Euschistus heros mass rearing technique for the multiplication of Telenomus podisi

Abstract

-The objective of this work was to improve the mass rearing technique of Euschistus heros in laboratory. Nymphs and adults were reared at densities 100, 200, 300 and 400 eggs per Petri dish (9 cm diameter), and at 50, 100, 150 and 200 couples per rearing cages (900 mL), respectively. Survival rate of immature stages and survivorship and reproduction of adults were determinated. Survivorship of nymph to adult was the highest (89%) at density 100 eggs per dish. Adult survivorship was independent of density, and 100 couples per cage were the best to improve quality of the produced progeny. In these conditions, fecundity was 160.8±9.28 eggs per female, and a total of 8,950±456 eggs per cage per month was produced. Two hundred couples per cage showed a negative effect on reproduction, which decreased to 65%. With this technique, a colony of 35 cages with 100 couples per cage yields about 313.3 thousands eggs per month, which is enough to supply the egg parasitoid Telenomus podisi to colonize about 35 ha of soybean field.Index terms: biological control, density, egg parasitoid, neotropical brown stink bug. Técnica de criação massal de Euschistus heros para a multiplicação de Telenomus podisiResumo -O objetivo deste trabalho foi melhorar a técnica de criação massal de Euschistus heros em laboratório. Ninfas e adultos foram criados nas densidades de 100, 200, 300 e 400 ovos por placa de Petri (9 cm diâmetro), e 50, 100, 150 e 200 casais por gaiola de criação (900 mL), respectivamente. Foram determinadas a taxa de sobrevivência dos estágios imaturos e a sobrevivência e reprodução de adultos. A sobrevivência das ninfas até o estágio de adulto foi maior (89%) quando criadas em densidade de 100 ovos por placa de Petri. A sobrevivência dos adultos foi independente da densidade, e 100 casais por gaiola de criação foi a melhor densidade encontrada para melhorar a qualidade dos insetos. Nessas condições, a fecundidade das fêmeas foi de 160,8±9,28 ovos por fêmea, e um total de 8.950±456 ovos por gaiola por mês foi produzido. A densidade de 200 casais por gaiola apresentou efeito negativo sobre a reprodução, que decresceu 65%. Com esta técnica, uma colônia de 35 gaiolas com 100 casais por gaiola produz cerca de 313,3 mil ovos por mês, quantidade suficiente para liberar o parasitóide de ovos Telenomus podisi em 35 ha de plantio de soja.Termos para indexação: controle biológico, densidade, parasitóide de ovos, percevejo marrom.

Survivorship and Egg Production of Phytophagous Pentatomids in Laboratory Rearing

“…Chocorosqui & Panizzi (2008) (Fig 1). These results demonstrate that E. heros can keep their egg production high over a period of 30 days, as also observed by Peres & Corrêa-Ferreira (2001) and Silva et al (2008). On the other hand, N. viridula and D. melacanthus decreased their fecundity during the last 10-Means (n = 3) followed by the same letter in each column do not differ signi icantly using the Tukey test (P > 0.05); ns non signi icant.…”

“…Furthermore, biological control of these bugs using egg parasitoids is an important issue that demands laboratory colonies (Peres & Corrêa-Ferreira 2004, Silva et al 2008.…”

“…The relative success in rearing N. viridula is achieved using a combination of whole soybean plants, dry soybean seeds and raw shelled peanuts (Corrêa-Ferreira 1985). Euschistus heros is more easily reared in the laboratory using green bean pods, dry soybean, peanuts and sun lower seeds (Silva et al 2008), while D. melacanthus can be maintained on dry soybean seeds and corn seedlings (R. Bianco, personal communication).…”

Selectivity of pesticides used in rice crop on Telenomus podisi and Trichogramma pretiosum

“…Eggs of alternate hosts Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) (Parra 1997) and Euschistos heros (Fabricius) (Hemiptera: Pentatomidae) (Borges et al 2006) and adults of T. pretiosum (Hassan et al 2000) and T. podisi (Perez & Corrêa-Ferreira 2004, Silva et al 2008 were obtained from mass rearing in the laboratory (temperature: 25 ± 1 ºC; relative humidity: 70 ± 10 %; photophase: 14 h).…”

Biological Parameters of Euschistus heros (F.) (Heteroptera: Pentatomidae) and its Susceptibility to Entomopathogenic Fungi When Fed on Different Diets

“…Since then, new stink bugs eggs collected in the field have been added annually. The insects were maintained in controlled environment rooms (26 ± 2ºC, 60 ± 10% RH and a 14-hour photoperiod) according to a breeding method modified from Silva et al (2008). After hatching, the nymphs were stored in covered crystal-polyester 11 x 11 x 3 cm boxes until they reached the 4 th instar.…”