Ethylene treatment induces changes in folate profiles in climacteric fruit during postharvest ripening

García‐Salinas, Carolina; Ramos-Parra, Perla A.; Garza, Rocío I. Díaz de la

doi:10.1016/j.postharvbio.2016.03.011

Cited by 24 publications

(6 citation statements)

References 29 publications

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“…Recombinant plant GGHs have been produced in engineered E. coli [40,41], which enabled faster and more accurate folate analysis in papaya, tomato, banana and avocado fruit by the use of HPLC coupled to a four-channel electrochemical detector [42]. Progress in mass spectrometry resulted in worldwide use of various MS/MS detectors, which makes them suitable for development of a chromatographic standard method.…”

Section: A C C E P T E D Mmentioning

confidence: 99%

The use of a plant enzyme for rapid and sensitive analysis of naturally-occurring folates in food by liquid chromatography-tandem mass spectrometry

Švarc

García‐Salinas

Garza

et al. 2019

Journal of Chromatography A

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Section: A C C E P T E D Mmentioning

confidence: 99%

The use of a plant enzyme for rapid and sensitive analysis of naturally-occurring folates in food by liquid chromatography-tandem mass spectrometry

Švarc

García‐Salinas

Garza

et al. 2019

Journal of Chromatography A

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“…Folic Acid analyses of ripe banana pulp were conducted at PT Saraswanti Indo Genetech Laboratory in Duplo reactions for each sample and repeated three times. Three fingers randomly selected from the middle hands, then washed, peeled, cut into 0.5-0.7 cm thick longitudinal wedges, flesh-frozen in liquid nitrogen, and kept at -80°C for folic acid extraction (Garcia-Salinas et al 2016). Folic acid is extracted and purified refers to a method Rahimi and Goodarzi (2011) that has been modified.…”

Section: Folic Acid Analysismentioning

confidence: 99%

Folic acid content and fruit characteristics of five Indonesian dessert banana cultivars

Ningsih

Megia

2018

Biodiversitas

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Ningsih R, Megia R. 2019. Folic acid content and fruit characteristics of five Indonesian dessert banana cultivars. Biodiversitas 20: 144-151. Folic acid plays an important role in the many metabolic functions of organism, especially pregnant women. Whereas banana is a fruit that rich in nutrients, high productivity, high diversity, well known, and widely consumed in many countries and by various communities from infants to elderly. The research aimed to analyze folic acid content and describe the morphology of mature fruits at three different genomic groups of five Indonesian dessert banana cultivars namely Ambon Kuning (AAA), Ambon Lumut (AAA), Raja Sereh (AAB), Raja Bulu (AAB), and Lampung (AA). The folic acid content of fruit was analyzed using HPLC equipped with a UV-vis detector. Fruit characters were analyzed based on 21 descriptors then subjected to clustering analysis. The result shows that folic acid content of mature stage was 22.26; 21.39; 22.59; 21.2; and 24.58 μg/100 g fresh weight for those five banana cultivars respectively. Lampung cultivar showed the highest value. Analysis of variance followed by DMRT at 5% probability showed that folic acid, bunch weight, fruit weight, and fruit length were significantly diverse among those five cultivars. Clustering analyses revealed that two groups were formed. The first comprises of Ambon Kuning and Ambon Lumut while the second of Raja Sereh, Lampung, and Raja Bulu. Lampung cultivar was closely related to Raja Sereh than the other cultivars. Adjusted Rand Index analyzed concluded that the immature peel color, mature pulp color, and predominant taste were the best distinctive characters. This knowledge can be used as a consideration for the plant breeding program especially in nutrition improvement.

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“…During oriental melon ripening, components of the quality index, such as sugar, colour, texture, flavour and volatiles etc., change significantly [ 2 ]. At the same time, a large number of proteins and genes expression changed concomitant with ripening [ 3 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

Guo

Cui

et al. 2017

BMC Plant Biol

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BackgroundOriental melon is one of the most popular crops for its nutritional and flavour quality. Components that determine melon quality, such as sugar, colour, texture, flavour and aroma, among other factors, accumulate in different developmental stages. Thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in the oriental melon is very important for advancing our understanding of oriental melon quality in the ripening processes.ResultsiTRAQ-based protein profiling was conducted on ‘YuMeiren’ oriental melon fruit at different developmental stages. Physiological quality indices, including firmness, rind colour, soluble solids content (SSC), ethylene production, sugar content and volatile compounds were also characterized during four maturity periods of the melon, including 5, 15, 25 and 35 days after anthesis (DAA). A principal component analysis (PCA) revealed that the aroma volatiles at 5 DAA and 15 DAA were similar and separated from that of 35 DAA. More than 5835 proteins were identified and quantified in the two biological repeats and divided into 4 clusters by hierarchical cluster analysis. A functional analysis was performed using Blast2GO software based on the enrichment of a GO analysis for biological process, molecular function and cellular components. The main KEGG pathways, such as glycolysis, α-linolenic acid and starch and sucrose metabolism, were analyzed.The gene family members corresponding to differentially expressed proteins, including lipoxygenase (CmLOX01-18) and alcohol acetyltransferase (CmAAT1-4) involved in the α-linolenic acid metabolic pathway, were verified with real-time qPCR. The results showed that the expression patterns of 64.7% of the genes were consistent with the expression patterns of the corresponding proteins.ConclusionsThis study combined the variation of the quality index and differentially expressed proteins of oriental melon at different developmental stages that laid the foundation for the subsequent protein and gene function validation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-0977-7) contains supplementary material, which is available to authorized users.

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Ethylene treatment induces changes in folate profiles in climacteric fruit during postharvest ripening

Cited by 24 publications

References 29 publications

The use of a plant enzyme for rapid and sensitive analysis of naturally-occurring folates in food by liquid chromatography-tandem mass spectrometry

The use of a plant enzyme for rapid and sensitive analysis of naturally-occurring folates in food by liquid chromatography-tandem mass spectrometry

Folic acid content and fruit characteristics of five Indonesian dessert banana cultivars

iTRAQ-based Protein Profiling and Fruit Quality Changes at Different Development Stages of Oriental Melon

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