Estrogenic activities of compound GL-1, isolated from Ganoderma lucidum

Abstract

In this study, the estrogenic effect of GL-1, a component of the Ganoderma lucidum, was studied, and the possible mechanism was discussed preliminarily. The binding ability of GL-1 to estrogen receptor was calculated by computer-aided simulation. The effects of GL-1 on the proliferation of estrogen sensitive estrogen receptor (ER) (+) MCF-7 cells and estrogen insensitive ER (-) MDA-MB-231 cells were detected by MTT method. The effects of GL-1 on the proliferation of estrogen-induced MCF-7 cells, and the effects of the estrogen receptor inhibitor ICI182780 on the proliferation of GL-1-induced MCF-7 cells were detected by MTT assay. The expression of ERα and ERβ monoclonal antibody were detected by Western blot. The results showed that GL-1 has a good binding ability to estrogen receptor β, and has estrogen-like effect, which might be related to secretion of estrogen and expression of ERβ by binding to ERs.

Anti-SARS-CoV-2 potential of Cissampelos pareira L. identified by connectivity map-based analysis and in vitro studies

“…It has been shown that estrogen can regulate the expression of ACE-2 receptors [ 5 ]. Although a few known herbal medicines such as extracts of Ganoderma lucidum , Perilla frutescens , and Mentha haplocalyx have been shown to be effective against SARS-CoV-2 infection [ 33 ], only Ganoderma lucidum has shown effect on estrogen modulation [ 34 ]. Since Cipa appears to have ESR1 modulatory effects [ 10 ], and has been shown to have antiviral potential [ 8 ], we hypothesized it may have inhibitory effect on the novel coronavirus.…”