Estrogen and/or Progesterone Effects on HepG2 Human Cell Lines; Oxidant or Antioxidant?

Zal, Fatemeh; Miladpour, Behnoosh; Taheri, Reihane; Heidari, Iman; Mostafavi‐Pour, Zohreh

doi:10.18869/nrip.jamsat.1.1.35

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…The HEPES-supplemented Krebs–Henseleit buffer (pH 7.4, KHB) was freshly prepared using HEPES (5.95 g/L), d -glucose (2.0 g/L), magnesium sulfate (0.141 g/L), potassium dihydrogenphosphate (0.16 g/L), potassium chloride (0.35 g/L), sodium chloride (6.9 g/L), calcium chloride dihydrate (0.373 g/L), and sodium bicarbonate (2.1 g/L), followed by adjustment to pH 7.4 with NaOH. The utilized substances within this study were neither found (see Supplementary Materials, Figure S14 ) nor reported [ 31 , 32 , 33 , 34 , 35 ] to be cytotoxic within the utilized concentration range and incubation duration.…”

Section: Methodscontrasting

confidence: 60%

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Investigation of Radiotracer Metabolic Stability In Vitro with CYP-Overexpressing Hepatoma Cell Lines

Lemm

Köhler

Wodtke

et al. 2022

Cells

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The characterization of novel radiotracers toward their metabolic stability is an essential part of their development. While in vitro methods such as liver microsome assays or ex vivo blood or tissue samples provide information on overall stability, little or no information is obtained on cytochrome P450 (CYP) enzyme and isoform-specific contribution to the metabolic fate of individual radiotracers. Herein, we investigated recently established CYP-overexpressing hepatoblastoma cell lines (HepG2) for their suitability to study the metabolic stability of radiotracers in general and to gain insight into CYP isoform specificity. Wildtype HepG2 and CYP1A2-, CYP2C19-, and CYP3A4-overexpressing HepG2 cells were incubated with radiotracers, and metabolic turnover was analyzed. The optimized protocol, covering cell seeding in 96-well plates and analysis of supernatant by radio thin-layer-chromatography for higher throughput, was transferred to the evaluation of three 18F-labeled celecoxib-derived cyclooxygenase-2 inhibitors (coxibs). These investigations revealed time-dependent degradation of the intact radiotracers, as well as CYP isoform- and substrate-specific differences in their metabolic profiles. HepG2 CYP2C19 proved to be the cell line showing the highest metabolic turnover for each radiotracer studied here. Comparison with human and murine liver microsome assays showed good agreement with the human metabolite profile obtained by the HepG2 cell lines. Therefore, CYP-overexpressing HepG2 cells provide a good complement for assessing the metabolic stability of radiotracers and allow the analysis of the CYP isoform-specific contribution to the overall radiotracer metabolism.

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Section: Methodscontrasting

confidence: 60%

“…Cell growth and vitality of CYP-overexpressing cell lines. References mentioned in SM: [ 34 ] as in reference list below.…”

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confidence: 99%

Investigation of Radiotracer Metabolic Stability In Vitro with CYP-Overexpressing Hepatoma Cell Lines

Lemm

Köhler

Wodtke

et al. 2022

Cells

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“…The enzymatic antioxidants including SOD, GSH-Px, and CAT act as the first defense against formation of free radicals by catalyzing their conversion to less reactive or inert species [ 34 , 35 ]. In this study, the changes in the antioxidant enzymatic activities (SOD, GSH-Px) were consistent with those reported by Radin et al, further indicating that the dairy goats in their late pregnancy and early lactation may have experienced some degree of lipid peroxidation [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Oxidative status in dairy goats: periparturient variation and changes in subclinical hyperketonemia and hypocalcemia

et al. 2021

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Background A better comprehension of the redox status during the periparturient period may facilitate the development of management and nutritional solutions to prevent subclinical hyperketonemia (SCHK) and subclinical hypocalcemia (SCHC) in dairy goats. We aimed to evaluate the variation in the redox status of dairy goats with SCHK and SCHC during their periparturient periods. Guanzhong dairy goats (n = 30) were assigned to SCHK (n = 10), SCHC (n = 10), and healthy (HEAL, n = 10) groups based on their blood β-hydroxybutyrate (BHBA) and calcium (Ca) concentrations. Blood were withdrawn from goats every week from 3 weeks before the expected parturition date to 3 weeks post-kidding. On the same day, the body condition scores (BCS) were evaluated, and the milk yield was recorded for each goat. The metabolic profile parameters and the indicators of oxidative status were determined by using the standard biochemical techniques. Results In comparison with the HEAL goats, SCHK and SCHC goats presented with a more dramatic decline of BCS post-kidding and a significant decrease in the milk yield at 2- and 3-weeks postpartum, ignoring the obvious increase at 1-week postpartum. The levels of non-esterified fatty acids (NEFA) peaked at parturition, exhibiting significantly higher levels from 1-week prepartum to the parturition day in the SCHK and SCHC groups. The malondialdehyde (MDA) concentration was increased in the SCHK goats from 1-week antepartum until 3-weeks postpartum, with its concentration being significantly higher in the SCHC goats at parturition. The hydrogen peroxide (H2O2) concentration was significantly lower in the SCHK and SCHC goats from 2-weeks antepartum to 1-week post-kidding. The total antioxidant capacity (T-AOC) and the superoxide dismutase (SOD) level were decreased at 1-week antepartum in the SCHK and SCHC goats, respectively. The glutathione peroxidase (GSH-Px) level was increased in the SCHK and SCHC goats during the early lactation period. Conclusions The SCHK and SCHC goats exerted more efforts to maintain their redox homeostasis and to ensure the production performance than the HEAL goats during their periparturient period, probably owing to more intense fat mobilization and lipid peroxidation in the former.

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Estrogen and/or Progesterone Effects on HepG2 Human Cell Lines; Oxidant or Antioxidant?

Cited by 2 publications

References 21 publications

Investigation of Radiotracer Metabolic Stability In Vitro with CYP-Overexpressing Hepatoma Cell Lines

Investigation of Radiotracer Metabolic Stability In Vitro with CYP-Overexpressing Hepatoma Cell Lines

Oxidative status in dairy goats: periparturient variation and changes in subclinical hyperketonemia and hypocalcemia

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