Ethanol toxicity in primary cultures of rat myocardial cells

Chronic Ethanol Exposure Induces Early Epithelial-to-Mesenchymal Transition (EMT) and Premalignant Changes in Gingival Keratinocytes: An In Vitro Model of Very Early Oral Carcinogenesis

“…Morphologically, they exhibit polygonal or rounded cell shapes with an elevated nuclear–cytoplasmic ratio and prominent nucleoli—classical indicators of malignant progression, which are also detected in diverse human OSCC cell lines [ 42 , 118 ]. Cytoplasmic granules may represent autophagic vacuoles, consistent with EtOH-induced autophagy in other tissues [ 119 , 120 ]. Chronic EtOH exposure produced the distinct EPI EtOH phenotype with reduced cell–cell adhesion and the loss of cobblestone morphology, consistent with literature on EtOH-induced membrane alterations [ 121 , 122 , 123 ].…”

Chronic Ethanol Exposure Induces Early Epithelial-to-Mesenchymal Transition (EMT) and Premalignant Changes in Gingival Keratinocytes: An In Vitro Model of Very Early Oral Carcinogenesis

“…Morphologically, they exhibit polygonal or rounded cell shapes with an elevated nuclear–cytoplasmic ratio and prominent nucleoli—classical indicators of malignant progression, which are also detected in diverse human OSCC cell lines [ 42 , 118 ]. Cytoplasmic granules may represent autophagic vacuoles, consistent with EtOH-induced autophagy in other tissues [ 119 , 120 ]. Chronic EtOH exposure produced the distinct EPI EtOH phenotype with reduced cell–cell adhesion and the loss of cobblestone morphology, consistent with literature on EtOH-induced membrane alterations [ 121 , 122 , 123 ].…”

Closed Chamber System for Delivery of Ethanol to Cell Cultures

“…Numerous culture systems have been employed to study the effects of ethanol on different cell types for cytotoxicity (Walker et al, 1974;Seil et al, 1977;Morland and Bessensen, 1977;Scott et al, 1986;Acosta et al, 1986), functional abnormalities (Okarama et al, 1972;Martinez et al, 1977;McCall and Ryan, 1987) and growth mechanisms (Syapin et al, 1976;Mashimo, 1985;Farley et al, 1985;Hassler and Moran, 1986). The methods of delivery of ethanol to the systems are variable and dose concentrations critical for interpreta-tion of results (Syapin and Noble, 1979;Butler et al, 1985;Acosta et al, 1986;Hassler and Moran, 1986;Hurley et al, 1987;McCall and Ryan, 1987). Some report ethanol-induced alterations, but some do not clearly address the methods to assure accuracy and constancy of delivery (Farley et al, 1985).…”

Chronic Ethanol Exposure Induces Early Epithelial-to-Mesenchymal Transition (EMT) and Premalignant Changes in Gingival Keratinocytes: An In Vitro Model of Very Early Oral Carcinogenesis

“…Morphologically, they exhibit polygonal or rounded cell shapes with an elevated nuclear–cytoplasmic ratio and prominent nucleoli—classical indicators of malignant progression, which are also detected in diverse human OSCC cell lines [ 42 , 118 ]. Cytoplasmic granules may represent autophagic vacuoles, consistent with EtOH-induced autophagy in other tissues [ 119 , 120 ]. Chronic EtOH exposure produced the distinct EPI EtOH phenotype with reduced cell–cell adhesion and the loss of cobblestone morphology, consistent with literature on EtOH-induced membrane alterations [ 121 , 122 , 123 ].…”

Closed Chamber System for Delivery of Ethanol to Cell Cultures

“…Numerous culture systems have been employed to study the effects of ethanol on different cell types for cytotoxicity (Walker et al, 1974;Seil et al, 1977;Morland and Bessensen, 1977;Scott et al, 1986;Acosta et al, 1986), functional abnormalities (Okarama et al, 1972;Martinez et al, 1977;McCall and Ryan, 1987) and growth mechanisms (Syapin et al, 1976;Mashimo, 1985;Farley et al, 1985;Hassler and Moran, 1986). The methods of delivery of ethanol to the systems are variable and dose concentrations critical for interpreta-tion of results (Syapin and Noble, 1979;Butler et al, 1985;Acosta et al, 1986;Hassler and Moran, 1986;Hurley et al, 1987;McCall and Ryan, 1987). Some report ethanol-induced alterations, but some do not clearly address the methods to assure accuracy and constancy of delivery (Farley et al, 1985).…”

Chronic Ethanol Exposure Induces Early Epithelial-to-Mesenchymal Transition (EMT) and Premalignant Changes in Gingival Keratinocytes: An In Vitro Model of Very Early Oral Carcinogenesis

“…Morphologically, they exhibit polygonal or rounded cell shapes with an elevated nuclear–cytoplasmic ratio and prominent nucleoli—classical indicators of malignant progression, which are also detected in diverse human OSCC cell lines [ 42 , 118 ]. Cytoplasmic granules may represent autophagic vacuoles, consistent with EtOH-induced autophagy in other tissues [ 119 , 120 ]. Chronic EtOH exposure produced the distinct EPI EtOH phenotype with reduced cell–cell adhesion and the loss of cobblestone morphology, consistent with literature on EtOH-induced membrane alterations [ 121 , 122 , 123 ].…”

Closed Chamber System for Delivery of Ethanol to Cell Cultures

“…Numerous culture systems have been employed to study the effects of ethanol on different cell types for cytotoxicity (Walker et al, 1974;Seil et al, 1977;Morland and Bessensen, 1977;Scott et al, 1986;Acosta et al, 1986), functional abnormalities (Okarama et al, 1972;Martinez et al, 1977;McCall and Ryan, 1987) and growth mechanisms (Syapin et al, 1976;Mashimo, 1985;Farley et al, 1985;Hassler and Moran, 1986). The methods of delivery of ethanol to the systems are variable and dose concentrations critical for interpreta-tion of results (Syapin and Noble, 1979;Butler et al, 1985;Acosta et al, 1986;Hassler and Moran, 1986;Hurley et al, 1987;McCall and Ryan, 1987). Some report ethanol-induced alterations, but some do not clearly address the methods to assure accuracy and constancy of delivery (Farley et al, 1985).…”