Evaluating effect of alginate/chitosan hydrogel containing 4-Methylcatechol on peripheral nerve regeneration in rat model

Abbaszadeh-Goudarzi, Ghasem; Haghi-Daredeh, Saeed; Ehterami, Arian; Rahmati, Majid; Nazarnezhad, Simin; Hashemi, Seyedeh Fatemeh; Niyakan, Maryam; Vaez, Ahmad; Salehi, Majid

doi:10.1080/00914037.2020.1785462

Cited by 14 publications

(7 citation statements)

References 52 publications

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“…44 Sciatic nerve regeneration was assessed by Walking-foot-print analysis, HPL, and gastrocnemius muscle wet weight loss. 45,46 The highest 47 The HPL was analyzed and the finest result was 4.66 ± 1.15 s belonged to the Chit-TAG/ Tau1% while other research assessed the result of Hesperidin on sciatic nerve regeneration announced HPL of 5.33 ± 0.57 s. 12 Our conclusions have significant implications in consuming Chit-TAG/Tau hydrogel for healing PNI, but still more evaluations are needed to check this healing approach.…”

Section: Discussionmentioning

confidence: 80%

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Peripheral nerve regeneration by thiolated chitosan hydrogel containing Taurine: In vitro and in vivo study

Ehtermi

Kolarijani

Nazarnezhad

et al. 2022

Journal of Bioactive and Compatible Polymers

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About 2.8% of trauma sick persons hurt from peripheral nerve damages, thus, numerous approaches are using to improve peripheral nerve regeneration. In the current study, the efficacy of several dosages of Taurine for peripheral nerve regeneration was evaluated. About 0.1%, 1%, and 10% (w/w) of Taurine were added into thiolated chitosan hydrogel and its features including morphology, swelling properties, weight loss, hemo-, and cytocompatibility were assessed. Hydrogels’ functionality was evaluated by injecting them into the crushed sciatic nerve of rats by using walking-foot-print analysis, Hot plate latency test, gastrocnemius muscle wet weight loss, and histopathological evaluation. Results demonstrated that the average pore size is in the area of 30–40 μm with interconnected pores and their weight loss was around 70% after 7 days. Results of blood compatibility and the MTT tests confirmed the biocompatibility of hydrogels. In vivo study illustrate thiolated Chitosan/Taurine hydrogels especially hydrogel includes 1% of Taurine enhanced sciatic nerve regeneration. In conclusion, Taurine can be used as a feasible treatment for peripheral nerve regeneration.

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Section: Discussionmentioning

confidence: 80%

“…Abbaszadeh-Goudarzi et al improving sciatic nerve regeneration by adding 4-Methylcatechol into Alginate/Chitosan hydrogel and they expressed SFI of −42.28 ± 4.59 after 4 weeks and −22.13 ± 6.28 after 8 weeks for their best group had 10% 4-Methylcatechol. 47…”

Section: Discussionmentioning

confidence: 99%

Peripheral nerve regeneration by thiolated chitosan hydrogel containing Taurine: In vitro and in vivo study

Ehtermi

Kolarijani

Nazarnezhad

et al. 2022

Journal of Bioactive and Compatible Polymers

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“…The results show that the addition of PEtOx reduces the rate of weight loss and degradability. Moreover, the GelMA/PEtOx hydrogel is able to support its role as a tissue engineering material for nerve regeneration in a short time 56 …”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the GelMA/PEtOx hydrogel is able to support its role as a tissue engineering material for nerve regeneration in a short time. 56…”

Section: Weight Loss Studiesmentioning

confidence: 99%

Sciatic nerve injury regeneration in adult male rats using gelatin methacrylate (GelMA)/poly(2‐ethy‐2‐oxazoline) (PEtOx) hydrogel containing 4‐aminopyridine (4‐AP)

Mahand

Jahanmardi

Kruppke

et al. 2023

J Biomedical Materials Res

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One of the most important parts of the body is the peripheral nervous system, and any injuries in this system may result in potentially lethal consequences or severe side effects. The peripheral nervous system may not rehabilitate the harmed regions following disabling disorders, which reduce the quality of life of patients. Fortunately, in recent years, hydrogels have been proposed as exogenous alternatives to bridge damaged nerve stumps to create a useful microenvironment for advancing nerve recovery. However, hydrogel-based medicine in the therapy of peripheral nerve injury still needs a lot of improvement. In this study, GelMA/PEtOx hydrogel was used for the first time to deliver 4-Aminopyridine (4-AP) small molecules. 4-AP is a broad-spectrum potassium channel blocker, which has been demonstrated to increase neuromuscular function in patients with various demyelinating disorders.The prepared hydrogel showed a porosity of 92.2 ± 2.6% after 20 min, swelling ratio of 456.01 ± 2.0% after 180 min, weight loss of 81.7 ± 3.1% after 2 weeks, and good blood compatibility as well as sustainable drug release. MTT analysis was performed to assess the cell viability of the hydrogel and proved that the hydrogel is an appropriate substrate for the survival of cells. In vivo studies were performed for functional analysis and the sciatic functional index (SFI) as well as hot plate latency results showed that the use of GelMA/PEtOx+4-AP hydrogel enhances the regeneration compared to the GelMA/PEtOx hydrogel and the control group.

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“…4-MC loaded into the hydrogels showed no cytotoxic effects and improved proliferation of PC12 cells. The results of functional and histopathological tests demonstrated that 10% of 4-MC loaded into the hydrogel significantly enhanced sciatic nerve regeneration after 8 weeks compared to the negative group [ 162 ], thus concluding that fabricated hydrogel can also be used as a suitable delivery material not only for stem cells but also for other compounds.…”

Section: Cardiac and Nervous Tissuesmentioning

confidence: 99%

Chitosan-Based Biomaterials for Tissue Regeneration

et al. 2023

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Chitosan is a chitin-derived biopolymer that has shown great potential for tissue regeneration and controlled drug delivery. It has numerous qualities that make it attractive for biomedical applications such as biocompatibility, low toxicity, broad-spectrum antimicrobial activity, and many others. Importantly, chitosan can be fabricated into a variety of structures including nanoparticles, scaffolds, hydrogels, and membranes, which can be tailored to deliver a desirable outcome. Composite chitosan-based biomaterials have been demonstrated to stimulate in vivo regeneration and the repair of various tissues and organs, including but not limited to, bone, cartilage, dental, skin, nerve, cardiac, and other tissues. Specifically, de novo tissue formation, resident stem cell differentiation, and extracellular matrix reconstruction were observed in multiple preclinical models of different tissue injuries upon treatment with chitosan-based formulations. Moreover, chitosan structures have been proven to be efficient carriers for medications, genes, and bioactive compounds since they can maintain the sustained release of these therapeutics. In this review, we discuss the most recently published applications of chitosan-based biomaterials for different tissue and organ regeneration as well as the delivery of various therapeutics.

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Evaluating effect of alginate/chitosan hydrogel containing 4-Methylcatechol on peripheral nerve regeneration in rat model

Cited by 14 publications

References 52 publications

Peripheral nerve regeneration by thiolated chitosan hydrogel containing Taurine: In vitro and in vivo study

Peripheral nerve regeneration by thiolated chitosan hydrogel containing Taurine: In vitro and in vivo study

Sciatic nerve injury regeneration in adult male rats using gelatin methacrylate (GelMA)/poly(2‐ethy‐2‐oxazoline) (PEtOx) hydrogel containing 4‐aminopyridine (4‐AP)

Chitosan-Based Biomaterials for Tissue Regeneration

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