Ethanol extracts of Centella Asiatica leaf improves memory performance in rats after chronic stress via reducing nitric oxide and increasing brain-derived neurotrophic factor (BDNF) concentration

Sari, Dwi Cahyani Ratna; Aswin, Soedjono; Susilowati, Rina; Ar-Rochmah, Mawaddah; Prakosa, Djoko; Romi, Muhammad Mansyur; Tranggono, Untung; Arfian, Nur

doi:10.1037/e645302013-003

Cited by 3 publications

(6 citation statements)

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“…in rats following chronic stress affects the concentration of serum and hippocampal brain-derived neurotrophic factor (BDNF), which is an endogenous neuroprotective agent in the brain. [4][5][6] The brain is the central organ for stress adaptation and is also a target of stress. 6 Chronic stress may result in abnormal changes in brain plasticity, including dendritic retraction, neuronal toxicity and suppression of neurogenesis, as well as axospinous synaptic plasticity.…”

Section: Abstrakmentioning

confidence: 99%

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

Sari¹,

Juananda²,

Ar-Rochmah³

et al. 2018

JmedScie

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Chronic stress produces glucocorticoid-induced neurotoxicity that may lead to alterations of the brain-derived neurotrophic factor (BDNF) concentration in the brain. Cerebellum is known to be severely affected by glucocorticoids-associated oxidative damage. Centella asiatica (L.) Urb. may protect neurons from oxidative damage. This study aimed to investigate the effect of ethanolic extract of C. asiatica (L.) Urb. leaf on the rat cerebellar BDNF levels following stress. Twenty young-adult male Sprague Dawley rats were randomly assigned into four experimental groups. The stress control group received aquadest, and the other groups were treated with different doses of the C. asiatica (L.) Urb. extract i.e 150 (CeA150), 300 (CeA300) and 600 (CeA600) mg/kg body weight/ day orally, respectively and followed by chronic footshock stress for 28 days. Upon completion of the experimental period, all animals were sacrificed and the cerebellar was isolated. The BDNF levels from the cerebellar tissue lysate was measured using ELISA. The mean BDNF levels of the cerebellar tissue in the stress control, CeA150, CeA300 and CeA600 groups were 1217.10±301.40; 771.46±241.45; 757.05±268.29; and 627.00±246.02 pg/mL, respectively. Post-hoc analysis showed a significant difference between the control and treatment groups (p< 0.05). In conclusion, the ethanolic extracts of the C. asiatica (L.) Urb. leaf decrease the cerebellar BDNF levels in rats after chronic stress.

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Section: Abstrakmentioning

confidence: 99%

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

Sari¹,

Juananda²,

Ar-Rochmah³

et al. 2018

JmedScie

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“…CA extract has been widely studied in the form of ethanolic (Sari et al, 2014;Sari and Rochmah, 2015;Binti Mohd Yusuf Yeo et al, 2018;Suri et al, 2018;Wong et al, 2019;Wong et al, 2020), methanolic (Veerendra Kumar and Gupta, 2003;Arora et al, 2018) and aqueous (Mitha et al, 2016;Gray et al, 2018c;Chintapanti et al, 2018) extract as well as leaf juice (Rao et al, 2007;Thirawarapan et al, 2019). Of these different preparations of CA, it was found that the ethanolic extract retained the highest amount of the triterpenes asiatic acid and asiaticoside compared to other solvents (Puttarak and Panichayupakaranant, 2013;Gajbhiye et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Neuroactive Effects of Centella asiatica (L.) Urb. : Cognition Cognitive-enhancing effects of CA extract have been described in numerous studies, in both normal animals and models of aging and neurodegenerative disease (Doknark et al, 2014;Sari et al, 2014;Sirichoat et al, 2015;Yolanda et al, 2015;Wong et al, 2019;Sbrini et al, 2020). In early studies, CA extract was found to improve memory and ameliorate biochemical and mitochondrial dysfunction in a mouse model of aging (Kumar et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Mitoprotective Effects of Centella asiatica (L.) Urb.: Anti-Inflammatory and Neuroprotective Opportunities in Neurodegenerative Disease

2021

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Natural products remain a crucial source of drug discovery for accessible and affordable solutions for healthy aging. Centella asiatica (L.) Urb. (CA) is an important medicinal plant with a wide range of ethnomedicinal uses. Past in vivo and in vitro studies have shown that the plant extract and its key components, such as asiatic acid, asiaticoside, madecassic acid and madecassoside, exhibit a range of anti-inflammatory, neuroprotective, and cognitive benefits mechanistically linked to mitoprotective and antioxidant properties of the plant. Mitochondrial dysfunction and oxidative stress are key drivers of aging and neurodegenerative disease, including Alzheimer’s disease and Parkinson’s disease. Here we appraise the growing body of evidence that the mitoprotective and antioxidative effects of CA may potentially be harnessed for the treatment of brain aging and neurodegenerative disease.

