Evaluating the tumor stratification with a lysosomal pH sensitive-probe by fluorescence lifetime imaging

Yan, Jun; Lü, Xing; Zhang, Qian; Wang, Luolin; Lin, Weiying

doi:10.1016/j.cclet.2023.108408

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…In addition, their pH responsiveness was assessed at different pH values. It was worth noting that the pH was approximately 4.5 within lysosomes, , we hoped our compounds primarily colocalized in lysosomes. For instance, the broad absorption band of compound 10f at 480 nm (pH 4.5) exhibited a slight hypo-chromatic shift to 475 nm (pH 7.4).…”

Section: Resultsmentioning

confidence: 99%

Development of Novel β-Carboline/Furylmalononitrile Hybrids as Type I/II Photosensitizers with Chemo-Photodynamic Therapy and Minimal Toxicity

Xie,

Sun,

Pan

et al. 2024

Mol. Pharmaceutics

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Chemo-photodynamic therapy is a treatment method that combines chemotherapy and photodynamic therapy and has demonstrated significant potential in cancer treatment. However, the development of chemo-photodynamic therapeutic agents with fewer side effects still poses a challenge. Herein, we designed and synthesized a novel series of β-carboline/furylmalononitrile hybrids 10a–i and evaluated their chemo-photodynamic therapeutic effects. Most of the compounds were photodynamically active and exhibited cytotoxic effects in four cancer cells. In particular, 10f possessed type-I/II photodynamic characteristics, and its 1O2 quantum yield increased by 3-fold from pH 7.4 to 4.5. Most interestingly, 10f exhibited robust antiproliferative effects by tumor-selective cytotoxicities and hypoxic-overcoming phototoxicities. In addition, 10f generated intracellular ROS and induced hepatocellular apoptosis, mitochondrial damage, and autophagy. Finally, 10f demonstrated extremely low acute toxicity (LD50 = 1415 mg/kg) and a high tumor-inhibitory rate of 80.5% through chemo-photodynamic dual therapy. Our findings may provide a promising framework for the design of new photosensitizers for chemo-photodynamic therapy.

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Section: Resultsmentioning

confidence: 99%

Development of Novel β-Carboline/Furylmalononitrile Hybrids as Type I/II Photosensitizers with Chemo-Photodynamic Therapy and Minimal Toxicity

Xie,

Sun,

Pan

et al. 2024

Mol. Pharmaceutics

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“…Fluorescence is easily quenched due to the deprotonation effect, resulting in the reduction of fluorescence lifetime. 4,37,38 With a gradual increase in pH (pH = 5−8), the TPF imaging and TP-FLIM imaging do not change significantly (Figure 5b), suggesting that the imaging of FX2-MnCl 2 in cellular environments has a stable electronic structure and is not affected by the deprotonation effect at different pH conditions. This phenomenon proves the potential of manganese complexes In Vivo Imaging of FX2-MnCl 2 with ATP.…”

mentioning

confidence: 99%

Three–Four Photon Transition Mn(II) Complex Monitoring Lysosome-Related ATP in Real Time via Fluorescence Lifetime Imaging

Li,

Zhang,

Wang

et al. 2024

Anal. Chem.

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Currently, in situ monitoring of the adenosine triphosphate (ATP) level in lysosomes is critical to understand their involvement in various biological processes, but it remains difficult due to the interferences of limited targeting and low resolution of fluorescent probes. Herein, we report a classic Mn(II) probe (FX2-MnCl 2 ) with near-infrared (NIR) nonlinear (NLO) properties, accompanied by three−four photon transition and fivefold fluorescence enhancement in the presence of ATP. FX2-MnCl 2 combines with ATP through dual recognition sites of diethoxy and manganese ions to reflect slightly fluorescence lifetime change. Through the synergy of multiphoton fluorescence imaging (MP-FI) and multiphoton fluorescence lifetime imaging microscopy (MP-FLIM), it is further demonstrated that FX2-MnCl 2 displays lysosome-specific targeting behavior, which can monitor lysosome-related ATP migration under NIR laser light. This work provides a novel multiphoton transformation fluorescence complex, which might be a potential candidate as a simple and straightforward biomarker of lysosome ATP in vitro for clinical diagnosis.

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Recent advances in molecular and nanoparticle probes for fluorescent bioanalysis

Luo,

Tian,

Zhang

et al. 2024

Nano Res.

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Evaluating the tumor stratification with a lysosomal pH sensitive-probe by fluorescence lifetime imaging

Cited by 5 publications

References 39 publications

Development of Novel β-Carboline/Furylmalononitrile Hybrids as Type I/II Photosensitizers with Chemo-Photodynamic Therapy and Minimal Toxicity

Development of Novel β-Carboline/Furylmalononitrile Hybrids as Type I/II Photosensitizers with Chemo-Photodynamic Therapy and Minimal Toxicity

Three–Four Photon Transition Mn(II) Complex Monitoring Lysosome-Related ATP in Real Time via Fluorescence Lifetime Imaging

Recent advances in molecular and nanoparticle probes for fluorescent bioanalysis

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