Evaluating the use of optical coherence tomography for the detection of epithelial cancers in vitro

Abstract

Abstract. Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial structure could greatly improve early diagnosis and long-term monitoring in patients. Here, we have used tissue-engineered (TE) models of normal skin and oral mucosa to generate models of melanoma and oral cancer. We have used these to determine the ability of OCT to image epithelial differences in vitro. We report that while in vivo OCT gives reasonable depth information for both skin and oral mucosa, in vitro the information provided is less detailed but still useful. OCT can provide reassurance on the development of TE models of skin and oral mucosa as they develop in vitro. OCT was able to detect the gross alteration in the epithelium of skin and mucosal models generated with malignant cell lines but was less able to detect alteration in the epithelium of TE models that mimicked oral dysplasia or, in models where tumor cells had penetrated into the dermis. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

Polymersome-Mediated Delivery of Combination Anticancer Therapy to Head and Neck Cancer Cells: 2D and 3D in Vitro Evaluation

“…A rhodamine 6G-based initiator prepared in-house (83.8 mg, 0.135 mmol) was dissolved in methanol (0.75 ml) and added to the MPC. The solution was deoxygenated by bubbling N2 for 30 10 mmol) and methanol (2 ml) was injected into the flask and 48 h later the reaction solution was diluted with methanol (~70 ml) and opened to the atmosphere. When the suspension turned green, 200 ml chloroform was added to dissolve the copolymer and the solution passed through a silica column to remove the catalyst.…”

Modern non-invasive diagnostic techniques in the detection of early cutaneous melanoma

“…OCT was used for monitoring non-surgical treatment (imiquimod) of lentigo maligna. [43][44][45] The results of these studies showed a good correlation between OCT and pathology. In OCT melanoma is characterized by marked architectural disarray and presence of vertical, icicle-shaped structures.…”

Marine Collagen as A Promising Biomaterial for Biomedical Applications

“…There is a similar need to provide an oral mucosa substitute, suitable for reconstructing primary or secondary oral mucosal defects due to limited viable donor-derived oral mucosal soft tissues. An ex vivo-produced oral mucosa equivalent has been successfully constructed and used for reconstruction of oral mucosal defects derived from oncologic resection, vestibuloplasty, and periodontal therapy [149,150,151]. An MC/chitosan composite scaffold was fabricated using isolated tilapia scale collagen via the process of lyophilization and dehydrothermal cross-linking at 130 °C in vacuo for use as a tissue engineered oral mucosa equivalent [152].…”