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“…Centella asiatica (CeA) is an herbaceous plant that is found in tropical climate countries. It has been widely used as a traditional herbal medicine due to its anti-inflammatory, antioxidant, and neuroprotective effects [ 31 – 34 ]. The main components of CeA are triterpenoids that consist of asiatic acid, madecassic acid, asiatocoside, and madecassicoside.…”

Section: Introductionmentioning

confidence: 99%

“…The main components of CeA are triterpenoids that consist of asiatic acid, madecassic acid, asiatocoside, and madecassicoside. Madecassic acid-contained CeA demonstrated antidiabetic effects through diminished ROS, increased catalase and glutathione, and reduced inflammatory processes [ 34 , 35 ]. In addition, madecassic acid has an important role as the activator of peroxisome proliferator-activated receptor γ (PPAR γ ) through binding in the 5′ upstream element of peroxisome proliferator response element (PPRE).…”

Section: Introductionmentioning

confidence: 99%

Ethanolic Extract of Centella asiatica Treatment in the Early Stage of Hyperglycemia Condition Inhibits Glomerular Injury and Vascular Remodeling in Diabetic Rat Model

Setyaningsih

Arfian

Fitriawan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Diabetes mellitus (DM) is marked by oxidative stress, inflammation, and vascular dysfunction that caused diabetic nephropathy that resulted in end-stage renal disease (ESRD). Vascular dysfunction is characterized by an imbalance in vasoconstrictor and vasodilator agents which underlies the mechanism of vascular injury in DM. Additionally, diminished podocytes correlate with the severity of kidney injury. Podocyturia often precedes proteinuria in several kidney diseases, including diabetic kidney disease. Centella asiatica (CeA) is known as an anti-inflammatory and antioxidant and has neuroprotective effects. This research aimed to investigate the potential effect of CeA to inhibit glomerular injury and vascular remodeling in DM. Methods. The DM rat model was induced through intraperitoneal injection of streptozotocin 60 mg/kg body weight (BW), and then rats were divided into 1-month DM (DM1, n = 5), 2-month DM (DM2, n = 5), early DM concurrent with CeA treatment for 2 months (DMC2, n = 5), and 1-month DM treated with CeA for 1-month (DM1C1, n = 5). The CeA (400 mg/kg BW) was given daily via oral gavage. The control group (Control, n = 5) was maintained for 2 months. Finally, rats were euthanized and kidneys were harvested to assess vascular remodeling using Sirius Red staining and the mRNA expression of superoxide dismutase, podocytes marker, ACE2, eNOS, and ppET-1 using RT-PCR. Results. The DM groups demonstrated significant elevation of glucose level, glomerulosclerosis, and proteinuria. A significant reduction of SOD1 and SOD3 promotes the downregulation of nephrin and upregulation of TRPC6 mRNA expressions in rat glomerular kidney. Besides, this condition enhanced ppET-1 and inhibited eNOS and ACE2 mRNA expressions that lead to the development of vascular remodeling marked by an increase of wall thickness, and lumen wall area ratio (LWAR). Treatment of CeA, especially the DMC2 group, attenuated glomerular injury and showed the reversal of induced conditions. Conclusions. Centella asiatica treatment at the early stage of diabetes mellitus ameliorates glomerulosclerosis and vascular injury via increasing antioxidant enzymes.

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Ethanol extracts of Centella Asiatica leaf improves memory performance in rats after chronic stress via reducing nitric oxide and increasing brain-derived neurotrophic factor (BDNF) concentration

Cited by 3 publications

References 0 publications

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress

Mitoprotective Effects of Centella asiatica (L.) Urb.: Anti-Inflammatory and Neuroprotective Opportunities in Neurodegenerative Disease

Ethanolic Extract of Centella asiatica Treatment in the Early Stage of Hyperglycemia Condition Inhibits Glomerular Injury and Vascular Remodeling in Diabetic Rat Model

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