Ethylene regulates ascorbic acid content during dark-induced leaf senescence

Gergoff, Gustavo; Chaves, Alicia Raquel; Bártoli, Carlos Guillermo

doi:10.1016/j.plantsci.2009.12.003

Cited by 43 publications

(24 citation statements)

References 27 publications

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“…The higher levels of glutathione and AsA observed in SA-deficient plants may contribute to their alleviated symptoms (Wang et al, 2011a). In contrast, other studies have shown that these signaling molecules have no effect on AsA content (Keramat et al, 2009;Gergoff et al, 2010;Krajnc et al, 2011), suggesting that the effects of signaling molecules on AsA accumulation differ between species. The apple variety 'Prima' has a higher than expected L-AsA content at harvest, which is associated with improved resistance to necrotrophic pathogens.…”

Section: The Roles Of Asa During Pathogenesismentioning

confidence: 87%

Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

Hou

Li²,

Gao³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Using rapid amplification of cDNA ends, a full-length cDNA sequence of a GDP-L-galactose phosphorylase-like gene was isolated from leaves infected by Erysiphe necator in the Chinese wild (Vitis pseudoreticulata) clone, 'Baihe-35-1', an E. necator-resistant genotype. The full-length cDNA, designated as VpVTC, comprised 1943 bp and putatively encodes a 453-amino acid polypeptide containing an HIT motif. The deduced amino acid sequence showed high similarity with that of VTC genes from other plants. The expression of VpVTC, determined by reverse transcriptase-polymerase chain reaction, was induced by E. necator and defense signaling molecules, including salicylic acid, methyl jasmonate, and ethephon, in 'Baihe-35-1', the V. quinquangularis genotype 'Shang-24', and the E. necator-susceptible A GGP-like gene from a Chinese wild Vitis species V. pseudoreticulata genotype, 'Hunan-1'. Transcript levels of VpVTC correlated well with the degree of disease resistance in the 3 genotypes. Maximum induction of VpVTC by E. necator (>7-fold at 96 h postinoculation) occurred in 'Baihe-35-1', which also showed the fastest response to signaling molecules. Upregulating the expression of VpVTC in 'Baihe-35-1' resulted in a gradual increase in the ascorbic acid concentration of leaves inoculated with E. necator. Furthermore, VpVTC was expressed in leaves, stems, inflorescence, tendrils, and fruit at all developmental stages, with the highest level occurring in fruit 35 days after flowering.

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Section: The Roles Of Asa During Pathogenesismentioning

confidence: 87%

Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

Hou

Li²,

Gao³

et al. 2013

Genet. Mol. Res.

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“…AA level was measured by high performance liquid chromatography (HPLC) as described in detail previously [38].…”

Section: Methodsmentioning

confidence: 99%

MiR393 Regulation of Auxin Signaling and Redox-Related Components during Acclimation to Salinity in Arabidopsis

et al. 2014

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One of the most striking aspects of plant plasticity is the modulation of development in response to environmental changes. Plant growth and development largely depend on the phytohormone auxin that exerts its function through a partially redundant family of F-box receptors, the TIR1-AFBs. We have previously reported that the Arabidopsis double mutant tir1 afb2 is more tolerant to salt stress than wild-type plants and we hypothesized that down-regulation of auxin signaling might be part of Arabidopsis acclimation to salinity. In this work, we show that NaCl-mediated salt stress induces miR393 expression by enhancing the transcription of AtMIR393A and leads to a concomitant reduction in the levels of the TIR1 and AFB2 receptors. Consequently, NaCl triggers stabilization of Aux/IAA repressors leading to down-regulation of auxin signaling. Further, we report that miR393 is likely involved in repression of lateral root (LR) initiation, emergence and elongation during salinity, since the mir393ab mutant shows reduced inhibition of emergent and mature LR number and length upon NaCl-treatment. Additionally, mir393ab mutant plants have increased levels of reactive oxygen species (ROS) in LRs, and reduced ascorbate peroxidase (APX) enzymatic activity compared with wild-type plants during salinity. Thus, miR393 regulation of the TIR1 and AFB2 receptors could be a critical checkpoint between auxin signaling and specfic redox-associated components in order to coordinate tissue and time-specific growth responses and tolerance during acclimation to salinity in Arabidopsis.

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“…In our case, the application of ETH may stimulate the catabolism of ascorbic acid (AA) thus leading to an increase of tartaric acid biosynthesis, since ethylene stimulates plant senescence and consequently reduction of AA in leaves (Bartoli, Simontacchi, Montaldi, & Puntarulo, 1996) and probably in other plant organs. One of the most important changes observed during plant senescence is the decline in antioxidant contents and the increase in the steady state of reactive oxygen species and a reduction of AA is considered very important for the plant antioxidant defence (Gergoff, Chaves, & Bartoli, 2010). The decreases of AA may be the consequence of ETH effect on either reducing the biosynthesis or on increasing the degradation through reactions possibly leading to a slight increase of tartaric acid in the berry.…”

Section: Hrms-nmr Covariance Analysesmentioning

confidence: 99%

“…In this regard, it must be considered that ripening of the grapes is characterized by an increase of sugar amounts and a concomitant decrease of organic acids concentrations, especially tartaric acid. However, a recent work on Arabidopsis and spinach leaves showed that leaf AA biosynthesis and content is down-regulated by ethylene application (Gergoff et al, 2010) and AA is the key component for tartaric acid biosynthesis (DeBolt et al, 2006). Since NMR signal intensities of sugars were unaltered by ETH treatments and since tartaric acid was correlated to metabolites characterizing ETH treated samples, it can be concluded that ETH application at late stages does not affect ripening level of the grapes and tartaric acid is somehow involved in secondary metabolic pathways (as in leaves) which deserve deeper investigations.…”

Section: Hrms-nmr Covariance Analysesmentioning

confidence: 99%

On the use of Ethephon as abscising agent in cv. Crimson Seedless table grape production: Combination of Fruit Detachment Force, Fruit Drop and metabolomics

et al. 2015

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Ethylene regulates ascorbic acid content during dark-induced leaf senescence

Cited by 43 publications

References 27 publications

Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules

MiR393 Regulation of Auxin Signaling and Redox-Related Components during Acclimation to Salinity in Arabidopsis

On the use of Ethephon as abscising agent in cv. Crimson Seedless table grape production: Combination of Fruit Detachment Force, Fruit Drop and metabolomics

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