Estimation of SLE Activity Based on the Serum Level of Chosen Cytokines and Superoxide Radical Generation

Waszczykowska, Elżbieta; Robak, Ewa; Woźniacka, Anna; Torzecka, Jolanta Dorota; Sysa-Jędrzejowska, Anna

doi:10.1080/09629359990586

Cited by 33 publications

(23 citation statements)

References 27 publications

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“…Spontaneous non-controlled reactions produce excessive amounts of active oxygen forms and cause defects of protective enzymes, may be one of the pathogenic elements in inXammatory diseases [1,3,4]. The most common reactive oxygen forms in the human organism are superoxide anion radical (O 2 ¡ ), hydroxyl radical (OH ¡ ), hydrogen peroxide (H 2 O 2 ), and nitrogen oxide (NO).…”

Section: Introductionmentioning

confidence: 99%

“…InXammatory responses triggered by local formation and deposition of antigen-antibody immune complexes are responsible for the majority of the clinical manifestations [1].…”

Section: Introductionmentioning

confidence: 99%

“…The most common reactive oxygen forms in the human organism are superoxide anion radical (O 2 ¡ ), hydroxyl radical (OH ¡ ), hydrogen peroxide (H 2 O 2 ), and nitrogen oxide (NO). The mechanism of destruction of cells and tissues caused by superoxide radicals is based on both direct toxic inXuence on the cells and indirect eVect via the production of other biologically active compounds [1,5].…”

Section: Introductionmentioning

confidence: 99%

“…Active oxygen forms produced in the processes of biological oxygenation and reduction have been studied in adult onset SLE [1,2]. Spontaneous non-controlled reactions produce excessive amounts of active oxygen forms and cause defects of protective enzymes, may be one of the pathogenic elements in inXammatory diseases [1,3,4].…”

Section: Introductionmentioning

confidence: 99%

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Superoxide release in juvenile systemic lupus erythematosus

et al. 2011

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The objective of this study was to analyze the un-stimulated and stimulated release of superoxide anion (O(2) (-)) by granulocytes and monocytes in juvenile systemic lupus erythematosus (jSLE). The un-stimulated and phorbol myristate acetate (PMA, 30 nM)-induced O(2) (-)by granulocytes and monocytes were determined in six different times of incubation in patients with 23 jSLE and 28 controls. The analysis compared the jSLE group, which was classified into two subgroups by SLEDAI in one inactive subgroup (score <3) (n = 13 patients) and one active subgroup (score ≥3) (n = 10 patients) to the same control group. At time of blood withdrawal, 13 (56.52%) had inactive and 10 (43.47%) patients had active SLE. jSLE patients' granulocytes and monocytes had always a lower un-stimulated O(2) (-) production when compared to controls. Stimulated granulocytes had an increased O(2) (-) production at baseline followed by a significant lower production at 60 min in jSLE when compared to controls. Stimulated monocytes had a similar O(2) (-) production among patients with jSLE and controls. The results suggest a defect in phagocytic function in jSLE. The significant higher release of O(2) (-) in the assays of the stimulated granulocytes, in the initial instances, the so-called respiratory burst, could be attributed to the inflammatory state of phagocytes.

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Section: Introductionmentioning

confidence: 99%

“…InXammatory responses triggered by local formation and deposition of antigen-antibody immune complexes are responsible for the majority of the clinical manifestations [1].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Superoxide release in juvenile systemic lupus erythematosus

et al. 2011

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“…16 Waszczykowska et al, 17 suggested that intracellular free radicals are capable of inducing cytokine synthesis that participate and modulate infl ammatory responses with the creation of superoxide radicals.…”

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confidence: 99%

Malondialdeído e grupo sulfidrila como biomarcadores do estresse oxidativo em pacientes com lúpus eritematoso sistêmico

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Netto

et al. 2012

Rev. Bras. Reumatol.

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Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus

Morgan¹,

Sturgess²,

Davies³

2005

Arthritis & Rheumatism

119

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Objective. To examine protein oxidation in systemic lupus erythematosus (SLE) and to correlate levels of protein oxidation products with disease activity.Methods. Serum was collected from SLE patients and healthy control subjects. Protein-bound carbonyls and the pro-oxidant enzyme myeloperoxidase (MPO) were quantified by enzyme-linked immunosorbent assay. Protein thiols were quantified using 5,5-dithionitrobenzoic acid. Protein-bound amino acids and methionine, tyrosine, and phenylalanine oxidation products were quantified by acid hydrolysis and highperformance liquid chromatography. Disease activity was assessed by the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Levels of antidouble-stranded DNA (anti-dsDNA) antibodies were measured by radioimmunoassay.Results. Compared with control subjects, SLE patients exhibited elevated levels of protein carbonyls (0.108 ؎ 0.078 versus 0.064 ؎ 0.028 nmoles/mg of protein; P ‫؍‬ 0.046), decreased levels of protein thiols (3.9 ؎ 1.1 versus 4.9 ؎ 0.7 nmoles/mg of protein; P ‫؍‬ 0.003), decreased levels of protein-bound methionine (P ‫؍‬ 0.0007), and increased levels of protein-bound methionine sulfoxide (P ‫؍‬ 0.0043) and 3-nitrotyrosine (P ‫؍‬ 0.0477). SLE patients with high SLEDAI scores or elevated anti-dsDNA antibody levels exhibited increased oxidation compared with patients with low SLEDAI scores or low antibody levels. Serum MPO levels were decreased in SLE patients (P ‫؍‬ 0.03), suggesting that this enzyme is not responsible for the enhanced protein oxidation.Conclusion. We found elevated levels of multiple markers of protein oxidation in sera from SLE patients compared with controls, and these levels correlated with disease activity. The findings suggest that protein oxidation may play a role in the pathogenesis of chronic organ damage in SLE.

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Estimation of SLE Activity Based on the Serum Level of Chosen Cytokines and Superoxide Radical Generation

Cited by 33 publications

References 27 publications

Superoxide release in juvenile systemic lupus erythematosus

Superoxide release in juvenile systemic lupus erythematosus

Malondialdeído e grupo sulfidrila como biomarcadores do estresse oxidativo em pacientes com lúpus eritematoso sistêmico

Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus

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