Evaluating the effect of two-dimensional molecular layout on DNA origami-based transporters

Fukumoto, Kodai; Miyazono, Yuya; Ueda, Takuya; Harada, Yoshie; Tadakuma, Hisashi

doi:10.1039/d3na00088e

Cited by 2 publications

(1 citation statement)

References 51 publications

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“…DNA nanotechnology offers nanometer-sized, well-ordered precise structures. − This technology has been used for precise alignment of functional binders, such as aptamers, antibodies, chemical compounds, and nanoparticles for specific capture of target molecules. − Particularly, 3D DNA origami has extensionally provided user-defined features and enabled specific virus capture or cell recognition. − However, the application of this technology is limited for EV capture, especially as past approaches require a large number of nanostructures to capture an EV, limiting size discrimination capability. − Here, we demonstrate a novel method for selective capture of EVs that have a user-defined vesicle size and surface protein marker. Using a geometrical structural feature of 3D DNA origami, we captured EVs of a specific size from samples containing a broad distribution of vesicles.…”

Section: Introductionmentioning

confidence: 99%

Size-Selective Capturing of Exosomes Using DNA Tripods

Iinuma,

Chen,

Masubuchi

et al. 2024

J. Am. Chem. Soc.

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Fractionating and characterizing target samples are fundamental to the analysis of biomolecules. Extracellular vesicles (EVs), containing information regarding the cellular birthplace, are promising targets for biology and medicine. However, the requirement for multiple-step purification in conventional methods hinders analysis of small samples. Here, we apply a DNA origami tripod with a defined aperture of binders (e.g., antibodies against EV biomarkers), which allows us to capture the target molecule. Using exosomes as a model, we show that our tripod nanodevice can capture a specific size range of EVs with cognate biomarkers from a broad distribution of crude EV mixtures. We further demonstrate that the size of captured EVs can be controlled by changing the aperture of the tripods. This simultaneous selection with the size and biomarker approach should simplify the EV purification process and contribute to the precise analysis of target biomolecules from small samples.

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