Estrogen and progesterone regulate expression of the endothelins in the rhesus macaque endometrium

Keator, Christopher S.; Mah, Kunie; Ohm, Lindsay; Slayden, Ov D.

doi:10.1093/humrep/der115

Cited by 13 publications

(14 citation statements)

References 53 publications

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“…There is increasing evidence that NF-κB signaling is an important player in cancer development and progression 23 . NF-κB signaling in cancer cells can be activated by factors such as IL-1β, TNFα and IL-8, IL-6 and MCP-1 secreted by cells in the microenvironment of the tumor, e.g.…”

Section: Discussionmentioning

confidence: 99%

Adipocytes induce distinct gene expression profiles in mammary tumor cells and enhance inflammatory signaling in invasive breast cancer cells

Nickel

Blücher

Kadri

et al. 2018

Sci Rep

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Obesity is a known risk factor for breast cancer. Since obesity rates are constantly rising worldwide, understanding the molecular details of the interaction between adipose tissue and breast tumors becomes an urgent task. To investigate potential molecular changes in breast cancer cells induced by co-existing adipocytes, we used a co-culture system of different breast cancer cell lines (MCF-7 and T47D: ER+/PR+/HER2− and MDA-MB-231: ER−/PR−/HER2−) and murine 3T3-L1 adipocytes. Here, we report that co-culture with adipocytes revealed distinct changes in global gene expression pattern in the different breast cancer cell lines. Our microarray data revealed that in both ER+ cell lines, top upregulated genes showed significant enrichment for hormone receptor target genes. In triple-negative MDA-MB-231 cells, co-culture with adipocytes led to the induction of pro-inflammatory genes, mainly involving genes of the Nf-κB signaling pathway. Moreover, co-cultured MDA-MB-231 cells showed increased secretion of the pro-inflammatory interleukins IL-6 and IL-8. Using a specific NF-κB inhibitor, these effects were significantly decreased. Finally, migratory capacities were significantly increased in triple-negative breast cancer cells upon co-culture with adipocytes, indicating an enhanced aggressive cell phenotype. Together, our studies illustrate that factors secreted by adipocytes have a significant impact on the molecular biology of breast cancer cells.

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Section: Discussionmentioning

confidence: 99%

Adipocytes induce distinct gene expression profiles in mammary tumor cells and enhance inflammatory signaling in invasive breast cancer cells

Nickel

Blücher

Kadri

et al. 2018

Sci Rep

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“…ATP6V0A4 mediates invasion of breast cancer cell lines in the transwell Matrigel assay (Hinton et al, 2009). EDN3 has been suggested as a tumor suppressor gene in the human mammary gland (Wiesmann et al, 2009) and its expression was shown to be regulated by estrogen and progesterone in the rhesus macaque (Keator et al, 2011). LTF (Lactotransferrin) is an estrogen-inducible gene (Das et al, 1998; Ghosh et al, 1999; Moggs et al, 2004; Stuckey et al, 2006); its protein product exists in high concentration in human milk (Hakansson et al, 1995).…”

Section: Resultsmentioning

confidence: 99%

Sexual dimorphism in gene expression and regulatory networks across human tissues

Chen

Lopes‐Ramos

Kuijjer

et al. 2016

Preprint

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SummarySexual dimorphism manifests in many diseases and may drive sex-specific therapeutic responses. To understand the molecular basis of sexual dimorphism, we conducted a comprehensive assessment of gene expression and regulatory network modeling in 31 tissues using 8716 human transcriptomes from GTEx. We observed sexually dimorphic patterns of gene expression involving as many as 60% of autosomal genes, depending on the tissue. Interestingly, sex hormone receptors do not exhibit sexually dimorphic expression in most tissues; however, differential network targeting by hormone receptors and other transcription factors (TFs) captures their downstream sexually dimorphic gene expression. Furthermore, differential network wiring was found extensively in several tissues, particularly in brain, in which not all regions exhibit strong differential expression. This systems-based analysis provides a new perspective on the drivers of sexual dimorphism, one in which a repertoire of TFs plays important roles in sex-specific rewiring of gene regulatory networks.HighlightsSexual dimorphism manifests in both gene expression and gene regulatory networksSubstantial sexual dimorphism in regulatory networks was found in several tissuesMany differentially regulated genes are not differentially expressedSex hormone receptors do not exhibit sexually dimorphic expression in most tissues

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“…Total RNA (1 mg) was reverse transcribed into cDNA, in the presence of random hexamer primers (Promega Corporation, Madison, WI, USA) using an Omniscript RT kit (Qiagen). Real-time PCRs were on a 7500 Fast Real-time PCR System (ABI; Applied Biosystems, Carlsbad, CA, USA) as described previously (Keator et al, 2011). Real-time PCRs were performed in duplicate on 2 ml of diluted (1:20) cDNA and compared against a five-point standard curve of pooled endometrial cDNA.…”

Section: Real-time Pcrmentioning

confidence: 99%

“…Real-time PCRs were performed in duplicate on 2 ml of diluted (1:20) cDNA and compared against a five-point standard curve of pooled endometrial cDNA. Each reaction contained 250-nM TaqManw probe specific for rhesus macaque MMP-26 (FAM-labeled) and probe (VIC-labeled) specific for the ribosomal protein gene RPL32 or mitochondrial ribosomal protein S10, described previously (Keator et al, 2011). The reactions contained 900-nM MMP-26 or 300-nM housekeeping gene sense and antisense primers and 5 ml of 2× Fast Universal PCR Master Mix (ABI).…”

Section: Real-time Pcrmentioning

confidence: 99%

“…Representative slides were also incubated with sense probe as another negative control. The slides were treated with RNase A at 378C for 30 min to inactivate non-hybridized probe and then subjected to high stringency rinses in a descending series of SSC as recently described (Keator et al, 2011). Sections were dehydrated in an ascending series of alcohol dilutions, vacuum dried, coated with NTB autoradiographic emulsion (Eastman Kodak, Rochester, NY, USA), stored at 48C for 9 -10 days, developed in aqueous D-19 (Eastman Kodak), lightly counterstained with hematoxylin, dehydrated in an ascending series of alcohol dilutions, cleared with xylene and cover slipped with Permount (Fisher Scientific).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

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Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque

Almeida-Francia

Keator²,

Mah

et al. 2012

Human Reproduction

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Endometrial MMP-26 expression is dependent on the presence of progesterone in the early secretory phase and then gradually becomes refractory to progesterone stimulation in the late secretory phase. In the macaque, MMP-26 is a marker of the pre-decidual, secretory endometrium. During the second half of the late secretory phase, and during decidualization, MMP-26 loses its response to progesterone concurrent with the loss of epithelial PGR. The decline in MMP-26 levels between the mid- and late secretory phases may play a role in the receptive window for embryo implantation.

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Estrogen and progesterone regulate expression of the endothelins in the rhesus macaque endometrium

Abstract: Our results indicate that estrogen and progesterone regulate the EDN family during the menstrual cycle. The changes in the EDN paracrine system during the mid-secretory phase may indicate a role for EDN during embryo implantation.

Cited by 13 publications

References 53 publications

Adipocytes induce distinct gene expression profiles in mammary tumor cells and enhance inflammatory signaling in invasive breast cancer cells

Adipocytes induce distinct gene expression profiles in mammary tumor cells and enhance inflammatory signaling in invasive breast cancer cells

Sexual dimorphism in gene expression and regulatory networks across human tissues

Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque

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