Estrogenic Joint Effect of BPA and DES on MCF-7 Cells

Abstract

Abstract.Nowadays environmental health problems caused by Endocrine disrupting chemicals (EDCs) really attracted much concern. Bisphenol A (BPA) and diethylstilbestrol (DES), synthetic estrogens that have similar structure, were proved to be related to various type of cancers, including breast cancer. In this paper, cell viability assay was used as evaluation basis to examine the estrogenic effect and joint effect of BPA, DES and 17β-estradiol (E2). The result showed that the proliferation of MCF-7 cells was promoted by environmental relevant doses of BPA and DES. And the more intense effect would be realized when combining BPA with DES. In addition, BPA or DES could strengthen the estrogenic effect of E2. However, for ternary mixture of E2, BPA and DES, all the experimental groups performed an antagonism.