DOI: 10.7213/estud.biol.35.085.ao01
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Abstract: The purpose of this study was to assess the specificity of distinct primers used in the molecular diagnosis for Leishmania detection by using biological sequence search and alignment tools. Four primer pairs routinely employed in the PCR-based diagnostics for Leishmaniasis detection were evaluated through the software Primer-BLAST, which compares nucleotide sequences against user-selected database to avoid primer pairs cause non-specific amplifications. The LU5A-LB3C primer pair showed good specificity among a… Show more

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