Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction

Abstract

Bacterial adhesion potential constitutes the transition of bacteria from the planktonic to the static phase by promoting biofilm formation, which plays a significant role in plant-microbial interaction in the agriculture industry. In present study, the adhesion potential of five soil-borne bacterial strains belonging to different genera was studied. All bacterial strains were capable of forming colonies and biofilms of different levels of firmness on polystyrene. Significant variation was observed in hydrophobicity and motility assays. Among the five bacterial strains (SH-6, SH-8, SH-9, SH-10, and SH-19), SH-19 had a strong hydrophobic force, while SH-10 showed the most hydrophilic property. SH-6 showed great variability in motility; SH-8 had a swimming diffusion diameter of 70 mm, which was three times higher than that of SH-19. In the motility assay, SH-9 and SH-10 showed diffusion diameters of approximately 22 mm and 55 mm, respectively. Furthermore, among the five strains, four are predominately electron donors and one is electron acceptors. Overall, positive correlation was observed among Lewis acid base properties, hydrophobicity, and biofilm forming ability. However, no correlation of motility with bacterial adhesion could be found in present experimental work. Scanning electron microscopy images confirmed the adhesion potential and biofilm ability within extra polymeric substances. Research on the role of adhesion in biofilm formation of bacteria isolated from plants is potentially conducive for developing strategies such as plant–microbial interaction to mitigate the abiotic stress.

Seed Bio-priming of wheat with a novel bacterial strain to modulate drought stress in Daegu, South Korea

“…Scanning electron microscopy (SEM) revealed the presence of a bacterial biofilm and allowed observation of its surface properties. Our team recently reported the biochemical, adhesive, and biofilm characteristics of various novel bacterial strains, including SH-8 ( Shaffique et al., 2022a ). Here, we report the results from a preliminary study, including the results of SEM.…”

“…The procedure of scanning electron microscopy (SEM) followed was that described by Shaffique et al. ( Shaffique et al., 2022a ).…”

Specific Streptomyces strain enhances the growth, defensive mechanism, and fruit quality of cucumber by minimizing its fertilizer consumption

“…The direct method includes biofertilization, root growth stimulation, rhizo-remediation, and plant stress control, whereas the indirect method includes induction of systemic resistance, competition for nutrients, and biological control to reduce disease levels [ 17 , 18 ]. Streptomyces is a Gram-positive bacteria with an exceptional ability to survive in extreme environments such as salt and drought stress [ 19 – 21 ]. These microorganisms exhibit plant growth promotion combined with biocontrol properties [ 22 – 25 ].…”

Screening of the Ability of Microorganisms to Biofilm Formation

“…Biofilms are a barrier to protect microorganisms from adverse conditions such as degradation, desiccation, UV radiation, and exposure to chemical compounds [1,2]. They can help retain and stabilize microorganisms in appropriate environments, providing optimal conditions for their existence and functioning and adhesion to various surfaces [3,4,5], which can be important for the formation of biological communities and biogeochemical cycles. These structures can interact with the environment, including the exchange of substances with other microorganisms, the formation of covalent or non-specific bonds with chemical compounds [6,7,8].…”