and mean daily feed consumption [ 111 ].…”