Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Kaczyński, Piotr; Baryla, Monika; Goryszewska, Ewelina; Wacławik, Agnieszka

doi:10.3390/ijms22073655

Cited by 5 publications

(4 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings are also consistent with a report indicating that the expression of DNMTs in the human endometrium decreased during the secretory phase and was reduced by E 2 and P4 in endometrial stromal cells [ 42 ]. Similarly, our recent findings indicate that E 2 controlled DNMT expression in the porcine corpus luteum in vivo and changes in their abundance were significant during pregnancy [ 31 ]. Interestingly, recent studies have indicated that E 2 can affect the expression of DNMTs via a complex mechanism involving a number of co-regulators [ 43 ].…”

Section: Discussionmentioning

confidence: 71%

See 1 more Smart Citation

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Kaczyński

Weijden

Goryszewska-Szczurek

et al. 2022

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

During early pregnancy, porcine conceptuses (the embryos with associated membranes) secrete estradiol-17β (E2)—their major signal for maternal recognition of pregnancy—and prostaglandin E2 (PGE2). Both hormones induce prominent changes of the endometrial transcriptome in vivo. Studies on endometrial pathologies have shown that E2 affects gene expression by epigenetic mechanisms related to DNA methylation. Herein, we determined the effects of E2 and PGE2 alone, and a combined E2 + PGE2 treatment administered into the uterine lumen in vivo on the expression and activity of DNA-methyltransferases (DNMT) and on CpG methylation patterns of selected genes in porcine endometrium. To compare the effect of treatment with the physiological effect of pregnancy, endometria from day 12 pregnant/cyclic gilts were included. Both E2 and PGE2 significantly reduced the expression of DNMTs. Likewise, the expressions of DNMT1 and DNMT3A were decreased on day 12 of pregnancy compared to the estrous cycle. DNMT activity increased in endometrial samples following E2 treatment and in gilts on day 12 of pregnancy. Treatment with E2 alone and/or simultaneously with PGE2 altered endometrial DNA methylation of CpG sites of ADAMTS20, ADH1C, BGN, PSAT1 and WNT5A. Different CpG methylation patterns of ADAMTS20, BGN, DMBT1, RASSF1 and WNT5A were found in the endometrium on day 12 of pregnancy compared to day 12 of the estrous cycle. Significant correlations were detected between CpG methylation and gene expression for ADAMTS20, ADH1C, BGN, DMBT1, PSAT1 and WNT5A. Our results indicate that CpG methylation induced by embryonic signals may contribute to regulating endometrial gene expression during pregnancy establishment.

show abstract

Section: Discussionmentioning

confidence: 71%

“…The endometrial DNMT1 , DNMT3A , and DNMT3B gene expression were determined using quantitative PCR (qPCR, real-time RT-PCR), as described previously [ 31 ]. Detailed information of qPCR procedure has been provided in Supplementary Information.…”

Section: Methodsmentioning

confidence: 99%

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Kaczyński

Weijden

Goryszewska-Szczurek

et al. 2022

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…The role of E2 in the luteal function in pigs is not clearly understood. Whereas some studies report its beneficial effect on CL function [ 45 , 46 ], others suggest E2 to play a role in CL regression [ 22 , 47 ]. Our results suggest that actions of E2 must be dependent upon the expression of E2-metabolizing enzymes, which differ in abundance during the estrous cycle.…”

Section: Discussionmentioning

confidence: 99%

Changes in Porcine Corpus Luteum Proteome Associated with Development, Maintenance, Regression, and Rescue during Estrous Cycle and Early Pregnancy

Likszo

Skarzynski

Jalali

2021

IJMS

View full text Add to dashboard Cite

Corpus luteum (CL), a transitory gland, undergoes rapid growth in a limited time to produce progesterone (P4) followed by its regression. A complex molecular signaling is involved in controlling luteal P4 production. In the present study, 2D gel electrophoresis-based proteomics and in silico functional analysis were used to identify changes in key proteins and pathways in CL along the different stages of the estrous cycle as its development progresses from early (Day 3) to mid-luteal phase (Day 9), effective functioning (Day 12) followed by regression (Day 15) or, in the case of pregnancy, rescue of function (Day 15). A total of 273 proteins were identified by MALDI-MS/MS analysis that showed significant changes in abundances at different stages of CL development or regression and rescue. Functional annotation of differentially abundant proteins suggested enrichment of several important pathways and functions during CL development and function maintenance including cell survival, endocytosis, oxidative stress response, estradiol metabolism, and angiogenesis. On the other hand, differentially abundant proteins during CL regression were associated with decreased steroid synthesis and metabolism and increased apoptosis, necrosis, and infiltration of immune cells. Establishment of pregnancy rescues CL from regression by maintaining the expression of proteins that support steroidogenesis as pathways such as the super-pathway of cholesterol biosynthesis, RhoA signaling, and functions such as fatty acid metabolism and sterol transport were enriched in CL of pregnancy. In this study, some novel proteins were identified along CL development that advances our understanding of CL survival and steroidogenesis.

show abstract

“…Maternal–fetal exchange of information is reportedly crucial for embryo implantation. During implantation, the trophoblast cells in the blastocyst create tissue and physiological connections with the endometrial epithelium, which stabilize the development of the blastocyst in the uterus [ 7 , 8 ]. The critical phase in pigs is day 12 of gestation (gd12), whereby the embryonic morphology changes dramatically from tubular to filamentous, accompanied by the secretion of large amounts of estrogen and interleukins; estrogen levels peak on gd12 and can be harnessed to validate pregnancy to facilitate embryo implantation [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Ding

Gan

et al. 2022

Animals

View full text Add to dashboard Cite

Extracellular vesicles (EVs) in uterine luminal fluid (ULF) can reportedly affect the proliferation and migration function of porcine trophoblast cells (PTr2 cells) by mediating the maternal–fetal exchange of information. miR-143-3p is considered a crucial miRNA in early pregnancy in mammals; however, little is currently known about how it regulates the function of PTr2 cells. This study aimed to investigate the effects of ssc-miR-143-3p in ULF-EVs on the function of PTr2 cells during porcine embryo implantation. The uptake of ULF-EVs by PTr2 cells was confirmed, which significantly increased the expression of ssc-miR-143-3p. Ssc-miR-143-3p was found to facilitate the proliferation and migration of PTr2 cells in the CCK-8, EdU and wound-closure assays, while the opposite findings were observed after the knockdown of ssc-miR-143-3p. Bioinformatics analysis and the luciferase reporter assay showed that glycerol-3 phosphate dehydrogenase 2 (GDP2) was directly targeted by miR-143-3p. Inhibition of miR-143-3p was validated in mice to inhibit embryo implantation. In summary, ssc-miR-143-3p in ULF-EVs affects the proliferation and migration of PTr2 cells by mediating GPD2, thereby affecting embryo implantation.

show abstract

Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Cited by 5 publications

References 55 publications

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Novel role for conceptus signals in mRNA expression regulation by DNA methylation in porcine endometrium during early pregnancy

Changes in Porcine Corpus Luteum Proteome Associated with Development, Maintenance, Regression, and Rescue during Estrous Cycle and Early Pregnancy

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells

Contact Info

Product

Resources

About