Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.12.088 View full text
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Kanta Horie, Yoshiaki Sato, Takayuki Kimura, Tatsuji Nakamura, Yasushi Ishihama, Yoshiya Oda, Tohru Ikegami, Nobuo Tanaka

Abstract: An estimation of the performance and optimization of gradient HPLC conditions using various columns for maximum total peak capacity was studied for "one-shot proteomics" which involves one-dimensional gradient HPLC, connected to an electrospray ionization (ESI)-mass spectrometry (MS), using a monolithic silica-C₁₈ capillary column (350 cm long and 100 μm internal diameter) and an over 40 h shallow gradient elution with one injection. Optimization of such special one-dimensional HPLC has been a tedious task if …

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